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Comparison of sensitivity of Bacillus subtilis and Clostridium difficile against selected chemical ingredients.
Šlitrová, Barbora ; Ing.Veronika Holá, Ph.D. (referee) ; Omelková, Jiřina (advisor)
Sporicidal activity of disinfectants is tested on Bacillus subtilis spores according to ČSN EN 13704 standard. Until December 2007, 10 countries encountered outbreaks and 7 additional countries had endemic cases of Clostridium difficile-associated disease due to hypervirulent strain C. difficile PCR ribotype 027. This outbreak of CDAD with increased severity and mortality is spread as typical nosocomial infection. Clostridium difficile spores can persist on dry surfaces for 5 months. Therefore it is important to compare the effectiveness of disinfectants on both strains and find out whether Bacillus subtilis is the only proper microorganism for testing the sporicidal activity.
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Comparison of the biocidal activity of disinfectants against biofilm
Šlitrová, Barbora ; Votava,, Miroslav (referee) ; Omelková, Jiřina (advisor)
A common vector of nosocomial infections is Pseudomonas aeruginosa. This opportunistic pathogen is resistant against different classes of antibiotics as well as against biocides. Some P. aeruginosa strains are able to form biofilm. The biofilm formation on medical devices represent a common occurrence that can lead to serious illness and death. Bacteria in biofilms are more resistant than their planktonic counterparts. Therefore it is important to find disinfectants that will be effective also against biofilm. Bactericidal efficacy of four disinfectants has been evaluated on biofilm and on planktonic forms of P. aeruginosa.
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Selection of suitable disinfections to prevent the spread of Corynebacterium pseudotuberculosis in farms focused on the production of milk products and meat
Liová, Alexandra ; Pořízka, Jaromír (referee) ; Marková,, Jiřina (advisor)
This diploma thesis deals with the selection of suitable disinfectants and their effective concentrations for the prevention of the spread of the causative agent of caseous lymphadenitis in sheep and goat farms with a focus on milk processing, dairy and cheese production or meat production. For these purposes, disinfectants were selected from the group of quaternary ammonium compounds, oxidizing compounds, biguanides and alcohols, which were tested according to the ČSN EN 1656 standard on Corynebacterium pseudotuberculosis isolates forming a biofilm. The theoretical part first deals with the general characterization of the bacterial species Corynebacterium pseudotuberculosis and caseous lymphadenitis. In other chapters, the bacterial biofilm, its formation and the factors that influence it are described. Last but not least, the theoretical part is focused not only on the legislative treatment of the use or testing of disinfectants, but also on the closer characterization of their individual groups. In the experimental part, the procedure for determining the sensitivity of Corynebacterium pseudotuberculosis biofilms to selected disinfectants (benzalkonium chloride, chlorhexidine digluconate, ethanol, iodinated povidone, sodium hypochlorite and peracetic acid) was first optimized according to the ČSN EN 1656 standard. The optimization was followed by the cultivation and formation of biofilms of Corynebacterium pseudotuberculosis and the evaluation of their intensity by spectrophotometric determination of optical density. Subsequently, tested disinfectants were applied to the obtained biofilms, and after 24 hours of incubation from the end of the disinfection process, the effectiveness of the tested disinfectants was visually evaluated. At the end of the research, the minimum bactericidal concentrations for individual disinfectants were determined and the concentrations of these substances suitable for preventing and preventing the spread of the bacterium Corynebacterium pseudotuberculosis in small ruminant farms were also selected.
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Postup dezinfekce a čištění textilních závěsů pečetí
Drábková, Klára ; Škrdlantová, Markéta ; Nagyová, Dominika ; Krejčí, Jan ; Bacílková, Bronislava ; Bureš Víchová, Jana ; Ďurovič, Michal
Textilní závěsy jsou nedílnou součástí archivních dokumentů s přivěšenou pečetí a jejich udržení v dobrém stavu je podmínkou jak pro uchování pečetě, tak i právní hodnoty listiny. Proto je cílem této metodiky doporučit vhodný a bezpečný postup dezinfekce a čištění textilních závěsů pečetí. Konzervování textilních závěsů je specifické kvůli jejich kontaktu s dalšími materiály archiválií (pečeť, papír, pergamen apod.) a není tedy možné využít všechny metody běžně užívané v procesu konzervování-restaurování textilií. Přínosem této metodiky je zohlednění výše uvedených specifik. Textilní závěsy pečetí, mohou být znečištěny jak prachovými nečistotami, tak nečistotami vzniklými při výrobě pečetí, či předchozím restaurátorským zásahem, případně mohou být mikrobiologicky napadeny. Vhodná dezinfekce a dostatečné vyčištění textilního závěsu přispějí k jeho dlouhodobé stabilitě.
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Hand hygiene of healthcare workers providing care in a medical facility
Hájková, Iveta ; Kordulová, Pavla (advisor) ; Kulířová, Veronika (referee)
The subject of the bachelor thesis is focused on issues related to hand hygiene in health care workers, in the provision of care in the health facility. Even though this topic is often mentioned, it is in practice less understood or misinterpreted. Sometimes, it is neglected or downplayed by health workers. The theoretical part is divided into 7 chapters. Here is anatomy and physiology of the skin, microbiological and epidemiological situation, measures aimed at eliminating the source and avoiding the route of transmission, effective methods of disinfection, sterilization, elimination of microorganisms and their transmission, in the health facility. Hand hygiene and its techniques, proper use of personal protective equipment, nail hygiene and, last but not least, skin care. Explaining the concept of hospital infection, which increases morbidity and mortality of patients, increases both diagnostic and therapeutic interventions and generally aggravates the quality of care provided with prolonging the period of hospitalization. I have worked on a search for relevant resources on the subject. The sources I drew mainly from WHO guidelines, CDC, Decree No. 306/2012 Coll. and on the basis of a literature search from the National Medical Library. I received the consent to perform the action research from...
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Comparison of the biocidal activity of disinfectants against biofilm
Šlitrová, Barbora ; Votava,, Miroslav (referee) ; Omelková, Jiřina (advisor)
A common vector of nosocomial infections is Pseudomonas aeruginosa. This opportunistic pathogen is resistant against different classes of antibiotics as well as against biocides. Some P. aeruginosa strains are able to form biofilm. The biofilm formation on medical devices represent a common occurrence that can lead to serious illness and death. Bacteria in biofilms are more resistant than their planktonic counterparts. Therefore it is important to find disinfectants that will be effective also against biofilm. Bactericidal efficacy of four disinfectants has been evaluated on biofilm and on planktonic forms of P. aeruginosa.
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Comparison of sensitivity of Bacillus subtilis and Clostridium difficile against selected chemical ingredients.
Šlitrová, Barbora ; Ing.Veronika Holá, Ph.D. (referee) ; Omelková, Jiřina (advisor)
Sporicidal activity of disinfectants is tested on Bacillus subtilis spores according to ČSN EN 13704 standard. Until December 2007, 10 countries encountered outbreaks and 7 additional countries had endemic cases of Clostridium difficile-associated disease due to hypervirulent strain C. difficile PCR ribotype 027. This outbreak of CDAD with increased severity and mortality is spread as typical nosocomial infection. Clostridium difficile spores can persist on dry surfaces for 5 months. Therefore it is important to compare the effectiveness of disinfectants on both strains and find out whether Bacillus subtilis is the only proper microorganism for testing the sporicidal activity.
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