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Molecular typisation of lactic acid bacteria
Jelínek, Zdeněk ; Ing. Špano Miroslav,PhD. (referee) ; Rittich, Bohuslav (advisor)
Lactic acid bacteria play important role both in food industry and health care. They are part of many fermented dairy products and gastrointestinal microflora. There is a whole variety of methods for bacteria identification, but we will focus on Rep-PCR and RAPD only. The aim of this work will be to discuss the impact of different correlation coefficients and cluster analysis algorithms on the results of taxonomic analysis.
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Detection of chimeras in amplicon sequencing
Heřmánková, Kristýna ; Jurečková, Kateřina (referee) ; Sedlář, Karel (advisor)
Chimeric sequences are the most common artifacts that can occur in sequencing data after the sample amplification using the polymerase chain reaction. The presence of these artifacts can negatively affect results of the analysis. Therefore, the detection and subsequent filtration of chimeric sequences is an important step in the computational processing of sequencing data. This work deals with the principle of chimera formation and the possibility of reducing their occurrence. The aim of this work is to implement an algorithm for chimeras detection in R language and testing its accuracy on data provided by the Veterinary Research Institute in Brno.
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Detection of chimeras in amplicon sequencing
Heřmánková, Kristýna ; Jurečková, Kateřina (referee) ; Sedlář, Karel (advisor)
Chimeric sequences are the most common artifacts that can occur in sequencing data after the sample amplification using the polymerase chain reaction. The presence of these artifacts can negatively affect results of the analysis. Therefore, the detection and subsequent filtration of chimeric sequences is an important step in the computational processing of sequencing data. This work deals with the principle of chimera formation and the possibility of reducing their occurrence. The aim of this work is to implement an algorithm for chimeras detection in R language and testing its accuracy on data provided by the Veterinary Research Institute in Brno.
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Molecular typisation of lactic acid bacteria
Jelínek, Zdeněk ; Ing. Špano Miroslav,PhD. (referee) ; Rittich, Bohuslav (advisor)
Lactic acid bacteria play important role both in food industry and health care. They are part of many fermented dairy products and gastrointestinal microflora. There is a whole variety of methods for bacteria identification, but we will focus on Rep-PCR and RAPD only. The aim of this work will be to discuss the impact of different correlation coefficients and cluster analysis algorithms on the results of taxonomic analysis.
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