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Active security equipment in computer networks
Škrdla, Vojtěch ; Martinásek, Zdeněk (referee) ; Malina, Lukáš (advisor)
The main object of this bachelor's thesis is to describe problematics of active security devices. The thesis is mainly focused on firewalls. Thesis is divided into several topics, in which we describe types of security devices, division of firewalls, description of their advantages and disadvantages, types of VPN. The practical part describes the security design of mid-sized computer network, description of software that was used to test this network and the last part focuses on designing laboratory tasks with firewalls Cisco ASA5520 and Checkpoint Gaia R77.
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Processing of images taken from UAV
Roubal, Pavel ; Plánka, Ladislav (referee) ; Hanzl, Vlastimil (advisor)
The aim of the thesis was to process the images taken by UAS technology and especially to test the accuracy of this method on the checkpoints of various types. For this testing were used checkpoints that were measured by geodetic method. Subsequently these points were compared with points measured photogrammetrically.
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The role of truncated PPM1D/Wip1 phosphatase in cancer
Martiníková, Andra-Stefania ; Macůrek, Libor (advisor) ; Souček, Pavel (referee) ; Mistrík, Martin (referee)
When encountering damage, the cells activate the DNA Damage Response (DDR) pathway and stop the cell cycle until the DNA is repaired. PPM1D/WIP1 phosphatase resumes the cell cycle after the damage has been repaired, by directly dephosphorylating DNA damage markers. The DDR pathway prevents genome instability or cancer development. Mutations in the Ppm1d gene encoding PPM1D result in an overstable and truncated protein observed both in cancer patients and in cancer cell lines. In this thesis, we used an "in-house" transgenic mouse model in which mutations in the exon 6 of the Ppm1d gene resulted in a truncated PPM1D protein. First, we observed high PPM1D levels and impaired DDR to gamma ionizing radiation (IR) in the mouse thymi having truncated PPM1D (Ppm1dT/+ ). We then bred the Ppm1dT/+ mice with the Trp53+/- heterozygote knock-out mice which are prone to thymic lymphoma. The Ppm1dT/+ Trp53+/- double-mutants had a higher frequency of developing IR-induced T-cell lymphomas, compared to the single Trp53+/- mutants. Moreover, truncated PPM1D leads to a defective cell cycle checkpoint activation in human non-transformed RPE cells (RPE1), which then proliferate despite the presence of DNA damage. RPE1 cells also display increased proliferation after replication stress. RPE1 or U2OS cells with...
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Molecular mechanisms of checkpoint signalling and termination
Benada, Jan ; Macůrek, Libor (advisor) ; Brábek, Jan (referee) ; Truksa, Jaroslav (referee)
Cells employ an extensive signalling network to protect their genome integrity, termed DNA damage response (DDR). The DDR can trigger cell cycle checkpoints which prevent cell cycle progression and allow repair of DNA damage. The failures in these safeguarding mechanism are represented by serious human malignancies, most predominantly by cancer development. This work aims to contribute to the understanding of how do the cells negatively regulate DDR and cell cycle checkpoint signalling. We focused mainly on Wip1 (PPM1D) phosphatase, which is a major negative regulator of DDR and is indispensable for checkpoint recovery. Firstly, we have shown that Wip1 is degraded during mitosis in APC-Cdc20 dependent manner. Moreover, Wip1 is phosphorylated at multiple residues during mitosis, resulting in inhibition of its enzymatic activity. We suggest that the abrogation of Wip1 activity enables cells to react adequately even to low levels of DNA damage encountered during unperturbed mitosis. In the following publication, we have investigated why the mitotic cells trigger only early events of DDR and do not proceed to the recruitment of DNA repair factors such as 53BP1. We showed that 53BP1 is phosphorylated within its ubiquitination-dependent recruitment domain by CDK1 and Plk1. These phosphorylations prevents...
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Molecular mechanisms of checkpoint signalling and termination
Benada, Jan ; Macůrek, Libor (advisor) ; Brábek, Jan (referee) ; Truksa, Jaroslav (referee)
Cells employ an extensive signalling network to protect their genome integrity, termed DNA damage response (DDR). The DDR can trigger cell cycle checkpoints which prevent cell cycle progression and allow repair of DNA damage. The failures in these safeguarding mechanism are represented by serious human malignancies, most predominantly by cancer development. This work aims to contribute to the understanding of how do the cells negatively regulate DDR and cell cycle checkpoint signalling. We focused mainly on Wip1 (PPM1D) phosphatase, which is a major negative regulator of DDR and is indispensable for checkpoint recovery. Firstly, we have shown that Wip1 is degraded during mitosis in APC-Cdc20 dependent manner. Moreover, Wip1 is phosphorylated at multiple residues during mitosis, resulting in inhibition of its enzymatic activity. We suggest that the abrogation of Wip1 activity enables cells to react adequately even to low levels of DNA damage encountered during unperturbed mitosis. In the following publication, we have investigated why the mitotic cells trigger only early events of DDR and do not proceed to the recruitment of DNA repair factors such as 53BP1. We showed that 53BP1 is phosphorylated within its ubiquitination-dependent recruitment domain by CDK1 and Plk1. These phosphorylations prevents...
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Active security equipment in computer networks
Škrdla, Vojtěch ; Martinásek, Zdeněk (referee) ; Malina, Lukáš (advisor)
The main object of this bachelor's thesis is to describe problematics of active security devices. The thesis is mainly focused on firewalls. Thesis is divided into several topics, in which we describe types of security devices, division of firewalls, description of their advantages and disadvantages, types of VPN. The practical part describes the security design of mid-sized computer network, description of software that was used to test this network and the last part focuses on designing laboratory tasks with firewalls Cisco ASA5520 and Checkpoint Gaia R77.
|
|
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Processing of images taken from UAV
Roubal, Pavel ; Plánka, Ladislav (referee) ; Hanzl, Vlastimil (advisor)
The aim of the thesis was to process the images taken by UAS technology and especially to test the accuracy of this method on the checkpoints of various types. For this testing were used checkpoints that were measured by geodetic method. Subsequently these points were compared with points measured photogrammetrically.
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