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Magnetic nanoparticles with antibacterial properties: Synthesis, characterization and biological applications
Shatan, Anastasiia-Bohdana ; Horák, Daniel (advisor) ; Kaman, Ondřej (referee) ; Matějíček, Pavel (referee)
In response to the escalating global threat of antibiotic resistance, innovative strategies are imperative. This thesis focuses on surface-engineered magnetic nanoparticles (MNPs) with potent antibacterial properties, aiming to combat antibiotic resistance effectively. Specifically, uniform 16-nm Fe3O4 nanoparticles were synthesized via oleic acid-stabilized thermal decomposition of Fe(III) oleate in a high-boiling organic solvent. Optionally, 8-nm γ-Fe2O3 particles were obtained by coprecipitation of Fe2+ and Fe3+ salts in a basic medium. For the application of antibacterial MNPs in biological media, water-dispersible nanoparticles were required. Hence, original magnetic particles containing hydrophobic oleic acid (OA) coating were modified with silica using a water-in-oil reverse microemulsion. Subsequent modification with (3-mercaptopropyl)trimethoxysilane and decoration with silver nanoclusters yielded Fe3O4@SiO2-Ag nanoparticles. Additionally, neat Fe3O4 particles were coated with Sipomer PAM-200 containing both phosphate and methacrylic groups, facilitating attachment to the iron oxide and enabling (co)polymerization with 2-(dimethylamino)ethyl methacrylate and/or 2- tert-butylaminoethyl methacrylate. Furthermore, γ-Fe2O3 nanoparticles were rendered antimicrobial through modification with...
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Plasmachemical preparation of polymer layers based on 2-ethyl-2-oxazoline and their diagnostics
Podzemná, Erika ; Menčík, Přemysl (referee) ; Mazánková, Věra (advisor)
The bachelor’s thesis deals with deposition of thin polymer layers on 2-ethyl-2-oxazolin basis in a dielectric barrier discharge in a nitrogen atmosphere. Polyoxazolines are characterized by good antibacterial effects in general, which make them a suitable material for medical applications. After preparing of the layers. Their properties were studied using three methods. The first were antibacterial tests, which unfortunately did not confirm the above written properties. Furthermore, the free surface energy of the layers, from which it could be inferred that the surfaces were hydrophilic, was determined by measuring contact angles. The scanning electron microscopy showed the surface of the layers as well as the elemental composition thanks to the connected X-ray detector.
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Plasma chemical deposition of thin polymer layers based on 2-ethyl-2-oxazoline and their diagnostics
Podzemná, Erika ; Horák, Jakub (referee) ; Mazánková, Věra (advisor)
Medical devices are often subject to the colonisation of bacteria, making it a prerequisite for infection in the patient's body. To prevent biofilm, the medical device can be covered with a thin layer of polymeric substance that has the ability to prevent biological fouling. In this work, was selected 2-ethyl-2-oxazoline as a precursor for the formation of thin polymer layers. In general, the oxazoline group is known for its antibacterial and biocompatible properties, which is the reason for choosing this substance. The main objective of this thesis was the deposition of thin polymer films, which are based on 2-ethyl-2-oxazoline, in surface dielectric barrier discharge under atmospheric pressure. The principle of thin layer formation on a teflon substrate is plasma polymerization. The preparation of the layers was followed by their characterization by several methods. Their physical and chemical properties were studied using SEM, FTIR, and by measuring the contact angles of the surface of the layers. Furthermore, the processes that take place in plasma, when there is an interaction of electrons and ions with the monomer molecule, were investigated. Ion mobility spectrometry and the crossed-beam ionization method were used for this measurement. The layers were also tested for their antibacterial and cytocompatible properties.
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Deposition and diagnostics of plasmachemical prepared thin polymer layers based on 2-methyl-2-oxazoline
Podzemná, Daniela ; Slavíček, Pavel (referee) ; Mazánková, Věra (advisor)
The deposition of thin polymer layers, which are antibacterial and cytocompatible at the same time, is one of the solutions to prevent infection when using biomaterials in biomedical applications. The formation of layers based on plasma polymerization in the surface dielectric barrier discharge, which was used in the presented work, seems promising in terms of time and financial simplicity. When we use the plasma polymerization method, it should be possible to create a layer containing such functional groups that will have the ability to repel unwanted bacterial contamination. The analyte used in this work to form such layers is the monomer 2-methyl-2-oxazoline. Plasma polymerization of this substance produces polyoxazolines, which are known for their antibacterial and cytocompatible properties. Part of the work also deals with polytetrafluoroethylene, which in our case is substrate for deposition. The subject of the experimental work was the subsequent characterization of the layers using biological diagnostic methods, which were antibacterial tests and cytocompatibility tests in vitro. The physico-chemical properties of the layers were investigated using SEM, FT-IR and the surface free energy determination method. The experimental part also included the study of interactions of electrons and ions with molecules taking place in the plasma.
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Plasmachemical preparation of polymer layers based on 2-ethyl-2-oxazoline and their diagnostics
Podzemná, Erika ; Menčík, Přemysl (referee) ; Mazánková, Věra (advisor)
The bachelor’s thesis deals with deposition of thin polymer layers on 2-ethyl-2-oxazolin basis in a dielectric barrier discharge in a nitrogen atmosphere. Polyoxazolines are characterized by good antibacterial effects in general, which make them a suitable material for medical applications. After preparing of the layers. Their properties were studied using three methods. The first were antibacterial tests, which unfortunately did not confirm the above written properties. Furthermore, the free surface energy of the layers, from which it could be inferred that the surfaces were hydrophilic, was determined by measuring contact angles. The scanning electron microscopy showed the surface of the layers as well as the elemental composition thanks to the connected X-ray detector.
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Povrchové úpravy nábytku vhodné do veřejných prostor zaměřené zejména na antibakteriální vlastnosti
Kolesnik, Iuliia
The properties of the two surface finishes were studied in this work: the waterborne epoxy lacquer EPOX AQUA V_1301 and solvent-borne alkyd urethane lacquer Jachtlak S-1006 with the regards to their potential applications for the finishing of the wood surfaces of furniture designed for the utilization in public places. It has been found that the dry surfaces formed by these furnishes have physico-mechanical properties that are consistent with the standards, which establish possibilities of their application. Very good antibacterial properties of the formed films without the addition of any auxiliary antimicrobial agents to the Escherichia coli and Staphylococcus aureus bacterial strains were also determined. The entire tests were carefully described, and their results were discussed in the further text.
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Antibakteriální vlastnosti povrchových úprav pracovních kuchyňských desek
Endlicherová, Veronika
The diploma thesis deals with antibacterial properties of kitchen worktops surface finishes. The thesis compares the antibacterial, physical-mechanical, utility and appearance properties of various surface treatments on solid wood of oak, acacia and pear wood. The research was carried out on alkydurethane and water-borne varnish. Escherichia coli and Staphylococcus aureus were used for the assesment of antibacterial activity. The thesis describes the risks associated with microorganisms and kitchen environment. Furthermore, the requirements for surface finishes of kitchen worktops were analyzed.
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