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Study of chicken antibodies against viral S protein of SARS-CoV-2
Křížová, Tereza ; Hodek, Petr (advisor) ; Svášková, Dagmar (referee)
In the beginning of the year 2020, the WHO declared a pandemic in relation with spreading of a new infection. The Covid-19 disease is mainly manifested by respiratory problems caused by the SARS-CoV-2 virus. It enters the host cell through the ACE2 receptor, to which it is bound by the RBD domain of the surface "spike" protein. A possible way of protection against viral infection is vaccination, e.g. with an mRNA vaccine, which induces the production of a "spike" protein to invoke an immune response and antibody production. Another possible alternative for protection is passive immunotherapy. In this thesis, antibodies from chicken eggs were chosen, which are extensively used in the prevention of various viral and bacterial diseases. They are characterized by high binding affinity to antigens, they are economically inexpensive and are considered a rapid, simple and safe method of passive protection. Antibodies IgY were prepared from egg yolks of chickens vaccinated with an mRNA vaccine encoding a "spike" protein. The reactivity of the prepared antibodies with the whole "spike" protein (expressed in insect cells) was tested by ELISA, demonstrating the ability of the antibodies to bind to the antigen with high affinity. Subsequently, their ability to interact with the RBD domain itself (expressed in...
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Health and social issues of vaccination
OLIVOVÁ, Vladimíra
The system of vaccination in the Czech Republic has a long tradition and the protection of public health and maintenance of a high immunization coverage in the population have always been a priority. The main goal of this bachelor's thesis is to perform an analysis of the morbidity levels of infectious diseases against which voluntary or mandatory vaccination is available in children and adults and of their social and health impacts, provide a rationale for vaccination and demonstrate the consequences of vaccine denialism. The theoretical part of the bachelor's thesis is based upon the analysis of scientific literature and represents a comprehensive introduction to the concept of vaccination, including a brief overview of the course, consequences and current spread of selected infectious diseases against which vaccination is available. The practical part of the bachelor's thesis is a combination of a secondary data analysis and quantitative research investigation utilizing the method of anonymous questionnaires, aimed at uncovering the opinions of citizens on the vaccination of children and adults. Three hypotheses were formulated. The H1 hypothesis that "The opinions of respondents concerning vaccination will differ based on their age", The H2 hypothesis that "Respondents with tertiary education have more information on the course and potential consequences of infectious diseases which can be vaccinated against" and The H3 hypothesis that "Younger respondents make use of the subsidization of non-mandatory vaccination provided by the insurance companies more often". The issue of vaccination has become an often-discussed topic in the recent years, in particular in relation to vaccination mandates. Vaccination objectors and organized anti-vaccination movements have begun to appear, resulting in undue emphasis being placed on detrimental side effects of vaccination, while ignoring the risks of infectious diseases and their health and social impacts.
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