National Repository of Grey Literature 7 records found  Search took 0.00 seconds. 
Biological activity evaluation of substances of natural origin II.
Kosinová, Nikola ; Šafratová, Marcela (advisor) ; Lomozová, Zuzana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy and Pharmaceutical botany Student: Nikola Kosinová Head of diploma thesis: PharmDr. Marcela Šafratová, Ph.D. Consultant: PharmDr. Jiří Janoušek, Ph.D. Title of diploma thesis: Biological activity evaluation of substances of natural origin II. In this thesis, I focused on studying the biological activity of the tested alkaloids isolated from the bark of the Geissospermum vellosii (Apocynaceae) stem. G. vellosii is a South American tree belonging to the genus Geissospermum Allemão, which is important in terms of the presence of active substances (mainly indole alkaloids). In traditional medicine, the active substances of this genus have been used for many years to treat malaria, tumors, bacterial infections, and pain. The isolated indole alkaloids were studied for their antineoplastic, anticholinesterase, analgesic, antimalarial, and anti-inflammatory activities. Some of the isolated alkaloids have significant biological activity, and it was the anticholinesterase activity that became the subject of this thesis. The significant anticholinesterase activity of the tested alkaloids represents a promising source for the development of new drugs for the treatment of Alzheimer's disease. The tested alkaloids were evaluated...
Evaluation of marine alkaloid fascaplysin as a potential antithrombotic agent
Kolářová, Zuzana ; Carazo Fernández, Alejandro (advisor) ; Lomozová, Zuzana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Zuzana Kolářová Supervisor: PharmDr. Alejandro Carazo, Ph.D. Title of the diploma thesis: Evaluation of marine alkaloid fascaplysin as a potential antithrombotic agent Fascaplysin, a pentacyclic indole alkaloid isolated from marine sponge Fascaplysinopsis bergquist sp., is one of the agents of marine origin that is receiving increasing attention and for which significant advances have been made. Fascaplysin is being investigated for, among other things, antineoplastic properties, where fascaplysin shows promising biological effect as a kinase inhibitor. In this study, the potential antithrombotic properties of this substance, which have not yet been sufficiently investigated, were analysed. For this purpose, plasma from 10 healthy donors was used. Aggregation experiments were performed on an optical aggregometer using platelet-rich plasma prepared from blood. Collagen or arachidonic acid (AK) was used to induce aggregation with or without a pre-incubation with fascaplysin at various concentration ranges. Fascaplysin significantly inhibited platelet aggregation for both agents tested. Collagen induction was inhibited at a significantly lower concentration than AK. Fascaplysin also was not...
The impact of small phenolic metabolites of flavonoids (propionic acids) on the iron-catalysed Fenton reaction
Spiller, Sára ; Lomozová, Zuzana (advisor) ; Karlíčková, Jana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacognosis and Pharmaceutical Botany Candidate: Sára Spiller Supervisor: PharmDr. Zuzana Lomozová, Ph.D. Title of diploma thesis: The impact of small phenolic metabolites of flavonoids (propionic acids) on the iron-catalysed Fenton reaction Keywords: iron, flavonoid, Fenton reaction, antioxidant, pro-oxidant Iron is an essential element, necessary for the proper functioning of the human body. It is involved in many cellular processes where it acts as a cofactor. It is physiologically very important, especially for its ability to readily accept and deliver electrons. Its amount in the body is strictly regulated because in excess of the free metal, harmful radical reactions and the development of oxidative stress could result. Oxidative stress is responsible for many pathological processes. Flavonoids are natural polyphenolic substances that are found in many plants. Their consumption is associated with beneficial effects on human health, including antioxidant, anti-inflammatory, and cardioprotective properties. Despite their beneficial effects, they may, under certain circumstances, possess pro-oxidant effects related to their redox properties. During the metabolism of flavonoids, the gut microbiome degrades them to simple...
The influence of benzoic acids (metabolites of flavonoids) on the copper-triggered Fenton reaction and hemolysis
Harčárová, Patrícia ; Lomozová, Zuzana (advisor) ; Karlíčková, Jana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacognosis and Pharmaceutical Botany Student: Patrícia Harčárová Supervisor: Zuzana Lomozová, MSc., Ph.D. Title of diploma thesis: Effect of benzoic acids (flavonoid metabolites) on copper catalyzed Fenton reaction and hemolysis Keywords: flavonoids, benzoic acids, copper, Fenton reaction, hemolysis Copper is a biogenic trace element that is present in several enzymes and proteins in the human body. The ability of copper to change its oxidation state is essential for its biological functions and enables enzymatic redox reactions in the organism. Homeostasis of copper is strictly regulated in the human body as its disruption can lead to the toxic effects of copper. Flavonoids are secondary plant metabolites that are a common part of our diet and their consumption has positive effects on our bodies. However, under certain conditions, they can also have a pro-oxidative effect, which is related to their ability to reduce transition metal ions. Flavonoids are massively metabolized in the human body before reaching systemic circulation into small phenolic substances including benzoic acids. Seven benzoic acid derivatives were tested as part of this thesis. Their influence on the production of hydroxyl radicals arising during the...
Interactions of natural phenolic compounds with biogenic metals
Lomozová, Zuzana ; Mladěnka, Přemysl (advisor) ; Bittner Fialová, Silvia (referee) ; Patočka, Jiří (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy and Pharmaceutical Botany Candidate: Zuzana Lomozová, MSc. Supervisor: Prof. Přemysl Mladěnka, PharmD., Ph.D. Title of Doctoral Thesis: Interactions of natural phenolic compounds with biogenic metals Phenolic compounds are one of the most widely distributed groups of secondary plant metabolites. They are an integral part of the human diet and their consumption is associated with a number of positive effects. They include a wide range of substances, from simple molecules such as phenolic acids to large polymeric compounds such as tannins. Because of their structure, which includes, among others, free hydroxyl groups, they are able to interact with biogenic metals and form metal complexes with them. Under normal conditions, the homeostasis of these transition metals in the body is tightly regulated, but it can be disturbed in pathological conditions such as acute myocardial infarction, when the levels of free iron and copper increase as a result of a significant decrease in pH. Similar metal imbalance is observed as well as in inflammatory and neurodegenerative diseases, tumours, or diabetes mellitus. The aim of this dissertation was to find out how these substances react with biogenic metals using in vitro and ex...
Interactions of natural phenolic compounds with biogenic metals
Lomozová, Zuzana ; Mladěnka, Přemysl (advisor) ; Bittner Fialová, Silvia (referee) ; Patočka, Jiří (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy and Pharmaceutical Botany Candidate: Zuzana Lomozová, MSc. Supervisor: Prof. Přemysl Mladěnka, PharmD., Ph.D. Title of Doctoral Thesis: Interactions of natural phenolic compounds with biogenic metals Phenolic compounds are one of the most widely distributed groups of secondary plant metabolites. They are an integral part of the human diet and their consumption is associated with a number of positive effects. They include a wide range of substances, from simple molecules such as phenolic acids to large polymeric compounds such as tannins. Because of their structure, which includes, among others, free hydroxyl groups, they are able to interact with biogenic metals and form metal complexes with them. Under normal conditions, the homeostasis of these transition metals in the body is tightly regulated, but it can be disturbed in pathological conditions such as acute myocardial infarction, when the levels of free iron and copper increase as a result of a significant decrease in pH. Similar metal imbalance is observed as well as in inflammatory and neurodegenerative diseases, tumours, or diabetes mellitus. The aim of this dissertation was to find out how these substances react with biogenic metals using in vitro and ex...
Interactions of tamarixetin and isorhamnetin with copper
Lomozová, Zuzana ; Karlíčková, Jana (advisor) ; Chocholouš, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany Candidate: Zuzana Lomozová Supervisor: PharmDr. Jana Karlíčková, Ph.D. Title of Thesis: Interactions of tamarixetin and isorhamnetin with copper Keywords: tamarixetin, isorhamnetin, chelation, reduction, copper, bathocuproine, hematoxylin Copper is a biogenic trace element important for proper function of human organism. It is an essential part of several enzymes and is involved in metabolic processes in the body. Excess or lack of serum copper can lead to pathological conditions. Copper chelating agents are used to treat copper toxic effects. Flavonoids are polyphenolic substances belonging to secondary metabolites of various plants. They are part of the human diet and have a positive impact on our health. They exhibit antioxidant and anti-inflammatory effects and are able to chelate transient metals, especially iron and copper. Chelation therapy is currently used in Wilson's disease in which copper is overloaded. In the future, the chelating effects of flavonoids could be used to treat neurodegenerative diseases, cardiovascular diseases or cancer. In this diploma thesis, interactions of two flavonoids (namely tamarixetin and isorhamnetin) with copper ions in different buffers were tested....

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