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Assessment of chelation of cupric ions with flavones by use of hematoxylin method
Tamborová, Barbora ; Karlíčková, Jana (advisor) ; Sklenářová, Hana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical botanic and ecology Candidate: Mgr. Barbora Tamborová Consultant: PharmDr. Jana Karlíčková, Ph.D. Title of Thesis: Assessment of chelation of cupric ions with flavones by use of hematoxylin method Copper is an essential trace element involved in many physiological processes. It is a part of several enzymes. Disruption of copper homeostasis results in pathological changes and diseases, excess of copper is highly toxic for human body. Flavonoids, the ubiquitous plant secondary metabolites, are highly abundant in the human diet. They represent a large group of polyphenolic compounds. They are characterized by a broad spectrum of biological activities. The anti-inflammatory, antiallergic, antimicrobial, hepatoprotective, immunomodulatory, cytotoxic and anticancer effects of flavonoids are well documented. Flavones belong to the group of flavonoids, they have chelating and antioxidative properties. They are known for their anti-inflammatory, antibacterial, antiviral and antiallergic activity. This thesis deals with chelating activity of six selected flavones, substituted on the A ring, namely 5-hydroxyflavone, baicalein, baicalin, chrysin, mosloflavone and negletein. Individual analyses were preformed using...
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Interaction of phenylpropionic acids with copper
Zemanová, Kamila ; Karlíčková, Jana (advisor) ; Matysová, Ludmila (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Kamila Zemanová Supervisor: PharmDr. Jana Karlíčková, Ph.D. Title of Thesis: Interaction of phenylpropionic acids with copper Copper is an essential trace element which is essential for our body. It has a significant effect on the correct functioning of important organs and it plays an important role in the transfer of electrons at the major enzymatic pathways as a prostetic group. On the other hand, excess or deficiency of copper in the human body can cause many diseases. Phenylpropionic acids are group of substances which can have antioxidant, anti-inflammatory and anticancer effects. They are involved in the scavenging of free radicals and reactive oxygen species. In this diploma thesis, I tested copper chelating activity of six phenylpropionic acids at different pHs by spectrofotometric methods using a hematoxylin and a bathocuproinedisulfonic acid disodium salt as an indicator. 3-(3,4-dihydroxyphenyl)propionic acid showed the highest chelating potential but only using a hematoxylin. KEYWORDS: Copper, Phenylpropionic acids, Antioxidants, Chelating activity, Hematoxylin, Bathocuproin
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Interactions of tamarixetin and isorhamnetin with copper
Lomozová, Zuzana ; Karlíčková, Jana (advisor) ; Chocholouš, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany Candidate: Zuzana Lomozová Supervisor: PharmDr. Jana Karlíčková, Ph.D. Title of Thesis: Interactions of tamarixetin and isorhamnetin with copper Keywords: tamarixetin, isorhamnetin, chelation, reduction, copper, bathocuproine, hematoxylin Copper is a biogenic trace element important for proper function of human organism. It is an essential part of several enzymes and is involved in metabolic processes in the body. Excess or lack of serum copper can lead to pathological conditions. Copper chelating agents are used to treat copper toxic effects. Flavonoids are polyphenolic substances belonging to secondary metabolites of various plants. They are part of the human diet and have a positive impact on our health. They exhibit antioxidant and anti-inflammatory effects and are able to chelate transient metals, especially iron and copper. Chelation therapy is currently used in Wilson's disease in which copper is overloaded. In the future, the chelating effects of flavonoids could be used to treat neurodegenerative diseases, cardiovascular diseases or cancer. In this diploma thesis, interactions of two flavonoids (namely tamarixetin and isorhamnetin) with copper ions in different buffers were tested....
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Interference of reducing agents with the hematoxylin assay for determination of cupric chelation
Zsáková, Emily ; Mladěnka, Přemysl (advisor) ; Vorčáková, Katarína (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Emily Zsáková Supervisor: Assoc. Prof. Přemysl Mladěnka, PharmD., Ph.D. Title of diploma thesis: Interference of reducing agents with the hematoxylin assay for determination of cupric chelation Copper is an essential trace element of living organisms. Disorders of its homeostasis affect various pathological conditions. In excessive amounts, it is toxic due to its ability to form free radicals. Vitamin C, trolox (a water soluble form of vitamin E), glutathione and hydroxylamine possess reducing properties that protect our body from oxidative tissue damage. Oxidized glutathione (GSSG) was also tested for comparison. The object of study was to test the chelating ability of these substances. A simple and fast spectrophotometric method was used. The combination of reducing properties of copper and chelating agents could lead to an improvement in chelation therapy either in heavy metal poisoning, treatment of Wilson's disease, or other diseases. We found that all substances were chelators/copper-chelating agents in vitro in a slightly competitive environment, using hematoxylin as an indicator. However, by using the bathocuproine assays to verify chelating properties, none of the substances...
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Comparison of interactions of equol and desmethylangolensin with iron and copper
Němcová, Hana ; Karlíčková, Jana (advisor) ; Matysová, Ludmila (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany Candidate: Hana Němcová Supervisor: PharmDr. Jana Karlíčková, Ph.D. Title of Thesis: Comparison of interactions of equol and desmethylangolensin with iron and copper Iron and copper are essential trace elements, which are important for our body. Both elements have a significant effect on the correct function of organs and make part of many enzymes. They are able to accept or donate electrons - conversion between oxidized (Fe3+, Cu2+) and reduced (Fe2+, Cu+) forms. If these metals are excess in organism, they are accumulated in the cells and mediate the creation of free radicals, that destroy cell structures. This deficiency is treated with chelators, which facilitate the excretion of metals from the body. Isoflavonoids are polyfenolic substances, which can have antioxidant effects and they are involved in the scavening of free radicals. Isoflavonoids can have also a pro-oxidative effect, because they are able to reduce metal ions. In this study were tested interactions (chelation and reduction) between the metabolites of isoflavonoids (equol and desmethylangolensin) and ions of iron and copper. Both metabolites only weakly chelate metal ions, but they significantly reduce cupric ions. KEYWORDS: Iron,...
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Interaction of phenylpropionic acids with copper
Zemanová, Kamila ; Karlíčková, Jana (advisor) ; Matysová, Ludmila (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Kamila Zemanová Supervisor: PharmDr. Jana Karlíčková, Ph.D. Title of Thesis: Interaction of phenylpropionic acids with copper Copper is an essential trace element which is essential for our body. It has a significant effect on the correct functioning of important organs and it plays an important role in the transfer of electrons at the major enzymatic pathways as a prostetic group. On the other hand, excess or deficiency of copper in the human body can cause many diseases. Phenylpropionic acids are group of substances which can have antioxidant, anti-inflammatory and anticancer effects. They are involved in the scavenging of free radicals and reactive oxygen species. In this diploma thesis, I tested copper chelating activity of six phenylpropionic acids at different pHs by spectrofotometric methods using a hematoxylin and a bathocuproinedisulfonic acid disodium salt as an indicator. 3-(3,4-dihydroxyphenyl)propionic acid showed the highest chelating potential but only using a hematoxylin. KEYWORDS: Copper, Phenylpropionic acids, Antioxidants, Chelating activity, Hematoxylin, Bathocuproin
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Interaction of phenylpropionic acids with copper
Zemanová, Kamila ; Karlíčková, Jana (advisor) ; Matysová, Ludmila (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Kamila Zemanová Supervisor: PharmDr. Jana Karlíčková, Ph.D. Title of Thesis: Interaction of phenylpropionic acids with copper Copper is an essential trace element which is essential for our body. It has a significant effect on the correct functioning of important organs and it plays an important role in the transfer of electrons at the major enzymatic pathways as a prostetic group. On the other hand, excess or deficiency of copper in the human body can cause many diseases. Phenylpropionic acids are group of substances which can have antioxidant, anti-inflammatory and anticancer effects. They are involved in the scavenging of free radicals and reactive oxygen species. In this diploma thesis, I tested copper chelating activity of six phenylpropionic acids at different pHs by spectrofotometric methods using a hematoxylin and a bathocuproinedisulfonic acid disodium salt as an indicator. 3-(3,4-dihydroxyphenyl)propionic acid showed the highest chelating potential but only using a hematoxylin. KEYWORDS: Copper, Phenylpropionic acids, Antioxidants, Chelating activity, Hematoxylin, Bathocuproin
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Assessment of chelation of cupric ions with flavones by use of hematoxylin method
Tamborová, Barbora ; Karlíčková, Jana (advisor) ; Sklenářová, Hana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical botanic and ecology Candidate: Mgr. Barbora Tamborová Consultant: PharmDr. Jana Karlíčková, Ph.D. Title of Thesis: Assessment of chelation of cupric ions with flavones by use of hematoxylin method Copper is an essential trace element involved in many physiological processes. It is a part of several enzymes. Disruption of copper homeostasis results in pathological changes and diseases, excess of copper is highly toxic for human body. Flavonoids, the ubiquitous plant secondary metabolites, are highly abundant in the human diet. They represent a large group of polyphenolic compounds. They are characterized by a broad spectrum of biological activities. The anti-inflammatory, antiallergic, antimicrobial, hepatoprotective, immunomodulatory, cytotoxic and anticancer effects of flavonoids are well documented. Flavones belong to the group of flavonoids, they have chelating and antioxidative properties. They are known for their anti-inflammatory, antibacterial, antiviral and antiallergic activity. This thesis deals with chelating activity of six selected flavones, substituted on the A ring, namely 5-hydroxyflavone, baicalein, baicalin, chrysin, mosloflavone and negletein. Individual analyses were preformed using...
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Histological and histochemical methods used in study of development of internal male sex organs
VÁVROVÁ, Martina
Presented thesis devotes to comparative study of usability of fixation agents and colouring methods for the study of histology and histochemistry of Culex pipiens s.l. mosquito males. The study was carried out in males of Culex pipiens quinquefasciatus Say, which is a significant transmitter of filariasis. Histological and histochemistry studies of the structure of the whole abdomens or prepared testicles were carried out on the material fixed by fixation agents Carnoy and Bouin of Dubosque-Brasil modification. Fixed tissue was cast in paraplast or resin. Histological sections were done by means of automatic microtome Leica and half-thin sections by means of ultra microtone Reichert. Section preparations were coloured by Mayer or Harris haematoxylin or by colouring method according to Mallory. Semi-thin sections from materials cast in resin were coloured by toluidine blue. Fixation agent Bouin, Dubosque-Brasil modification proved as more convenient than Carnoy, either for the preparation of paraffin sections or for fabrication of half-thin sections from tissues cast in resin. The next advantage of this fixation is that fixed tissue can be stored for unlimited period of time without over fixation of the tissue as it is in the case of fixation by Carnoy. For general histological preparations Mallory colouring is apparently the most convenient from the used methods; it provides better orientation in coloured tissue thanks to the whole spectrum of colours-pink to red colouring of cell nuclei, blue colouring of tissue and secretions. In case of colouring of male genital organs all used colourings (Mallory, Harris and Mayer haematoxylin) seem to be comparable. Casting in resin with connection to colouring by toluidine blue (also histochemistry proof for carbohydrates) has shown to be suitable for visualisation of finer structures, in male genital organs particular developmental phases of male sex cells were distinguished.
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