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Hair as a bioindicator of contamination of risk metals
Zmijová, Marie ; Řezáčová, Veronika (referee) ; Zlámalová Gargošová, Helena (advisor)
The use of hair as a bioindicator of environmental contamination by risk elements is becoming more and more common. This bachelor thesis deals with the use of this matrix for analysis using the voltammetric method. It focuses on the optimization of the method for determining the concentration of cadmium, copper, lead and zinc in real hair samples and monitors the effects on the incorporation of these elements into the hair fiber. The presence of zinc and copper was confirmed in all samples analyzed. Cadmium could only be quantified in one sample. The effect of gender and age on the concentration of elements has not been demonstrated. Concentration of lead has been linked to smoking, with higher concentrations found in smoker’s hair than in non-smoker’s.
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Determination of palladium by ET-AAS after previous preconcentration by solid phase extraction
Hajdová, Jana ; Čelechovská, Olga (referee) ; Komendová, Renata (advisor)
The theoretical part of this master’s thesis focuses on the characteristic of Palladium, his uses in the automotive convertors, the occurrence in the environment, bioaccumulation and human health risks. Next there are described the basics of biomonitoring and summarize suitable biomonitoring method for capturing airborne dust. There are also evaluated appropriate methods for preconcentration and determination of Palladium. The aim of experimental part of this thesis is description and evaluation optimalization preconcentration method SPE by using sorbents silicagel C18 and QuadraSil TA. Optimization of method conditions of electrothermal atomic absorption spectrometry (ET – AAS). The determination of Palladium using optimal conditions follows in real samples in the form of exposure lichen (Hypogymnia physodes) by automobile traffic in Brno.
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Bioindicators - their role in the evaluation of environment
Winklerová, Lucie ; Doležalová Weissmannová, Helena (referee) ; Zlámalová Gargošová, Helena (advisor)
This thesis deals with the use of plant and animal bioindicators in an evaluation of the state of the environment. Bioindicators are divided in this work according to ecosystem for which they are used into bio indicators of terrestrial and of water ecosystem. Furthermore, they are divided according to groups of analytes, which are indicated by means of them. In the work the attention is paid to selected elements of risk, polycyclic aromatic hydrocarbons and polychlorinated biphenyls. The presence of these xenobiotics in the environment is particularly influenced by human activities. First of all individual industrial and automobile transports are permanent sources of contamination of the ecosystem with these xenobiotics. Considerable influence on the ecosystem pollution with organic and inorganic contaminants had also an injudicious application of certain fertilizers in agricultural production. The importance of bioindication and bioindicators is big. They help us not only assess the presence of xenobiotic in the environment, the development of its occurrence and sources of pollution, but they are also necessary in the evaluation of its biological effect, and responses and mechanisms of the organism which are induced by it. Biomonitoring is now an essential part of environmental protection by reason of monitoring the presence and the transport of contaminants.
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Vliv intenzity hospodaření při obnově dubových porostů v oblasti soutoku Dyje a Moravy na diverzitu pavouků (Araneae)
Rychlá Hulejová, Petra
In this thesis, the intensity of management (presence of mechanical soil preparation) of the post-harvest stand and its effect on spider communities was investigated. There were chosen ten locations for the research, which were divided into 5 intensively managed and 5 extensively managed plots. A ground trapping transect was placed in each plot. Sampling were made on the following dates: 18 May 2021, 17 June 2021, 19 July 2021, 19 August 2021, 01 October 2021. A total of 7001 individuals belonging to 16 families and 85 species were captured, of which 12 species were on the Red List of Protected Species in the Czech Republic. Pardosa lugubris was the most abundant species and Trochosa spinipalpis from the Red List species. The most abundant family found during the survey was the representative of the family Linyphiidae. Primarily individuals with a ground hunting strategy were captured in the ground trapping survey. However, a number of net species were also present. The number of species was significantly higher in areas extensively managed without mechanical soil preparation. The conservation value and abundance of rare and endangered species was also significantly higher in plots under extensive management. It was also found that the effect of management did not affect the overall abundance of spiders. Significant factors influencing spider species composition included vegetation cover and the presence of small wood residues covering the soil surface. According to the results, it can be concluded that extensive management has a positive effect on spiders. Therefore, we recommend this type of management as a positive method that could be further used in the restoration of oak forests in the Soutok Forest Reserve, or in other areas with valuable floodplain forests.
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Abnormal shapes of diatoms induced by elevated concentrations of chemical substances.
Budd, Dagmar Marguerite ; Kulichová, Jana (advisor) ; Woodard, Kateřina (referee)
Traditional biomonitoring methods are compared in this thesis with a modern approach which uses fluctuation asymmetry to measure developmental instability and refines biomonitoring methods in order to emphasis the impact of toxigenic substances in freshwater environments. The next section provides information about different types of pollution, with the focus primarily on heavy metal contamination and the impact from increased concentration of these metals on diatom frustules, green algae and other protist organisms. The most common cause of teratological changes comes from heavy metals and the impact of them along with toxicity are analyzed here. What changes is the teratology at the intracellular level and the protist organism morphology, where the cause of the changes can be traced to these heavy metals. The thesis also compares the impact of the heavy metals among different protist organism groups and between each of them. It contains suggested phycoremediation, which in the future could be an ecological solution for the cleanup of rivers and streams. Keywords: fluctuation asymmetry, developmental instability, geometric morphometrics, teratologies, diatoms, morphological asymmetries, biomonitoring
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Dynamics and structure of diatom communities in glacier and/or groundwater-fed streams.
Prüherová, Alžběta ; Kulichová, Jana (advisor) ; Žárský, Jakub (referee)
Alpine streams and their algal communities have been increasingly challenged in recent years by the effects of climate change, particularly associated with glacier retreat and their melting. Glaciers in particular are an important hydrological source for alpine ecosystems, which are threatened by rising temperatures, that are more influential in these areas than at lower altitudes. Due to the ongoing retreat of glaciers, glacier meltwater is increasingly being added to tributary streams. This glacier water has significantly different characteristics and may thus be a significant contributor to changes in the diatom biodiversity of alpine aquatic ecosystems. The manipulated experiment of this thesis took place from July to September 2020 in the Swiss Alps. Using permanent diatom slides created out of samples taken from the experimental channels, changes occurring when glacier meltwater was added to ground water-fed tributary streams and changes occurring when ground water was added to glacier-fed streams were analysed. Changes in diatom abundances were compared with environmental factors such as temperature, pH, turbidity or conductivity. Glacial streams were characterised by significantly lower temperature, higher values of conductivity and turbidity, higher oxygen saturation and also higher pH...
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Use of mosses for bioindication of atmospheric deposition
Ullmannová, Adéla ; Hůnová, Iva (advisor) ; Vindušková, Olga (referee)
Atmospheric deposition of pollutants is a significant environmental problem that can have a serious impact on ecosystems and human health. Therefore, monitoring this process is essential to assess its impacts and develop effective mitigation strategies. This bachelor's thesis is a literature review that deals with the use of mosses as bioindicators of atmospheric deposition. Mosses are sensitive to environmental changes and can accumulate pollutants from the atmospheric deposition, making them valuable and widely utilised bioindicators of the atmospheric deposition loads. The thesis summarizes the different types of pollutants that can be deposited in the atmosphere and the methods used to collect and analyze moss samples. It also discusses factors that influence the response of mosses to atmospheric deposition, such as species differences, geographical location and environmental conditions. It examines the advantages and limitations of their use as bioindicators. Further research is needed to improve the understanding of the relationship between mosses and atmospheric deposition and to design a unified protocol that standardizes methods and increases their effectiveness for international monitoring of environmental pollution. The work highlights the importance of monitoring atmospheric deposition...
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Diatoms as bioindicators for tracking freshwater salinization.
Severa, František ; Kopalová, Kateřina (advisor) ; Dobiáš, Jakub (referee)
The focus of this thesis is on the relationship of diatom (Bacilariophyta) communities to conductivity, and the utilization of this relationship for the monitoring of freshwater salinization. Salinization of freshwaters as a consequence of anthropogenic influences is emerging as a threat to many freshwater habitats due to increasing anthropogenic sources of salts (like use of de-icing salts on roads), wastewaters, and fertilizers. The problem is intensifying because salts are further concentrated through increased evaporation as a consequence to climatic changes. Salinization of ecosystems has a direct impact on the physiological processes of present organisms, and because the ability to adapt to this stress differs between organisms, results in the reorganization of communities. In this way, salinization can indirectly threaten ecosystem services that benefit mankind. Furthermore, salinization is a direct threat in terms of its influence on drinking water and crop irrigation. These forces also act on diatoms, which have long been used as bioindicators, and exhibit strong community links with conductivity. Like for other organisms, this tight relationship is caused by differences in adaptation mechanisms between species, which includes osmolytes, extracellular polymeric substances and adaptation of...
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