|
| |
|
|
The role of asparagine synthetase in leukemic cells
Šafrhansová, Lucie ; Starková, Júlia (advisor) ; Čuřík, Nikola (referee)
This thesis focuses on the detection of mutations in the enzyme asparagine synthetase on leukemia cell metabolism and the role of this enzyme in the context of L-asparaginase- based chemotherapy. The experimental part of the work is divided into two separate sections. Given the lack of asparagine synthetase gene sequencing data in leukemias, the first objective was to determine whether mutations are present in the leukemia cell line that could affect ASNS function and thus play a role in the resistance of leukemia cells to ASNase therapy. No mutations that could affect the activity of the enzyme were detected by next-generation sequencing. In the second part, a model of RS4;11 that expresses ASNS was established. The effect of ASNS on glycolysis was then studied to sensitize these cells to the effects of L-asparaginase and to the depletion of asparagine and glutamine. It was observed that ASNS expression increased the level of glycolysis and increased the resistance of these cells to asparagine and glutamine depletion and their resistance to asparaginase. Key words: ASNS, aspragine, leukemia, L-asparaginase, chemotherapy, drug resistance
|
|
|
Cardiotoxic effects of anthracyclines in oncological treatment
Tekáčová, Kateřina ; Horníková, Daniela (advisor) ; Vávra, Jiří (referee)
Anthracyclines are antitumor antibiotics used during chemotherapy in cancer patients. Treatment with anthracyclines has good results but a side effect is significant cardiotoxicity. This bachelor thesis describes the mechanisms of action of anthracyclines which are essential in cancer therapy and may be risky for patients. The thesis also defines the term of cardiotoxicity and summarizes factors that may increase the risk of cardiotoxicity. The most commonly used agents in the anthracycline series are briefly described. Cardiotoxicity can be detected by cardiac markers, especially particular troponin and diagnostic methods. The last chapter describes the groups of substances that reduce the cardiotoxic effects of anthracyclines. Key words: heart, anthracyclines, cardiotoxicity, chemotherapy, onkology treatment
|
|
|
Nutritional status of patients with acute leukemia during the oncological treatment
Kupilíková, Veronika ; Šťastná-Marková, Markéta (advisor) ; Křížová, Jarmila (referee)
Acute leukemia is defined as a heterogeneous group of malignant hematopoietic stem cell disorders. The aim of this study was to investigate the extent to which nutritional disorders occur in patients with acute leukemia during the cancer treatment, their severity, the most common causes of weight loss, and the extent to which different forms of nutritional intervention are applied in patients. The research was carried out in the Institute of Haematology and Blood Transfusion, during the hospitalization of patients in the inpatient ward, intensive care unit and transplant unit. The study population consisted of 40 patients diagnosed with acute leukemia. Outcomes from nutritional status screening (MNA questionnaire, BMI, weight, mid arm circumference, calf circumference, BIA, biochemical nutritional parameters), and functional assessment (ECOG PS, Chair-stand test, Hand grip test) were used to process the practical part and answer the research questions. Measurements were performed twice with approximately one month intervals. The study confirmed weight loss since the onset of the disease in 95 % of patients. The dominant cause of weight loss was identified in 80 % as lack of appetite. Nutritional screening by MNA evaluated the patient population with a mean score of 22,8 ± 2,9, referring to the risk...
|
|
|
Diet at patients after total pancreatectomy
ŠEDIVÁ, Denisa
In the bachelor degree project Diet for the patients after total pancreatectomy I deal with a diet of the patients who underwent surgery called total pancreatectomy. The introduction of the theoretical part is focused on the anatomy of the pancreas and its function. The following part of the project is directly focused on carcinoma, its epidemiology, incidence, cause of the disease and also development. I also deal with clinical symptoms, treatment and pancreatectomy itself. The last part of the project is oriented towards nutrition for the patients after surgery. Further in this section, I deal with pancreatic and diabetic diet and diet during chemotherapy, which many of the respondents have undergone. As the goal of the project, I chose to look into the alimentation of the patients with this disease and to find out how difficult it is to combine three types of diet together. I focused on the observation of the intake of basic nutrients such as carbohydrates, fats, and proteins. The practical part I compose as a qualitative research. There were 6 respondents who took part in the research. Unfortunately, only two of them provided me with their blood glucose values to evaluate their alimentation according to the diabetic diet. The result was very surprising. In relation to the seriousness of the illness, most of them ate basically without restriction. They were eating everything they wanted except sweet dishes, for example cakes and fried dishes. They just watched their blood glucose values. Despite all the experienced weight loss, their current alimentation meets recommendation and no one of them suffers from lack of any of the macronutrients according to the results.
|
|
|
Immunomodulatory effect of tumor targeted polymer drugs
Mervartová, Ivana ; Šírová, Milada (advisor) ; Palich Fučíková, Jitka (referee)
5 Abstract Myeloid-derived suppressor cells (MDSC) are a very heterogeneous population of immature, activated myeloid progenitors of neutrophils, monocytes/macrophages and dendritic cells that have not differentiated into mature forms. A common feature of these cells is the ability to suppress immune responses of T cells, NK cells, and dendritic cells. It is known that MDSC accumulate under various pathological conditions, such as chronic inflammation or cancer. In breast cancer patients, the highest MDSC counts correlate with the occurrence of metastatic foci in lung tissue. The suppressive effects of MDSCs are associated with resistance to chemotherapy, reduced effectiveness of immunotherapy and overall poor prognosis of the disease. Therefore, many studies focus on MDSC. One possibility is the differentiation of MDSC into mature populations that lose their suppressive phenotype. In this work, we focused on modulation of MDSC activity by all-trans retinoic acid (ATRA), bound to a polymer conjugate based on N-(2-hydroxypropyl)methacrylamide (HPMA). ATRA is used in clinical practice for the treatment of acute promyelocytic leukemia, where the mechanism of action is the differentiation of pathological cells into more mature forms and thus the cessation of their proliferation. The binding of ATRA to the...
|
|
|
NURSING CARE OF CANCER PATIENTS WITH HAEMATOPOIESIS DISORDER IN CONNECTION WITH ANTITUMOR CHEMOTHERAPY
BENEŠOVÁ, Petra
Abstract The aim of this thesis was to find the specifics of nursing care of cancer patients with haematopoiesis disorder in connection with antitumor chemotherapy and to map out the compliance with the specific measures for haematopoiesis disorder as a result of chemotherapy. We also focused on complications which emerged with administering chemotherapy, patient education about regime measures and on patient evaluation of the nurses' nursing care and their cooperation. Methodology and research file: to meet the target, a qualitative research was used in the form of semi-structured interview with the nurses and patients. The results of the interviews were analysed with the help of open coding with the pencil-paper method and subsequently divided into categories with subcategories. For nurses 3 categories with 4 subcategories were created and for patients 5 categories with 2 subcategories. The first research file consisted of twelve nurses working in oncology departments of five hospitals in the Czech Republic. The second research unit consisted of seven patients chosen with deliberate selection method. The results: It was found that the nurse in the care of cancer patients with haematopoiesis disorder works as a provider of barrier nursing care. The nurse takes the role of an educator during the application of chemotherapy and during the discharge of the patient into home healthcare, when she informs the patient about the need to comply with the specific measures which are diet regime, limited contact only to immediate family, the unsuitability of visiting restaurants, specific habits and using respiratory protection. All of the patients agreed that they try to follow the recommended regime measures. Simultaneously, they evaluated the measures as limiting both in work and personal life. The results for practice: The result of this research can be a theme for creating a information brochure for cancer patients or a topic for an educational seminar for nurses discussing this issue.
|
|
|
Caring for blood cancer patient undergoing chemotherapy
BAIERLOVÁ, Angélique
The basic haemato-oncological diseases include leukaemia, malignant lymphomas, myeloproliferative diseases and myelodysplastic syndrome. The treatment of these diseases is most often carried out with the help of chemotherapy, which, in addition to its therapeutic effect, also has a number of side effects that may reduce the patient's quality of life. During treatment, the role of the nurse is important, ensuring the safe application of chemotherapy, caring for venous access, monitoring the side effects of treatment and participating in patient education. The aim of the bachelor thesis was to find out the specifics of nursing care of a haemato-oncological patient during chemotherapy. The practical part was processed using a qualitative research survey. The first research group consisted of six nurses working in an oncology centre or inpatient department. The second research group consisted of six haemato-oncological patients undergoing chemotherapy. The semi-structured interview became the technique of data collection. The results of the research show that the nurses treat long-term venous accesses insufficiently. Problems were also found in the treatment of extravasations and in the application of the cytostatic drug itself. The results also showed that nurses do not use the right protective equipment when handling cytostatics. Another negative finding was that patients did not have enough information about the prevention of the side effects of chemotherapy and did not have sufficient psychological support from the nurses. Nevertheless, they evaluate the provided care positively. Based on the results, an information brochure designed for haemato-oncological patients undergoing chemotherapeutic treatment was designed as part of the thesis.
|
|
| |
|
|
Vliv chemoterapie a genotoxického stresu na imunologické vlastnosti nádorových buněk.
Horňáková, Michaela ; Reiniš, Milan (advisor) ; Drbal, Karel (referee)
Cancer treatment includes the use of chemotherapeutic agents, which have various effects on tumour cells, such as direct toxicity to cancer cells, immunogenic cell death induction and changes in cancer cells phenotype. Throughout the last decade many researchers have been focusing on the induction of genotoxic stress and cellular senescence, which chemotherapy can trigger. Even though induction of senescence in cancer cells represents an important mechanism for tumour suppression, there has been increasing evidence that shifting cancer cells into a senescent state by chemotherapy is not always beneficial. Senescent cells are associated with a specific secretory phenotype, which allows such cells to alter their microenvironment, modulate anti-tumour immunity, induce tumour suppression and even promote cancer development. Therefore, senescent cells elimination by innate or specific immunity, which can be boosted by immunotherapy, can be an important barrier preventing tumour growth. Powered by TCPDF (www.tcpdf.org)
|
guest ::
