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Identification of lactic acid bacteria in fermented dairy products using amplification methods
Tycová, Martina ; Rittich, Bohuslav (referee) ; Španová, Alena (advisor)
Polymerase chain reaction (PCR) is molecular diagnostic method which allows the identification of lactic acid bacteria used in food industry. In this work species-specific PCR primers (targeted on highly conserved 16S rDNA region) were used for identification of bacteria of species Streptoccocus thermophilus in 10 randomly commercially accessible fermented milk products and for identification of species Streptococcus thermophilus in 25 lyophilisates collected in Culture Collection of Dairy Microorganisms Laktoflora (CCDM, Tábor, Czech Republic). The PCR products (968 bp) were detected using electrophoresis in 1,2 % agarose gel. Bacterial DNA was isolated from crude cell lysates by magnetic carriers P(HEMA co GMA) containing carboxyl groups. DNA was reversibly bind on their surface in the presence of high concentrations of poly(ethylene glycol) (PEG 6000) and sodium chloride. Phenol extraction of DNA was used as control. Streptococcus thermophilus strains were identificated using PCR in all analysed samples.
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Fermented foods as a part of today's diet
Sochůrková, Veronika ; Pejšová, Hana (advisor) ; Chrpová, Diana (referee)
This bachelor thesis is devoted to fermented foods, which play an important role in human nutrition around the world. These include foods such as fermented milk products, fermented meat and fish products, fermented cereals, legumes, fermented beverages and kvass pastries. The theoretical part of the thesis focuses on the types and nutritional significance of individual groups of fermented foods. It also addresses the health aspects of the gut microbiome and the potential risks associated with the consumption of fermented foods. The aim of the work in the practical part was to determine the popularity and frequency of consumption of the most common fermented foods using a questionnaire survey. The results showed that the consumption of sour-milk products, especially yogurts, is very popular and most respondents include these foods in their diet several times a week. Fermented vegetables, especially sauerkraut, are also very popular. Most respondents are also interested in the topic of probiotics, and more than half of the respondents have tried homemade fermented foods. keywords: fermented foods, probiotics, fermented milk products, fermented vegetables, fermented legumes, fermented cereals, fermented beverages, kvass pastries
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Identification of lactic acid bacteria in fermented dairy products using amplification methods
Tycová, Martina ; Rittich, Bohuslav (referee) ; Španová, Alena (advisor)
Polymerase chain reaction (PCR) is molecular diagnostic method which allows the identification of lactic acid bacteria used in food industry. In this work species-specific PCR primers (targeted on highly conserved 16S rDNA region) were used for identification of bacteria of species Streptoccocus thermophilus in 10 randomly commercially accessible fermented milk products and for identification of species Streptococcus thermophilus in 25 lyophilisates collected in Culture Collection of Dairy Microorganisms Laktoflora (CCDM, Tábor, Czech Republic). The PCR products (968 bp) were detected using electrophoresis in 1,2 % agarose gel. Bacterial DNA was isolated from crude cell lysates by magnetic carriers P(HEMA co GMA) containing carboxyl groups. DNA was reversibly bind on their surface in the presence of high concentrations of poly(ethylene glycol) (PEG 6000) and sodium chloride. Phenol extraction of DNA was used as control. Streptococcus thermophilus strains were identificated using PCR in all analysed samples.
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Consumer preferences of selected milk products
ČÁSENSKÁ, Julie
The aim of this thesis was to evaluate the offer of fermented dairy products in the market network, and evaluate the favourite flavours of yogurts among university students. Market research of fermented dairy products was conducted in 3 hypermarkets, all the products in the offer were recorded. A simple anonymous questionnaire was created in order to obtain the necessary data about the popularity of each flavour among university students. The questionnarie was presented to a selected group of university students in the age of 20-35 years and it was completed by a total of 400 respondents. The results showed that the most popular yogurt is the one with pieces of fruit, the most popular flavour is strawberry. Banana flavour is the least favorite flavour among the consumers. The survey also showed that the most important criterion when choosing a yogurt is the producer and price.
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The inclusion of fermented dairy products in the diet of secondary school
VOLMANOVÁ, Miloslava
The theoretical section of the Bachelor Paper deals with the production of milk, kinds of milk and the processes applied to produce milk. Special attention is paid to the Fermented Milk Products (further only FMP), their types and processes of manufacture. The practical section surveys the consumption of milk and FMP, paying also attention to the general awareness of FMP among secondary-school students within the Czech Republic. The last section concentrates on the physiology of digestive tract, its structure, and on the benefits that fermented dairy products may have for human health.
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Probiotic bacteria in dairy products
KORANDOVÁ, Květa
Probiotic microorganisms are live organisms that facilitate optimal composition of intestinal flora. The thesis deals with the positive influence of probiotic microorganisms on human health. It describes the most frequently used bacteria family, which includes Lactobacillus, Lactococcus, Streptococcus and Bifidobacterium. The thesis also deals with health, microbiologic and technological requirements necessary for probiotic effectiveness. It offers an overview of characteristics of products containing probiotic microorganisms, which include pharmaceuticals and selected functional food. In the food group, the thesis particularly focuses on fermented milk products.
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Sensory evaluation of selected milk products
HALÁMKOVÁ, Eliška
The aim of this thesis was carried out using one of the methods of analysis of sensory evaluation of selected dairy products. Based on the proposed questionnaire survey carried out simultaneously on consumption and preference for selected commodities. The thesis was part of the project ECOP CZ.1.07/2.3.00/09.0081: "Comprehensive training of human resources in the dairy."
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