|
|
The development of swine B cells and the role of gama delta T lymphocytes in immunization of naive immune system.
Štěpánová, Kateřina
Thesis summary The process of B cell lymphogenesis in swine remains uncertain. Some reports indicate that pigs belong to a group of animal that use ileal Peyers's patches (IPP) for the generation of B cells while others point to the possibility that the bone marrow is functional throughout life. The functional subpopulations of B cells in swine are also unknown. Together with other ruminants, and also birds, γδ T cells in swine may account for >70% of all T cells which is in apparent contrast with humans and mice. The purpose of this thesis was to address these discrepancies and unresolved issues. The results disprove the existing paradigm that the IPP is primary lymphoid tissue and that B cells develop in IPP in an antigen-independent manner. On the other hand, it shows that bone marrow is fully capable of B cell lymphogenesis and remains active at least for the same period of time as it had been speculated for the IPP. This thesis also identified functionally different subsets of porcine peripheral B cells, and shows that CD21 molecules can be expressed in differential forms. Finally, this thesis identifies two lineages of γδ T cells that differ in many functional and phenotype features. This finding may explain why γδ T cells constitute of minority of lymphocytes in circulation of humans and mice.
|
|
|
The development of swine B cells and the role of gama delta T lymphocytes in immunization of naive immune system.
Štěpánová, Kateřina ; Šinkora, Marek (advisor) ; Macela, Aleš (referee) ; Faldyna, Martin (referee)
Thesis summary The process of B cell lymphogenesis in swine remains uncertain. Some reports indicate that pigs belong to a group of animal that use ileal Peyers's patches (IPP) for the generation of B cells while others point to the possibility that the bone marrow is functional throughout life. The functional subpopulations of B cells in swine are also unknown. Together with other ruminants, and also birds, γδ T cells in swine may account for >70% of all T cells which is in apparent contrast with humans and mice. The purpose of this thesis was to address these discrepancies and unresolved issues. The results disprove the existing paradigm that the IPP is primary lymphoid tissue and that B cells develop in IPP in an antigen-independent manner. On the other hand, it shows that bone marrow is fully capable of B cell lymphogenesis and remains active at least for the same period of time as it had been speculated for the IPP. This thesis also identified functionally different subsets of porcine peripheral B cells, and shows that CD21 molecules can be expressed in differential forms. Finally, this thesis identifies two lineages of γδ T cells that differ in many functional and phenotype features. This finding may explain why γδ T cells constitute of minority of lymphocytes in circulation of humans and mice.
|
|
|
Cadaveric bone marrow transplantation: effects of hypoxia and metabolic starvation on mouse hematopoietic stem cells
Linhartová, Jana ; Šefc, Luděk (advisor) ; Průcha, Miroslav (referee) ; Šinkorová, Zuzana (referee)
Objectives: Hematopoietic stem cell transplantation (HSCT) is a widely used method for treatment of hematological disorders and some other diseases. However, sometimes a suitable donor of hematopoietic stem cells (HSC) is not found for a patient. Because HSC have been described as cells with low proliferative and metabolic activity, their tolerance to the lack of oxygen or metabolic substrates may be assumed. In this study, we explored cadaveric bone marrow as an alternative source of HSC for HSCT, using a mouse experimental model. In addition, the effect of in vitro metabolic inhibition and short-term in vitro storage (1 - 4 days) on functional properties of mouse HSC was investigated. Methods: C57Bl/6 mice (wild-type or p53-/- ) were used in the experiments. To explore cadaveric HSC, bone marrow (BM) was left in intact femurs at 37řC, 20řC and 4řC under the conditions of ischemia. The bone marrow cells were harvested after defined time periods ranging 0 - 48 hours. For metabolic inhibition, the electron transport chain inhibitor potassium cyanide (KCN) and inhibitor of glycolysis 2-deoxy-D-glucose (2-DG) were used in vitro. To determine the impact of ischemia, metabolic inhibition, or in vitro storage on transplantability of HSC, the competitive repopulation assay using Ly5.1/Ly5.2 congenic model...
|
|
|
The Specific of Nursing Care in Patient Undergoing the Examination of Bone Marrow
ČOUDKOVÁ, Marie
Haematology is a medical field. It is the study ofblood cells. It studies blood cells from the point of their morphology, quantity and function. The clinical haematology focuses on the irregularities in a patient´s blood and studies how the irregularitiesdiffer from the patient´s normal physiological condition. Due to this work haematology can help with diagnosis of other diseases. In haematology many investigative methods are used. One of them is aspiration and biopsy of bone marrow which is a hematopoietic tissue daily producing a large number of blood cells. Unless the formation of blood cells is sufficient enough, the disabled person may be at risk of their life. When the function of bone marrow is affected the patient may be impinged by serious infections, anemia risk or bleeding tendency. Bone marrow is examined for suspicion of haematological or oncology illnesses. Therefore, it has a very important rolein diagnosis of these. It is performed bya prolonged fever, mainly among the people who have a bad immune system. For blood collection are used investigative methodswhich consist ofaspiration and percutaneous bone marrow biopsy. These physical examinations provide some pieces of information about the condition and the ability of the blood cells´ production of bone marrow. Very important part of this physical examination is a specific nursing care. This bachelor´s thesis is focused on the specific nursing care of the patients who undergo the examinations of bone marrow. This thesis mainly includes the methods of bone marrow harvest, how the patients are informed about the examination of bone marrow,the patients´ nursing care after the operation and the role of a nurse at the haematological ward. The introduction of the theoretical part involves physiology of bone marrow and the study of haematology. The other parts discuss indication for bone marrow examination, haematological and oncological illnesses, bone marrow aspiration or percutaneous biopsy of bone marrow. The large part of the thesis deals with bone marrow aspiration, the process of the operation and the nursing care. It also focuses on percutaneous biopsy of marrow bone where the meaning of the examination and the process of bone marrow harvest operation are explained. At the end of the theoretical part a collection of hematopoietic stem cells from particular peripheral blood in mentioned. The aim of the bachelor thesis was to find out certain specifics of nursing care of the patients who undergo the examination of bone marrow. The following research questions were selected: What are the specifics of nursing care of the patients who undergo trepanobiopsy? What are the specifics of the patients who undergo aspiration (sternal puncture)? What are a preparation and assistance of a nurse during the examination of bone marrow? Which roles does the nurse have during the examination of bone marrow? How are the patients informed about the bone marrowexamination? The practical part includes results from a quantitative survey which was a semi-structured interview. The resulting interviews were written and then were processed by a certified coding technique. The research group comprised six nurses working in the haematological ward and six patients who have undergone the examination of bone marrow. Regarding the gathered results, an information brochure was put forward as a part of the thesis (see Annex number 5) and was oriented especially at patients undergoing the bone marrow examination. The brochure will be proposed for practical use at haematological wards.
|
|
| |
|
|
Effect reduced levels of vitamin B12 and folic acid on the morfology of erythrocytes
POPELOVÁ, Michaela
The morphology of erythrocyte is an important diagnostic element. Changes in morphology can point to certain diseases. Among various morphological features of erythrocytes the automatic analyzers commonly examine their size and stainability. The size of erythrocyte is ranging between 6.7 and 7.7 mmicrometrů. The smaller erythrocytes are called microcytes while bigger ones are called macrocytes. The size of erythrocytes is assessed by MCV (Mean Cell Volume), which indicates the average corpuscular volume. The supply of substances such as proteins, vitamins and minerals is important for proper development and metabolism of erythrocytes. Especially iron, vitamin B12 and folic acid are substances that affect the morphology of the erythrocyte. Deficiency of these substances leads to morphological changes in the blood picture. The deficiency of iron is presented in the peripheral blood like microcytosis (MCV <80 fl). The macrocytosis is the opposite change, which means deficiency of vitamin B12 and folic acid (MCV> 97 fl). This change is the main theme of project. As a result of deficiency of vitamin B12 and folic acid, changes in bone marrow are formed in addition to changes observed in the peripheral blood. Deficiency of vitamin B12 causes the megaloblastic reconstruction of bone marrow. This reconstructed bone marrow differs from normoblastic bone marrow by size, shape and and the gap between the cytoplasm and nucleus maturation. The retardation of nuclear maturation is caused by a suppressed formation of DNA related to the above mentioned deficiency of vitamin B12. Megaloblastic anemia and pernicious anemia are other hematological diseases caused by deficiency of vitamin B12. These diseases are at work also mentioned. The causes, which lead to a lack of both vitamins, are variable, for instance their insufficient food intake, or a defect in their assimilation. At present automatic analyzers offer a full range of tested parameters of blood cells. The samples were examined by an automatic analyzer Coulter LH 750, which operates on the principle of impedance. The value of MCV analyzer calculates using the software as the average of all measured impedance pulses. The macrocytosis was presented in 4% of the total number of samples examined for levels of vitamin B12. In 52% of samples deficient values of B12 (< 150 ng/l) content were observed. The macrocytosis was present in 7.5% of the total number of samples examined for level of folic acid. The deficit values of folic acid (< 3,1 microgram/l) were observed in 38% of all cases. At increased values of MCV 27% deficiency of vitamin B12 and 33% deficiency of folic acid was found. This work confirmed the link between lack of vitamin B12 and folic acid and the subsequent development of morphological changes. The deficiency in vitamin B12, in folic acid or in both vitamins occurred at high MCV.
|
|
| |
|
| |
|
|
Nursing of children on a transplant unit
HERKUCZOVÁ, Lenka
Oncological disease is very stressful and worrying for everyone. In the second half of 20th century, tumor disease became the second most frequent cause of death of children and young adults. They bring a major intervention to the child´s life, to the life of his or her family and to the lives of people close to the child. The research was proceeded on transplant unit and standard unit in Children´s Cancer Hospital. The objective of the thesis was to find out the specifics of the care on the transplant unit and based on obtained results determine actions that could make the care for children in the transplant unit more pleasant and improve the quality of the care. The objectives were fulfiled. Based on the research four hypothesis were formed.
|
|
| |
guest ::
