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National Repository of Grey Literature

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Larvae of flesh fly Neobellieria bullata as a source for novel antimicrobial peptides Neubauerová, Tereza ; Macková, Martina ; Macek, Tomáš ; Šanda, Miloslav ; Voburka, Zdeněk The antibiotic resistant strains of microorganisms are increasing threat for public health. Antimicrobial peptides from natural sources display a wide spectrum of activity. In our laboratory, several peptide fractions with antimicrobial acivity against gram-positive and gramnegative bacteria and fungi were isolated from larvae of flesh fly Neobellieria bullata. The presence of peptides was proved by tricine electrophoresis, molecular weights and sequences determination by MS and Edman sequencing, respectively. In comparison with database sequences we obtained structures present mainly in insect and amphibian antimicrobial peptides, but total matches were not higher than in 36%. Upon the obtained sequence motifs we designed structures of several synthetic peptides. These were synthetised on the Rink amide MBHA resin. Synthesis was performed according Fmoc/PyBOP-DIEA protocol. Antimicrobial activity of these peptides was determined. Detailed record

Distribuce a degradace oostatických peptidů v masařce Neobellieria bullata Hlaváček, Jan ; Bennettová, Blanka ; Slaninová, Jiřina ; Černý, B. ; Vlasáková, Věra ; Holík, Josef ; Ježek, Rudolf ; Tykva, Richard Distribution and degradation of oostatic peptides in the selected organs of flesh fly Neobellieria bullata was investigated using tritium labeled molecules. The degradation of peptides proceeded fast at both the amino and carboxy termini and resulted in formation of transient tetra and tripeptide sequences. Detailed record

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