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Optimization of a Flow Rate for a Hyphenation of Voltammetry with Electrospray Ionization Mass Spectrometry
Jaklová Dytrtová, Jana ; Jakl, M. ; Norková, Renáta ; Navrátil, Tomáš
The hyphenation of an electrochemical cell prior to mass spectrometer with electrospray ionization allows studying of products and/or of intermediates of electrode reactions. The measurement is realized in a flowing system. The flow rate of the sample markedly influences the MS signal intensity of the product. The calculated optimum value for the electrochemical cell with the sweep volume 0.72 μL is in the range from 0.4 to 0.5 mL h -1 . The experimental optimal value is 0.45 mL h -1 . The optimization of the flow rate has to be provided individually for each product, because the intensity also depends on the rate of the electrode reaction and product stability.
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Artificial phospholipid membranes as models of real membranes and transport of charged particles across them
Parisová, Martina ; Navrátil, Tomáš ; Šestáková, Ivana ; Kohlíková, E. ; Petr, M.
Because the real bio-membranes and the tranporting processes realized across them are very complicated, the model membranes have been used for elucidation of the basic transporting steps. In the contribution, the different possibilities of characterization, description, and elucidation of the transport processes on the example of some metal ions and theor complexes, i.e., of cadmium, lead, and copper, were presented. It summarizes the results achieved using supported phospholipid model membranes formed on porous membranes and on agar support. Discussed transporting processes of selected ions have been characterized using two electrochemical methods: electrochemical impendance spectrometry (EIS) and voltammetry. The impact of some parameters on these systems and processes was investigated.
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Tail labelled oligonucleotide probes for the detection of DNA-protein interactions
Pivoňková, Hana ; Němcová, Kateřina ; Horáková Brázdilová, Petra ; Havran, Luděk ; Macíčková-Cahová, Hana ; Hocek, Michal ; Fojta, Miroslav
DNA-protein interactions can be monitored via different ways. We introduce novel, fast and simple approaches in DNA-protein interaction detection based on electrochemical measurements of DNA alone (structure-sensitive DNA sensing), or DNA modified with osmium tetroxide bearing nitrogenous ligands, or measurements of redox-active moieties enzymatically attached to the end of a DNA substrate thus forming a labeled tail (by terminal transferase).
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Redox labelling of nucleic acids for analyzing nucleotide sequences and monitoring DNA-protein interactions
Fojta, Miroslav ; Havran, Luděk ; Horáková Brázdilová, Petra ; Pivoňková, Hana ; Kostečka, Pavel ; Macíčková-Cahová, Hana ; Raindlová, Veronika ; Vrábel, Milan ; Hocek, Michal
Nucleobase labelling of DNA for electrochemical sensing was attained through chemical modification of thymine bases with osmium tetroxide in the presence of nitrogenous ligands, or via enzymatic incorporation of nucleotide conjugates with redox-active moieties using labelled deoxynucleoside triphosphates. DNA hybridization, primer extension and PCR techniques were used for sequence-specific DNA assays. Tail-labelled DNA substrates were applied to monitor DNA binding by tumour suppressor p53 protein.
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