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Segmentation of soft tissues in facial part of mouse embryos from X-ray computed microtomography data
Janštová, Michaela ; Harabiš, Vratislav (referee) ; Chmelík, Jiří (advisor)
This diploma thesis deals with a segmentation of soft tissues in facial part of mouse embryos in Matlab. Segmentation of soft tissues of mouse embryos was not fully automated and every case needs a specific solution. Solving parts of this issues can provide valuable data for evolutionary biologists. Issues about staining and segmentation techniques are described. On the basis of accessible literature otsu thresholding, region growing, k-means clustering and segmentation with atlas were tested. In the end of this paper are those methods tested and evaluated on 3D microtomography data.
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The use and the effect of High Intensity Laser in treatment of rehabilitation patients
Benešová, Lucie ; Plevová, Kristýna (advisor) ; Fulín, Petr (referee)
This thesis deals with the effect of High Intensity Laser on soft tissues and postsurgical scars in the ankle joint area. The thesis is divided into two parts, theoretical and practical. The theoretical part presents a summary about ankle joint traumatology and its surgical management. Further there is also information about wound healing, scar formation, their examination, assessment scales and treatments. Last but not least there is a description about the laser mechanism, effect and treatment use. The main focus point of the practical part is to discover the effect of the High Intensity Laser used on soft tissues and postsurgical scars in the ankle joint area. A sub-objective is to evaluate the high power laser effect on pain reduction in the treated area. Methods: There were 20 probands tested in the practical part (average age 42,3, SD ± 13). The intervation group (n = 10) has applied High Intensity Laser Therapy (HILT) on postsurgical scars. The control group (n = 10) haven't HILT. The Observer Scar Assessment Scale (POSAS) was used for scar evaluation and pressure algometer was used for pain evaluation. Results: There was statistically proven that High Intensity Laser Therapy has an effect on pain reduction on level of significance α = 0,05, (p = 0,0047). According to the POSAS, results...
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Optimization of laser ablation of soft tissues
Bischofová, Hana ; Kuchyňka,, Michaela (referee) ; Pořízka, Pavel (advisor)
Laser-induced breakdown spectroscopy (LIBS) is an analytical method which is used in many fields and also for elemental analysis of soft tissues. It is necessary to optimize the soft tissue laser ablation to achieve the highest possible intensity of the wavelength spectrum. Mouse kidneys were ablated in the experimental part of this master thesis. The samples of the kidneys were prepared in two different ways, by embedding in paraffin and by preparing cryo-sections. The femto- and the nano- second lasers with different wavelengths, energies and pulse counts were used for ablation.
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Segmentation of soft tissues in facial part of mouse embryos from X-ray computed microtomography data
Janštová, Michaela ; Harabiš, Vratislav (referee) ; Chmelík, Jiří (advisor)
This diploma thesis deals with a segmentation of soft tissues in facial part of mouse embryos in Matlab. Segmentation of soft tissues of mouse embryos was not fully automated and every case needs a specific solution. Solving parts of this issues can provide valuable data for evolutionary biologists. Issues about staining and segmentation techniques are described. On the basis of accessible literature otsu thresholding, region growing, k-means clustering and segmentation with atlas were tested. In the end of this paper are those methods tested and evaluated on 3D microtomography data.
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