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Stimulation of early child development according to "Estimulacion temprana"
Maryšková, Tereza ; Sotáková, Hana (advisor) ; Presslerová, Pavla (referee)
In my work I focused on the preventive group program Estimulación temprana, which is intended for children from 0 to 3 years old and their parents. Six case studies are presented to help analyze this program. Research has revealed that the program is working on children through coarse and fine motor, cognitive, speech and social development. The case reports indicate that the observed individuals did not deteriorate, on the contrary, there was a shift in the Brunet-Lezin scale. Activities take into account the developmental level of groups in which children are disaggregated by age, but the individual level of specific individuals reflects only to a small extent. The study also shows that Estimulación temprana leads parents to better orientation in education, targeted child care and out-of-school lessons and also helps them with effective ways of stimulating. From the subjective point of view of parents, the contribution of Estimulación Temprana lies above all in the possibility of sharing experiences, deregistering, establishing friendly relationships, and changing educational leadership. The results of this work provide a qualitative analysis in our not known program Estimulación temprana, whose specificity lies in the orientation to the whole of child development, thus being distinguished among...
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Rearing of weatherfish (Misgurnus fossilis) fry in artificial condition
FRANTA, Pavel
The aim of the M.Sc. thesis was to verify possibility of weaning weatherfish larvae (Misgurnus fossilis) from the live food to the dry starter mixture using the method of co-feeding. The experiment included 3 control groups (starved control no feeding; negative control fed exclusively with dry starter mixture; positive control fed exclusively with live food) and 10 experimental groups with 5 different dates of termination of co-feeding period (at the age 13, 18, 23, 28 and 33 days post-hatching dPH) and different duration of co-feeding period (1 and 7 days). The experiment lasted from the 6 dPH, when mixed feeding was iniciated, until the age 48 dPH at the temperature of 17,8 +- 0,7 °C. The effects of termination and duration of co-feeding period upon continuous survival, morphometric and gravimetric characteristics, ratio of macrobiogenic elements and gross caloric value in somatic tissues of larvae/juveniles were evaluated. Continuous survival, morphometric and gravimetric characteristics and total content of carbon and nitrogen in somatic tissues of larvae/juveniles were increasing with extending dates of termination of co-feeding period. The rearing of weatherfish larvae was accompanied by the marked decline of survival in the period 13 20 dPH, regardless of selected diet regimes in individual groups. Within all fed groups, the worst results of continuous survival and growth were recorded in the negative control as well as groups with date of co-feeding period termination at age 13 dPH. All individuals in these groups died at age 41 dPH. At the end of the experiment, the highest values of continuous survival were achieved in the positive control as well as groups with dates of co-feeding period termination at age 23, 28 and 33 dPH, respectively. There was no significant statistical difference among these groups. Very good results in terms of morphometric and gravimetric characteristics were achieved in the groups with dates of co-feeding period termination at age 28 and 33 dPH, respectively. However, only in group with date of co-feeding period termination at age 33 dPH were achieved comparable values of morphometric characteristics with the positive control. The exception formed only values of the body height as well as dry and wet weights which were significantly higher in the positive control compared to other fed groups. The duration of the co-feeding period (either 1 or 7 days) did not affect any of the monitored characteristics. The results of the present thesis also show that the weatherfish fry best prospers on live food and, on the contrary, fry can not be reared using dry starter mixture as a sole food source. To sum up, among all tested groups that use co-feeding, only weaning of larvae with the date of co-feeding termination at age 33 dPH could be advised. In such condition the continuous survival rate as well as lenght growth reach comparable values with group exclusively fed by live food.
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Vliv triazinových pesticidů na ryby
STARÁ, Alžběta
Fish and crayfish are widely used as biological monitors of environmental levels of anthropogenic pollutants. The present thesis is a contribution to the assessment of the toxicity and effects of long-term effect of triazines on the different developmental stages of common carp (Cyprinus carpio L.) and adult red swamp crayfish (Procambarus clarkii). The carp was selected as a model fish due to its economic importance, e.g. carp farming contributes about 90% to total fish production in the Czech Republic. Crayfish are easily identified species representing given locality, they are widespread, and they provide a sufficient amount of tissue for individual biochemical and chemical analyses. The results of these studies provide further data on chronic exposure to triazines for consideration in risk assessment. We selected three active substances of triazine herbicides which are the most frequently detected in surface waters such as prometryne, simazine and terbutryn. The findings contribute to knowledge of the toxic potential of triazine herbicides to carp and crayfish at environmentally relevant concentrations in Czech rivers. There is a scarcity of information regarding the toxicity of triazines on freshwater organisms. During the tests we monitored several parameters: behaviour, mortality, biometric, haematological, biochemical blood, histopathology, oxidative stress and antioxidants. The data obtained from all tests performed during my thesis are very valuable for assessment and evaluation of long-term effects xenobiotics on aquatic organisms, especially fish and crayfish. In the future, I would extend the focus of my research for study of possible synergic or amplifying effect of mixtures triazines with other xenobiotics which are often found in the aquatic environment. This approach is recently accented as the aquatic environment is polluted by mixtures of different compounds. Therefore, more research is needed in order to clarify the more detailed effects of xenobiotics on non-target aquatic organisms.
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The effect of water temperature on early ontogeny in African catfish (Clarias gariepinus)during post - embryonic development
FRANTA, Pavel
This theses is made up from two methodologically identical experiments, which involved observing the starting time of key sections of early ontogeny (H95, S95, Re95) at a temperature of 24°C. The experiments used 29 groups of eleuterembryoes/larvae which were fed for 30 days, but timing of initial feeding of exogenous food was different between the groups. The first group began initial feeding at the age 72 hPF and every other group was fed for the first time 6 hours later than the previous group (the last group was fed at the first time at the age 240 hPF). The group, which wasn´t fed exogenous food during the experiment, served as control. The effect of timing of start feeding on morphometric and gravimetric characteristics, size distribution, survival and rate of cannibalism in eleuterembryoes/larvae was examined. A temperature of 24 °C was determined the hatching time of eleuterembryoes after 36, resp. 33 hours (1st, respectively 2nd experiment) from fertilization. The start of intake of exogenous food (S95) was determined at age 123 hPF, yolk sac absorption at age 150 hPF during the 1st experiment. At the 2nd experiment the start of intake exogenous food was determined at age 115 hPF and yolk sac absorption at age 161 hPF. During the interval from H95 to S95 records showed an increased rate of growth, especially the caudal part of the body. At the age from S95 to Re95, growth of unfed larvae was stopped (first effect of starvation). Almost all monitored parametres were significantly affected (P < 0,01) by timing of start feeding except for rate of cannibalism. While the percentage representation of large sized and medium sized individuals (the potential cannibals) was decreased with increasing the time of start feeding, the rate of cannibalism wasn´t changed. However the number of small sized individuals was increased with increasing the time of start feeding. The study of the African catfish confirmed the negative impact on survival, which is caused by a delay in initial feeding. Unlike survival, with morphometric parametres there wasn´t observed a significant decrease in values, provide the larvae was first fed before reaching the age of 216 hPF. It was also found that almost 100 % of the individuals in the control (unfed) group died at age 377 hPF. However the first indications of increasing mortality was observed in age 283 hPF. Based on the above information, the PNR (point of no return) was reached approximately at the age of 270 hPF in African catfish
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