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Potential and limits of cultivation of brackish and freshwater populations of tubular morphotypes of Ulva (Ulvophyceae) in food industry or biotechnologies
Kantnerová, Veronika ; Neustupa, Jiří (advisor) ; Hejduková, Eva (referee)
The green macroalgae from genus Ulva are commonly used in biotechnology as a source of biomass for the production of biofuels or in bioremediation, but mostly for processing in food industry in Asia and other coastal regions. Within Europe, these algae belong to the so-called "approved novel food", however the species which are mainly used have the leaf-like morphology and they are usually imported, so the usage of local European resources is relatively very low. However the tubular morphotypes of the genus Ulva (originally included in the genus Enteromorpha) might probably be an equally promising source of biomass for the use in food industry and other sectors. They show high resistence to wide range of abiotic conditions and they occur not only in marine localities, but also in brackish or even freshwater ecosystems. Considering this, they seem to be a promising source for processing in Central Europe as well. In this study were isolated and cultivated two populations of the genus Ulva typical for the tubular morphotypes. One was from brackish locality in the Baltic sea (Ulva flexuosa) and one from freshwater locality in Czech Republic (Ulva pilifera). The aim was to evaluate their cultivation potential for their potential use in food industry. Their growth rates were observed during the...
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Impact of habitat type and long-term in vitro cultivation on photosynthetic characteristics of snow algae and their resistance to stress induced by UV radiation
Zázvorková, Michaela ; Nedbalová, Linda (advisor) ; Kvíderová, Jana (referee)
Snow algae are psychrophilic microorganisms, that inhabit snow fields in mountains and polar regions, which creates colored snow in good conditions. Most species belong to order Chlamydomonadales (Chlorophyta) with complicated life cycles, containing flagellates and immobile stages (cysts). Extreme environment of snow is characteristed by low temperature, problems with availability of water and lack of nutrition. Depending on location and phase of life cycle, snow algae have to deal with excess or lack of solar radiation, the important component of which is also dangerous UV radiation. The light conditions differ substantially from open location above the forest level or polar regions to forest habitats. In the first part of this work I compared reactions of photosynthetic apparatus of snow algae strains from forest and forestless habitats to different intensity of radiation, then I dealt with assessing any changes related to long-term cultivation in laboratory. Based on measurement of rapid light curves on PAM fluorometer I have determined some characteristics of photosynthetic apparatus of individual strains (parameters α and Ik), which indicate adaptation to low or high light intensity. For some strains, it was possible to compare the results obtained at a three-year interval. In the second part...
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