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Spectrophotometric determination of chlorophyll
Rábová, Petra ; Vavrčíková, Veronika (referee) ; Řezáčová, Veronika (advisor)
This bachelor thesis is focused on determination of chlorophyll in leaves by the method of extraction spectrophotometry. The theoretical part provides information about photosynthesis, stress factors or plant adaptation, photosynthetic pigments (especially chlorophyll-a) and application of spectrophotometry and other methods of chlorophyll determination. The experimental part is focused on the application of the ecotoxicological test and, above all, it is evaluation by two independent methods. Specifically, it was determination of biomass growth inhibition and newly spectrophotometrical determination of chlorophyll mass. The results correlate with each other, therefore, the determination of chlorophyll seems to be an appropriate extension of usual evaluation methods of ecological tests.
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Evaluation of the efficiency of root treatment plants via ecotoxicity tests
Konečná, Kateřina ; Procházková, Petra (referee) ; Zlámalová Gargošová, Helena (advisor)
This bachelor thesis focuses on the evaluation of the efficiency of root treatment plants using appropriately selected ecotoxicity tests. For these purposes, ecotoxicological tests in an aquatic arrangement were used, specifically with the planktonic organism Thamnocephalus platyurus and the plant Lemna minor. The evaluation was performed at a municipal root treatment plant, which treats water for 955 equivalent inhabitants. The results of tests with T. platyurus confirmed that the root treatment plant sufficiently relieves acute toxicity of water. LC50 values were determined for two samples, 213.71 ml / l for the inflow and 388.20 ml / l for the settling tank. At the outflow, these values could no longer be determined due to the low ecotoxicological effect of the water thus treated. For tests with L. minor, IC50 values were also calculated for the first two samples. For Sample No. 1, the IC50 value was calculated to be 311.25 ml / l, for Sample No. 2 to 963.35 ml / l. However, on the basis of tests of direct toxicity of waters with L. minor, it is possible to confirm even in the case of this organism, a good cleansing effect of this root treatment plant.
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Influence of microbioplastics on aquatic plants
Mácová, Sabina ; Maršálková, Eliška (referee) ; Kučerík, Jiří (advisor)
Plastic pollution is becoming a serious environmental problem, for this reason, in recent years efforts have been made to propose alternatives to conventional plastics. Biodegradable plastics appeared to be a possible alternative due to similar properties to synthetic plastics and because they are considered safe and degradable in the environment. However, under real conditions, biodegradation is a relatively time-consuming process that can lead only to biofragmentation into small particles, co-called microbioplastics. So far, very little is known about their impact on the environment. This work deals with the ecotoxicological influence of biodegradable poly(3-hydroxybutyrate) (P3HB) microparticles in two size fractions (particles
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The use of ecotoxicity tests for the use of treatment technologies
Konečná, Kateřina ; Komendová, Renata (referee) ; Zlámalová Gargošová, Helena (advisor)
This diploma thesis is focused on the assessment of the effectiveness of treatment technologies in domestic wastewater treatment plants. For these purposes, samples were taken from 3 different domestic wastewater trearment plant. Two representatives of the aquatic ecosystem, the plant Lemna minor and the freshwater crustacean Thamnocephalus platyurus, were selected for the ecotoxicity assessment. For assessment from an analytical point of view, indicators were selected according to the Government Regulation on the discharge of water into surface and underground waters No. 401/2015 Coll. and 57/2016 Coll. All the selected residential cleaning plants correspond in size to 30–500 equivalent inhabitants, while they are used by approx. 10–20 inhabitants. The results of ecotoxicological tests confirmed that domestic wastewater treatment plants effectively reduce the level of toxicity. In all domestic waste water treatment plants, IC50 values for L. minor and LC50 values for T. platyurus were determined only at the influent, as neither inhibition nor mortality higher than 50% compared to the control group was achieved at the effluent. In L. minor, the growth inhibition values (IC50) on the influent were in the range of 9.39–43.99 ml/l, in T. platyurus the LC50 values in the influent were determined in the range of 5.6–20 ml/l. However, the results of the chemical analysis revealed that none of the water from the domestic treatment plants selected by us is suitable for discharge into groundwater or surface water, as they exceed the limit values set in government regulations.
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Fotochemická degradace hypolipidemika atorvastatinu s využitím heterogenní fotokatalýzy.
VONEŠOVÁ, Adéla
The Bachelor s thesis is focused on hypolipidemic drugs statins and their representative atorvastatin. In the theoretical part of the thesis, the function of statins is introduced, studies about statins toxicity for freshwater organisms are presented, fundamentals of heterogeneous photocatalysis are explained and examples of usage of the method for pollutants degradation are given. Experimental part presents kinetics of atorvastatin degradation on TiO2 photocatalyst and a test of toxicity of atorvastatin photoproducts on a water plant (Lemna minor).
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Statiny - jejich použití, mechanismus účinku, vedlejší efekty. Atorvastatin a možnosti jeho fotochemické degradace v přírodních vodách.
PETRÁŇOVÁ, Pavla
The Bachelor´s thesis deals with a group of hypolipidemic drugs, statins. In the theoretical part, their development, characteristics, mechanism of action and side effects are presented. The experimental part is focused on one representant of statins, atorvastatin, its possible photochemical degradation in aqueous solution under light conditions relevant to natural waters, and a potential toxic effect of atorvastatin itself and of the mixture of products of atorvastatin photochemical degradation on a water plant Lemna minor.
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Evaluation of the efficiency of root treatment plants via ecotoxicity tests
Konečná, Kateřina ; Procházková, Petra (referee) ; Zlámalová Gargošová, Helena (advisor)
This bachelor thesis focuses on the evaluation of the efficiency of root treatment plants using appropriately selected ecotoxicity tests. For these purposes, ecotoxicological tests in an aquatic arrangement were used, specifically with the planktonic organism Thamnocephalus platyurus and the plant Lemna minor. The evaluation was performed at a municipal root treatment plant, which treats water for 955 equivalent inhabitants. The results of tests with T. platyurus confirmed that the root treatment plant sufficiently relieves acute toxicity of water. LC50 values were determined for two samples, 213.71 ml / l for the inflow and 388.20 ml / l for the settling tank. At the outflow, these values could no longer be determined due to the low ecotoxicological effect of the water thus treated. For tests with L. minor, IC50 values were also calculated for the first two samples. For Sample No. 1, the IC50 value was calculated to be 311.25 ml / l, for Sample No. 2 to 963.35 ml / l. However, on the basis of tests of direct toxicity of waters with L. minor, it is possible to confirm even in the case of this organism, a good cleansing effect of this root treatment plant.
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Spectrophotometric determination of chlorophyll
Rábová, Petra ; Vavrčíková, Veronika (referee) ; Řezáčová, Veronika (advisor)
This bachelor thesis is focused on determination of chlorophyll in leaves by the method of extraction spectrophotometry. The theoretical part provides information about photosynthesis, stress factors or plant adaptation, photosynthetic pigments (especially chlorophyll-a) and application of spectrophotometry and other methods of chlorophyll determination. The experimental part is focused on the application of the ecotoxicological test and, above all, it is evaluation by two independent methods. Specifically, it was determination of biomass growth inhibition and newly spectrophotometrical determination of chlorophyll mass. The results correlate with each other, therefore, the determination of chlorophyll seems to be an appropriate extension of usual evaluation methods of ecological tests.
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