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Software FLIM system with pulse white light laser in confocal microscopy
Grund, Pavel ; Odstrčilík, Jan (referee) ; Čmiel, Vratislav (advisor)
The theoretical part of this master's thesis is focused on research of confocal microscopy and FLIM method. There are a principles and types of confocal microscopy and the use of broad-spectrum laser as a basic light source of these microscopes. It gives what the FLIM method and its use not only in cell biology. The practical part thesis includes the acquisition of three sets of fluorescence intensity images with use of applications tunable pulsed laser, function TimeGate and detection of hybrid detectors. For practical elaboration of this thesis is in the software Fiji created a plugin, which is the source code in the Java programming language. The types of plugins and their uses are described in the third chapter of the thesis. This plugin including the graphical user interface in the form of the dialog box, proceses the fluorescence intensity images and creates a graphical representation of data showing the fluorescence lifetime, so called pseudocolor map.
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Secessionist trends in the Southern Pacific region within geopolitical context
Křiklánová, Michaela ; Riegl, Martin (advisor) ; Miková, Ivana (referee)
The bachelor's thesis "Secessionist trends in the Southern Pacific region within geopolitical context" focuses on secessionist movements, which emerged in the Southern Pacific region during the second half of the twentieth century and in the beginning of the twenty-first century. It deals with the circumstances of the formation, development and outcome of these movements in regional context. The secessionist movements are being characterised geographically, economically and socially in case studies and the preconditions of formation of these movements are identified according to John R. Wood theory. The thesis aims to describe the development and result of those movements.
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Software FLIM system with pulse white light laser in confocal microscopy
Grund, Pavel ; Odstrčilík, Jan (referee) ; Čmiel, Vratislav (advisor)
The theoretical part of this master's thesis is focused on research of confocal microscopy and FLIM method. There are a principles and types of confocal microscopy and the use of broad-spectrum laser as a basic light source of these microscopes. It gives what the FLIM method and its use not only in cell biology. The practical part thesis includes the acquisition of three sets of fluorescence intensity images with use of applications tunable pulsed laser, function TimeGate and detection of hybrid detectors. For practical elaboration of this thesis is in the software Fiji created a plugin, which is the source code in the Java programming language. The types of plugins and their uses are described in the third chapter of the thesis. This plugin including the graphical user interface in the form of the dialog box, proceses the fluorescence intensity images and creates a graphical representation of data showing the fluorescence lifetime, so called pseudocolor map.
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