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Evolutionary dynamics of the CD45 molecule
Dlugošová, Sylvie ; Vinkler, Michal (advisor) ; Aghová, Tatiana (referee)
Determining the health state and condition of vertebrates based on hematological traits is currently dependent on technically imperfect and erroneous quantification of blood cells. Solution of this problem can be found in the use of flow cytometry. This is, however, complicated by the presence of nuclear erythrocytes in all vertebrate groups outside the mammals. To distinguish erythrocytes from leukocytes, it is necessary to use specific labeling with a leukocyte marker, which is the CD45 surface molekule encoded by the PTPRC gene. Current literature suggests high expression and structural interspecific variability of this molecule, which highly complicates the possible use of this marker in zoological research. Based on the prediction of domains from genomic sequence data, my analysis comparing the structure of the CD45 in various vertebrate representatives showed a high variability of the extracellular part, even in fibronectin domains, which have been earlier described as conservative. Although the CD45 receptor function has not been sufficiently resolved yet, the existing research results indicate strong positive selection aimed to this molecule. Taken together, although the CD45 is a useful molecular marker, the possibility of designing a universal antibody allowing testing of a wide range of...
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Assessment of cognitive impairment in schizophrenic patients
Kuhnová, Eva ; Hrachovinová, Tamara (advisor) ; Loneková, Katarína (referee)
The thesis deals with the contemporary topic of cognitive deficit present by schizophrenic people. It introduces the subjective point of view of the patients in more detail, which means the experience of deficits in the cognitive area. The text itself consists, as usual, of the theoretical and the research section. The theoretical section deals with the specifics of cognitive functions and their deficits present by schizophrenic patients. It focuses more closely on the subjective point of view of an individual, the impairment-related experience which follows from the impaired cognitive functions. The thesis pursues also the impact of cognitive impairment on an individual's life. A part of the theoretical section is also dedicated to the tools designated to measure cognitive functions. The major subpart is dedicated to self-evaluating tool entitled Frankfurt Complaint Questionnaire (FCQ), concerning primarily its origin, description and psychometric properties. The research section is based on the usage of the Czech translation of the FCQ in practice. Based on an observation of a sample of 53 individuals diagnosed with F20.0 and 53 healthy people, the psychometric qualities of the FCQ (discriminant validity, Cronbach's alpha, test-retest reliability) were examined. In case of patients diagnosed with...
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Assessment of diarrheal problems by calves on selected dairy cattle farm.
POLÁČKOVÁ, Eva
The aim of this thesis was to evaluate the prevalence of diarrheal diseases in calves depending on the level of postnatal care of newborns on the selective dairy cattle farm. A total of 299 calving was evaluated on the farm in the period 2013 - 2015. The total mortality of calves was less than 7 %, in which mortality due to diarrheal diseases represented less than 3 %. The colostrum quality was assessed as excellent in 74%. Passive immunization, determined as the total protein content in the blood of calves, was performed in 72 % of the calves well. 60 % of calving happened in ideal hygienic conditions and diarrhea was found in 25 % of them. In contrast, diarrheal diseases were found in 65 % of calving in poor hygienic conditions.
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Comparing the impact of housing and milking technologies on selected indicators of performance and quality of milk
POLÁKOVÁ, Radka
The theme of this thesis is to compare free housing with a milking robot, milking of serious housing in the pipeline. The evaluation is focused on milk yield and its quality, reproductive performance and health. It is also monitored by the free choice of milking cows during the day. Consideration is also observed in stable microclimate. Everything is assessed under operational conditions. Object of study is a refurbished barn, where both systems are in operation. Were measured and compared observed values of milk production, reproduction and health status between groups. The observed values were evaluated in the program Statistica 9th of a computerized milking robot system was speciaed the period of time when Nada dairy milk provides the most of milk through a robot.. In the area of dairy cattle milking robot reaches a higher milk yield and increasing the percentage of lactose recovery. Milking stalls of the pipeline has better results in the percentage of protein recovery. The choice of time period of milking cows, the most milk by dairy cows wasprovided between 10:00-18:00 pm. In the area of reproduction achieved better results with loose housing milking robot. The issue of health status showed a statistically better health outcomes for the free housing with a milking robot.
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Accompanying of Dying People in Retirements Homes Including the Elements of Hospice Care
VALÁŠKOVÁ, Michaela
The thesis deals with accompanying of dying people in retirements homes including the elements of hospice care. First, the difference between mission of retirements homes and hospices is described. Based on theoretical pieces of knowledge from literature, there are defined: the dying, the reaction of a dying man to the message about his health state, needs of a dying man, care for the family of a dying man, engagement of a multidisciplinal team, death and care for surviving relatives. In the second half, the research on internal rules is described. The research is aimed at findings, if and how the single retirements homes treat the topic of accompanying of dying people in their internal rules. Last chapter results from the previous and offers the plan for creating the internal rules for accompanying of dying people.
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