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Design of the Multicore Processor in VHDL
Novotný, Jaroslav ; Straka, Martin (referee) ; Kaštil, Jan (advisor)
The objective of the thesis is to design and implement in the VHDL language a simple multiprocessor supporting parallel computing. Furthemore, the author has designed and realized universal transparent generic interconnection layer with the objective to connect any given number of processor cores to shared address space using arbitrated bus. Parametrized cache has been allocated to each core in the layer. MSI protocol was used to deal with the issue of memory coherence of the implemented system. Direct and indirect synchornisation support is available to the user. In order to verify the functionality of the system, simple processor core has been designed and implemented, and its copies were connected to the interconnection layer. Various testing programmes have been used to verify the functionality of the system, which also confirmed that the acceleration of computing has been achieved successfully. Virtex6 chip has been used to test the whole system.
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Emulation of Memory Subsystems in Multiprocessor Environment
Rajčok, Andrej ; Husár, Adam (referee) ; Přikryl, Zdeněk (advisor)
This study cover problem of design of emulation platform for multiprocesors systems. This study discuss problem of multiprocessor communication, it discuss different kind of communication systems for multiprocesor communication and protocols for data coherency. One of the solutions is picked, and the design is made and implemented into integrated design enviroment. In the end, study analyze created emulation system with focus on performance and memory load.
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Maintenance of Small Transport Aircraft with Application MSG-3 Methodology
Trefilová, Helena ; Třetina, Karel (referee) ; Finda, Jindřich (advisor)
Master’s thesis deals with problems of maintenance of small transport aircraft with application MSG-3 methodology and maintenance plan development. It is aimed at systems and powerplant maintenance. This method is applied on L-410UVP-E20 aircraft. Other parts of this work are assessment of recent situation in maintenance of airplanes, used approaches to maintenance, methods and documentation for maintenance. Last part of this work is practical example of MSG-3 process on assign item and its interpretation.
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Application of mass spectrometry methods for identification and localization of important molecules from parasitic helminths
Siegel, Tomáš ; Kašný, Martin (advisor) ; Harant, Karel (referee)
The thesis summarises the fundamental technological solutions, processes and principles of mass spectrometry with special focus on their use in parasitology. In the first part, the particular phases of processing of the biological sample by using mass spectrometry are defined - from ionisation to the evaluation of results. In the text, the various methodological approaches are presented mainly on the published examples concerning the identification of molecules from parasitic helminths, especially proteins of fasciolid flukes (e.g. Fasciola hepatica). The second part of the thesis comprises the methods of identification and localization of molecules by using mass spectrometry imaging and the use of this combined technique in experimental biology, parasitology, is discussed. There is also proposed the procedure including the combination of laser microdissection and mass spectrometry techniques which could be applied for identification and localisation of important proteins of the fluke Fascioloides magna.
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Utilization of DESI/DAPPI-MS and MALDI-MS for studying biological objects
Kaftan, Filip
(EN) Presented PhD thesis was aimed at the utilization of desorption ionization mass spectrometric techniques to study different types of biological samples. An important part of this thesis was the construction of a universal platform for ambient ionization techniques (AIT) in mass spectrometry (MS) for the experiments performed in combination with desorption electrospray ionization (DESI) and desorption atmospheric pressure photoionization (DAPPI). Initially manual platform for AIT was during the development rebuild into a motorized platform operated by software via user interface of the mass spectrometer. Using the motorized platform it was possible to carry out a one- dimensional (1-D) and two dimensional (2-D) samples analysis with a defined step size of imaging. Platform designed for AIT in MS was tested in DESI-MS mode by model analyte Rhodamine B and by plant samples which were represented by Aconitum plicatum. The platform was also tested on biological samples of vernix caseosa using DAPPI-MS ion source. Model analyte rhodamine B was in DESI-MS technique used to study the basic desorption ionization processes occurring in the DESI during the sample analysis. In order to highlight the surface structures the colloidal solution of ferrite nanoparticles was added into the sprayed liquid. For...
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Application of mass spectrometry methods for identification and localization of important molecules from parasitic helminths
Siegel, Tomáš ; Kašný, Martin (advisor) ; Harant, Karel (referee)
The thesis summarises the fundamental technological solutions, processes and principles of mass spectrometry with special focus on their use in parasitology. In the first part, the particular phases of processing of the biological sample by using mass spectrometry are defined - from ionisation to the evaluation of results. In the text, the various methodological approaches are presented mainly on the published examples concerning the identification of molecules from parasitic helminths, especially proteins of fasciolid flukes (e.g. Fasciola hepatica). The second part of the thesis comprises the methods of identification and localization of molecules by using mass spectrometry imaging and the use of this combined technique in experimental biology, parasitology, is discussed. There is also proposed the procedure including the combination of laser microdissection and mass spectrometry techniques which could be applied for identification and localisation of important proteins of the fluke Fascioloides magna.
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Utilization of DESI/DAPPI-MS and MALDI-MS for studying biological objects
Kaftan, Filip ; Cvačka, Josef (advisor) ; Valterová, Irena (referee) ; Preisler, Jan (referee)
(EN) Presented PhD thesis was aimed at the utilization of desorption ionization mass spectrometric techniques to study different types of biological samples. An important part of this thesis was the construction of a universal platform for ambient ionization techniques (AIT) in mass spectrometry (MS) for the experiments performed in combination with desorption electrospray ionization (DESI) and desorption atmospheric pressure photoionization (DAPPI). Initially manual platform for AIT was during the development rebuild into a motorized platform operated by software via user interface of the mass spectrometer. Using the motorized platform it was possible to carry out a one- dimensional (1-D) and two dimensional (2-D) samples analysis with a defined step size of imaging. Platform designed for AIT in MS was tested in DESI-MS mode by model analyte Rhodamine B and by plant samples which were represented by Aconitum plicatum. The platform was also tested on biological samples of vernix caseosa using DAPPI-MS ion source. Model analyte rhodamine B was in DESI-MS technique used to study the basic desorption ionization processes occurring in the DESI during the sample analysis. In order to highlight the surface structures the colloidal solution of ferrite nanoparticles was added into the sprayed liquid. For...
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Utilization of DESI/DAPPI-MS and MALDI-MS for studying biological objects
Kaftan, Filip
(EN) Presented PhD thesis was aimed at the utilization of desorption ionization mass spectrometric techniques to study different types of biological samples. An important part of this thesis was the construction of a universal platform for ambient ionization techniques (AIT) in mass spectrometry (MS) for the experiments performed in combination with desorption electrospray ionization (DESI) and desorption atmospheric pressure photoionization (DAPPI). Initially manual platform for AIT was during the development rebuild into a motorized platform operated by software via user interface of the mass spectrometer. Using the motorized platform it was possible to carry out a one- dimensional (1-D) and two dimensional (2-D) samples analysis with a defined step size of imaging. Platform designed for AIT in MS was tested in DESI-MS mode by model analyte Rhodamine B and by plant samples which were represented by Aconitum plicatum. The platform was also tested on biological samples of vernix caseosa using DAPPI-MS ion source. Model analyte rhodamine B was in DESI-MS technique used to study the basic desorption ionization processes occurring in the DESI during the sample analysis. In order to highlight the surface structures the colloidal solution of ferrite nanoparticles was added into the sprayed liquid. For...
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Diagnosis of Lynch syndrome based on pathologic examination
KRAUSOVÁ, Lenka
Lynch syndrome is an autosomal dominant disease predisposing to cancer development. Up to 5 % of colorectal cancers may be associated with Lynch syndrome. Due to its familial occurrence the diagnosis is important for family screening. Currently it is based on methods of modern pathology. Theoretical part describes structure of gastrointestinal tract, definition and history of Lynch syndrome, and methods of its laboratory diagnostics. The diagnosis can be based on evaluation of tumor microscopical features (Semi PREDICT score) in tissue sections, imunohistochemical investigation of MMR (mismatch repair) proteins, or molecular genetic MSI (microsatellite instability) testing. Practical part focuses mainly on correlation of immunohistochemistry and MSI testing. Randomly selected cases of colorectal cancer were organized into 2 study groups. The first group consisted of 25 cases with intact MMR immunoexpression, the second group comprised 25 cases with at least one MMR protein being deficient. For further verification molecular genetic MSI testing, along with BRAF gene analysis and MLH1 promoter methylation status to discriminate Lynch syndrome from sporadic cases, were performed. Germline analysis of MMR genes proved the diagnosis of Lynch syndrome in 5 cases of the second group. Semi PREDICT score sensitivity for MMR-deficiency prediction (and indirectly for Lynch syndrome detection) was 84 %, specificity 48 %. Sensitivity of MSI testing was 87 %, but only 50 % in Lynch syndrome subset of cases, specificity was 100 %. The results show major role of MMR immunohistochemistry in the diagnostics of MSI-H cancers, which is the cornerstone of Lynch syndrome screening.
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