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Mladý ječmen a chlorella jako součást zdravého stravování
BÍCOVÁ, Pavla
Products of young barley and chlorella belong to the increasingly popular nutraceuticals due to their rich nutritional composition. The aim of the thesis was: to assess the offer of young barley and chlorella in the market network in the Czech Republic, to evaluate the knowledge about these products and the effects of using these dietary supplements by questionnaire survey (n = 338) and to assess the quality of the selected products. There is a relatively wide range of chlorella (57 products; 75 % in tablet form) and young barley (24 products; 71 % in powder form) products on the Czech market, which differ in composition, processing method, country of origin, price and information provided about them. Knowledge of the production method and the contents is important when consumers choose products. This knowledge was found to be low among some users. Awareness increased statistically significantly with age and educational attainment. The majority of users of chlorella (181; 55 %) and young barley (224; 68 %) did not suffer from side effects associated with initiation of use. The most frequently reported adverse symptoms were related to the gastrointestinal tract (diarrhoea, constipation). The most frequently reported beneficial effects were more energy and vitality, improved digestion and immunity. The microbial quality of the chlorella and young barley samples met the legislative requirements. Total polyphenol content and total antioxidant activity were lower (1.20?0.00 mg/g - 1.35?0.01 mg/g, 0.73?0.00 mg/g - 0.84?0.02 mg/g, respectively) in chlorella (11.44?0.26 mg/g - 7.92?0.25 mg/g, 10.36?0.00 mg/g - 22.76?0.27 mg/g, respectively) compared to young barley.
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Recovery of waste from processing of phosphorus to produce materials with increased added value
Hejsek, Michal ; Zavřel, Tomáš (referee) ; Ing.Jan Červený, Ph.D. (advisor)
This master thesis focuses on reuse of waste water from industrial plant processing yellow phosphorus. Theoretical part summarizes physical and chemical properties of phosphorus, its transport in biosphere and its role in living organisms. Also the production process of the industrial plant is described. Waste water that contains waste byproducts is considered to be useful source of essential nutrients for economical large scale microalgae cultivation and development of biotechnological processes. In practical part, optimization of growth conditions for microalgae Chlorella pyrenoidosa Chick (IPPAS C-2) cultivation in medium based on wastewater from yellow phosphorus warehouse is presented.
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Use of autotrophic algae to production of biomass and some metabolites
Chrástová, Nikola ; Kostovová, Iveta (referee) ; Márová, Ivana (advisor)
Chlorophyll and carotenoids are natural pigments and antioxidants. These pigments are suitable for use in the pharmaceutical, cosmetics or food industries. Lipids are another major metabolite of algae and are a potential feedstock for biofuel production. This bachelor thesis deals with the cultivation of selected genera of autotrophic algae, subsequent isolation of these selected metabolites and their application. The theoretical part focuses on the description of the algae, the metabolites produced, the cultivation and the chosen methods. The experimental part deals with the optimization of the cultivation of algae of the genera Chlorella, Chlamydomonas and Desmodesmus, depending on the chosen medium and intensity of illumination, determination and quantification of algal biomass. Further, extraction of lipidic and carotenoid substances and determination by HPLC and GC was performed. In the overall comparison, the best producer of biomass is the algal genus Desmodesmus and the highest production of pigments and lipid substances was reached in algae of the genus Chlamydomonas.
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Issues of the algae Chlorella production in flow bioreactors
Jankovičová, Kristína ; Lukavský,, Jaromír (referee) ; Svěrák, Tomáš (advisor)
Microalgae invite the attention of scientists due to their unique properties, including their quick growth, accumulation of lipids and other valuable substances, fixation of carbon dioxide and treatment of wastewater. This master´s thesis is focused on the study of microalgae. The main goal is to understand and describe the process of microalgae cultivation, in order to optimize it. The theoretical part of this thesis deals with microalgae (mainly Chlorella sp.) characterization, its practical use and cultivation optimization in order to obtain the highest concentration of biomass. The experimental part is divided into three tasks. Aim of the first task was the comparison of the course of autotrophic and heterotrophic cultivation of various strains of Chlorella and Coccomyxa microalgae, using three different cultivation media – synthetic medium for chlorella cultivation and natural fertilizer, Florium, used in two different concentrations (diluted 50 and 20 times). The highest Chlorella sp. biomass concentration of 7,10 g/l was achieved in the synthetic heterotrophic medium. Second task was focused on monitoring of the growth of algae Coccomyxa and Chlorella strain C1A, with respect to temperature and light intensity, using various combinations of these two important growth factors. Chlorella achieved its highest concentration of 11,46 g/l when grown at temperature of 33,5 °C and light intensity of 320 µE.m2.s1. The third and final task was to observe the growth of Dictiosphaerium chlerelloides microalgae on a flat cascade bioreactor. The experiment led to the discovery that these algae were able to grow at temperatures of around 10 °C, at which many well-known commercial algae, such as Chlorella sp. or Arthrospina sp., simply wouldn’t grow.
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Issues of the algae Chlorella production in flow bioreactors
Jankovičová, Kristína ; Kaštánek,, Petr (referee) ; Svěrák, Tomáš (advisor)
The bachelor's thesis is focused on cultivation of Chlorella microalgae species. Theoretical part deals with microalgae characteristics, their usage and methods of their cultivation. It is focused on the most important growth factors of chlorella and compares findings from earlier studies. Experimental part contains observations of microalgae growth in different environments and behavioural comparison of autotrophic and heterotrophic cultures. Next, the influence of temperature and light intensity on microalgae growth was observed, using different combinations of these factors. Experimental findings were evaluated from gained biomass concentration and absorbance determined by spectrophotometric method.
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Cultivation, Monitoring and Application of Migroalgae Cultures.
RANGLOVÁ, Karolína
This thesis deals with microalgae mass cultures grown in laboratory as well as outdoor cultivation systems and a potential use of their biomass. Case studies illustrate the correlation of changes in growth rate with photosynthetic activity, physiological features and biomass composition under various conditions (high irradiance intensity, optimal/suboptimal temperature and the presence of some metalloids). The special attention was also paid to evaluation of the bioavailability of Se-enriched Chlorella biomass which is commonly used as a food supplement. As concerns methodology, photosynthesis monitoring techniques, namely chlorophyll fluorescence were employed as an important tool to estimate microalgae activity.
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Impact of various selenium supplements offered sheep on lambs´vitality
HUPKOVÁ, Petra
The aim of my thesis was to compare the effects of selenium in organic form (bound to Chlorella algae and to yeast) and of selenium in inorganic form (Na2SeO3) on the growth and some chosen hematological and biochemical parameters. Two experiments were conducted. During the first one, the effect of selenium in organic form bound to Chlorella algae and selenium in inorganic form was observed when offered gravid sheep against a control group without selenium. During the second one, the effects of selenium in inorganic form and in various organic forms were monitored when offered the sheep after their delivery. The experiments proved positive impact of selenium offered the sheep already during the period of their gravidity (whether in organic or inorganic form) on the growth of the lambs and some chosen biochemical blood parameters of theirs. Selenium in organic form bound to Chlorella, the fluvial algae, had more positive effects on the lambs´ growth parameters than organic form bound to yeast and than inorganic form (Na2SeO3). The effect of selenium in organic form bound to yeast offered the sheep after their delivery was not better than offering inorganic sodium selenite. Lambs´ growth parameters seem to be better when the sheep are offered selenium already during their gravidity in comparison with the sheep having already delivered. The pure Chlorella added into the feeding ration for gravid sheep has a positive impact on lambs´ birth weight and growth parameters. The need appears to reconsider alcaline phosphatase values which the literature states as objective.
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Auxin transport in algae
Skokan, Roman ; Petrášek, Jan (advisor) ; Bíšová, Kateřina (referee)
Phytohormone auxin plays an important role in regulating plant development. Directional (polar) cell-to-cell auxin transport creates auxin gradients within plant tissues, which trigger a specific developmental response. The vast majority of available data concerns angiosperms. Lower land plants have been much less explored in this regard, but the important auxin-related mechanisms (including polar auxin transport) are already present in mosses. To uncover the origins of auxin action, one must focus on green algae, especially of clade Streptophyta, which are the direct ancestors of all land plants. In this study, the possible effects of auxins, both native and synthetic, were investigated on two algae: basal, unicellular Chlorella lobophora and advanced, filamentous Spirogyra sp. The latter received comparably more attention, since it belongs to a clade now acknowledged as a sister group to land plants. Chlorella lobophora culture growth was irresponsive to synthetic auxin NAA. The average Spirogyra sp. cell length was, however, changed by auxins at high concentrations. By conducting accumulation assays of radioactively labelled auxins and HPLC analysis, auxin metabolism and transport was investigated in Spirogyra sp. This alga was able to metabolize the plant-native IAA, but not synthetic auxins...
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