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Monitoring of Phytoestrogens Expression to the Milk and Milk Products
Watzková, Jarmila ; Rittich, Bohuslav (referee) ; Doležal, Petr (referee) ; Omelková, Jiřina (advisor)
Phytoestrogens are very intensively studied in recent years because they show different pharmacological effects on human health. For example, it was demonstrated that phytoestrogens reduce the risk of certain cancers (breast, prostate or colon), act as antioxidants in the body and affect the activity of certain enzymes. In addition, also it was demonstrated number of positive effects of soybean phytoestrogens on the health status of women in the menopause, such as reducing the risk of atherosclerotic cardiovascular disease or osteoporosis. Various technologically processed soy beans are important part of the feed rations mainly for high-lactating dairy cows, because they are a valuable source of the energy and high-quality protein, despite their negative effect on reproductive performance. Phytoestrogens consumed from the feed rations are hydrolyzed in the rumen of dairy cow and together with their metabolites are absorbed in the small intestine into the blood. They can be excreted from the body in urine or milk. From this perspective, cow's milk may become a source of phytoestrogens substances, especially equol in the human nutrition. On the other hand, some research results suggest possible potential risks associated with phytoestrogens intake in the diet on human health. At the some age (infants and young children) and group (vegetarians) categories may be dietary intake of phytoestrogens undiserable. According to most experts, a beneficial effect on human health prevail the potential risks. However, the risks associated with phytoestrogens intake in food can not be ignored and therefore phytoestrogens are still actual problem in the field of scientific research.
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Comparison of interactions of equol and desmethylangolensin with iron and copper
Němcová, Hana ; Karlíčková, Jana (advisor) ; Matysová, Ludmila (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany Candidate: Hana Němcová Supervisor: PharmDr. Jana Karlíčková, Ph.D. Title of Thesis: Comparison of interactions of equol and desmethylangolensin with iron and copper Iron and copper are essential trace elements, which are important for our body. Both elements have a significant effect on the correct function of organs and make part of many enzymes. They are able to accept or donate electrons - conversion between oxidized (Fe3+, Cu2+) and reduced (Fe2+, Cu+) forms. If these metals are excess in organism, they are accumulated in the cells and mediate the creation of free radicals, that destroy cell structures. This deficiency is treated with chelators, which facilitate the excretion of metals from the body. Isoflavonoids are polyfenolic substances, which can have antioxidant effects and they are involved in the scavening of free radicals. Isoflavonoids can have also a pro-oxidative effect, because they are able to reduce metal ions. In this study were tested interactions (chelation and reduction) between the metabolites of isoflavonoids (equol and desmethylangolensin) and ions of iron and copper. Both metabolites only weakly chelate metal ions, but they significantly reduce cupric ions. KEYWORDS: Iron,...
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Koncentrace vybraných fytoestrogenů v krmné dávce dojeného skotu a jejich distribuce do krve a mléka
Bařinová, Michaela
Diploma thesis on the theme Concentration of selected phytoestrogens in the diet of dairy cattle and their distribution in to the blood and milk deals about transmittance of phytoestrogens from real fed TMR in the South Moravian conventional breedings of dairy cows into the blood plasma of animals and to milk as a product intended for hu-man consumption. A review of literature is devoted to the occurrence of phytoestrogens in forage and feed, their metabolism and effects on animals and humans. The experimental part of the thesis is devoted to the identification and quantification of selected phytoestrogens in feed and their penetration into blood plasma and milk. Determination of phytoestrogens was performed by HPLC - MS analysis.
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Fytoestrogeny v potravinářství
Došková, Markéta
Phytoestroegens are biologically active compounds naturally occuring in plant materials. This group consists mainly of polyphenols characterized by structural similarities with female hormone 17-beta-estradiol. Plants produce phytoestrogens as their secundary metabolities, primarily due to the reaction as a response to various stress. Agents with estrogen effect contained in foodstuffs bring very positive impact on our health. On the other hand, some of the estrogen compounds may be harmful to the health depending on the concentration and the complex state of the consumer, as well as mycoestrogens, xenoestrogens and antiestrogens. Also for this reason is necessary to detect and quantify them in the sample of food. The major problem encountered in the studies of their effect is large amount of a variety of other substances in biological material, and according to their complicated structure was important to establish a reliable method, that was used also in the practical part of my experiment. It is an UPLC-MS/MS -- combination of Ultra High Performance Liquid Chromatography and Tandem Mass Spectrometry preceded by complex sample preparation which involves also SPE purification ("solid phase extraction").
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Monitoring of Phytoestrogens Expression to the Milk and Milk Products
Watzková, Jarmila ; Rittich, Bohuslav (referee) ; Doležal, Petr (referee) ; Omelková, Jiřina (advisor)
Phytoestrogens are very intensively studied in recent years because they show different pharmacological effects on human health. For example, it was demonstrated that phytoestrogens reduce the risk of certain cancers (breast, prostate or colon), act as antioxidants in the body and affect the activity of certain enzymes. In addition, also it was demonstrated number of positive effects of soybean phytoestrogens on the health status of women in the menopause, such as reducing the risk of atherosclerotic cardiovascular disease or osteoporosis. Various technologically processed soy beans are important part of the feed rations mainly for high-lactating dairy cows, because they are a valuable source of the energy and high-quality protein, despite their negative effect on reproductive performance. Phytoestrogens consumed from the feed rations are hydrolyzed in the rumen of dairy cow and together with their metabolites are absorbed in the small intestine into the blood. They can be excreted from the body in urine or milk. From this perspective, cow's milk may become a source of phytoestrogens substances, especially equol in the human nutrition. On the other hand, some research results suggest possible potential risks associated with phytoestrogens intake in the diet on human health. At the some age (infants and young children) and group (vegetarians) categories may be dietary intake of phytoestrogens undiserable. According to most experts, a beneficial effect on human health prevail the potential risks. However, the risks associated with phytoestrogens intake in food can not be ignored and therefore phytoestrogens are still actual problem in the field of scientific research.
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