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Analysis of active substances in instant beverages for child´s nutrition
Jašková, Martina ; Kočí, Radka (referee) ; Márová, Ivana (advisor)
The aim of presented diploma thesis was to characterize baby beverages for early infant feeding. Theoretical part deals with baby foods and beverages including breast milk. Practical part is focused on analysis of active substances in 21 commercially available fruit and herbal instant granular baby beverages and in 3 packaged tea bags for infant and toddler age. To evaluation of antioxidant properties some group parameters were used - total polyphenols, total flavonoids, total antioxidant activity and anthocyanins. All of them were analysed by using spectrophotometrical methods. For identification and quantification of individual phenolic substances was used RP-HPLC/UV-VIS and HPLC/PDA/LC-MS, identification of individual mono- and disaccharides was performed by HPLC/RI. L-ascorbic acid supplemented to some children's drinks was analysed by HPLC-NH2/UV. Protein analysis was performed by Experion microfluidic electrophoresis system (BioRad) and protein concentration was verified by colorimetric method. In general, the highest content of phenolic compounds was shown in tea bags when compared with instant granular beverages that contain mostly added simple carbohydrates. Using contact microbial tests proved immediately after opening and also during manipulation with tea boxes was verified that analysed baby teas are in agreement with strict hygiene requirements for baby beverages.
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Analysis of active substances in instant beverages for child´s nutrition
Jašková, Martina ; Kočí, Radka (referee) ; Márová, Ivana (advisor)
The aim of presented diploma thesis was to characterize baby beverages for early infant feeding. Theoretical part deals with baby foods and beverages including breast milk. Practical part is focused on analysis of active substances in 21 commercially available fruit and herbal instant granular baby beverages and in 3 packaged tea bags for infant and toddler age. To evaluation of antioxidant properties some group parameters were used - total polyphenols, total flavonoids, total antioxidant activity and anthocyanins. All of them were analysed by using spectrophotometrical methods. For identification and quantification of individual phenolic substances was used RP-HPLC/UV-VIS and HPLC/PDA/LC-MS, identification of individual mono- and disaccharides was performed by HPLC/RI. L-ascorbic acid supplemented to some children's drinks was analysed by HPLC-NH2/UV. Protein analysis was performed by Experion microfluidic electrophoresis system (BioRad) and protein concentration was verified by colorimetric method. In general, the highest content of phenolic compounds was shown in tea bags when compared with instant granular beverages that contain mostly added simple carbohydrates. Using contact microbial tests proved immediately after opening and also during manipulation with tea boxes was verified that analysed baby teas are in agreement with strict hygiene requirements for baby beverages.
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Segmentation of Infant Milks Market
Čevela, Josef ; Koudelka, Jan (advisor) ; Sobol, Robert (referee)
The principal goal of the master thesis is to explore the similarities and possible differences among customers of infant milks. Based on those findings, the customers are clustered into various segments, which are extremely homogenous within each one, but significantly heterogeneous outside each of them. The second goal of the thesis is to suggest the best marketing solutions for each revealed segment. The work is divided into two parts, theoretical and practical. The theoretical part describes the process of market segmentation. The characteristics of the infant milk market and the description of infant milk types are described in the practical part. The customers' segmentation is designed according to primary and secondary research. Secondary data is based on data from project MML-TGI and content analysis. The data from my own primary research are processed by IBM SPSS statistics version 20. Among the main finding of the thesis was defining 5 segments of the infant milk customers. The work characterizes all of them and special term is chosen for each of the segment. Finally, the relevant marketing solutions are suggested for each of the segment.
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