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Preservative systems for natural cosmetics
Koblasová, Dana ; Veselá, Mária (referee) ; Zemanová, Jana (advisor)
The aim of this bachelor thesis is to evaluate the antimicrobial activity of naturally derived preservatives against selected microorganisms. Theoretical part is focused on description of the most used natural, naturally derived and synthetic preservatives, on the safety of preservatives and finally, the issue of contamination of cosmetic products by microorganisms. In experimental part, antimicrobial activity of 4 naturally derived preservatives was evaluated against selected microorganisms, which were bacteria Micrococcus luteus, Kocuria rosea, Bacillus cereus, Bacillus subtilis, Serratia marcescens and one yeast Candida vini. The tested preservatives were from MinaSolve company - E-LEEN Green A, which contains pentylene glycol and phenylpropanol, E-LEEN Green OR, which is composed of citrus extracts, citric acid, ascorbic acid and pentylene glycol, Green B, which contains benzoic acid, sodium benzoate and pentylene glycol and Hexam+, which is a mixture of pentylene glycol and hexamidin diisethionate. Antimicrobial activity was evaluated by agar diffusion method. The highest antimicrobial activity showed Hexam+, the lowest E-LEEN Green OR. E-LEEN Green A and Green B did not show any antimicrobial activity against any microorganism in the 1% tested concentration. The measured values of the inhibition zones show that the most resistant microorganisms from all the tested in this thesis are Bacillus cereus and Bacillus subtilis and the least resistant are Micrococcus luteus and Kocuria rosea.
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Silver based raw materials for cosmetic purposes
Čaková, Adriana ; Ďubašáková, Mária (referee) ; Zemanová, Jana (advisor)
The purpose of this bachelor’s thesis was a research about silver based cosmetic products and determination of antimicrobial activity on certain species of microorganisms by chosen silver based cosmetic products. Theoretical part is focused on the problematics of silver based cosmetic products, on their main purpose, physically chemical characteristics and toxicological profile. In the theoretical part is also mentioned problematics of legislative, mainly the safety and real effectivity of tested cosmetic products. Experimental part was focused on determination of antimicrobial activity of chosen silver based cosmetic products. Two silver based cosmetic product were used to determined antimicrobial activity on selected microorganisms Bacillus subtilis, Bacillus cereus, Micrococcus luteus, Kocuria rosea, Serratia marcescens a Candida vini. From result it is apparent, that both of used Cosmetic product showed antimicrobial activity, but not on every used microorganism.
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Evaluation of the stability of cosmetic products
Kunertová, Aneta ; Pavelková, Renata (referee) ; Márová, Ivana (advisor)
This bachelor thesis deals with methods and parameters of evaluation of the stability of cosmetic products and application of selected tests on group of products. In the theoretical part, there are described basic types of cosmetic products. Definition of stability and characterization of two main testing types – long-term and accelerated are introduced. Selected tests are described in more detail. Another part deals with group of parameters categorized according to physical, chemical, sensory and microbiological properties observed during testing. Last parts are concentrated on influence of packaging on product stability and on evaluation. The experimental part focuses on preparation of cosmetic emulsion, its stability testing and comparison with 17 commercially available products. Among accelerated tests the cyclic test, thermal test, centrifugal test and microbial stress test are performed. It was determined that commercially available peeling C + citrus Planeta Organica has the highest stability. Satisfactory results were observed with the prepared emulsion.
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New trends in the use of probiotics in cosmetic products and their characterization
Dvořáková, Monika ; Němcová, Andrea (referee) ; Trachtová, Štěpánka (advisor)
Probiotic products have always been, in the form of various dairy products, a part of our diet. In recent years, they also became prominent in the pharmaceutical sector, where they can often be found as food supplements, and in the cosmetic sector, where they are being added to intimate hygiene and skin care products. The first part of the thesis consists of conducting a theoretical research in the field of probiotics, microbiome and other possibilities of where we can find probiotic organisms in cosmetic products. Further, a part of the thesis is devoted to methods of cultivating and isolating bacterial DNA from the following products: Benton, Mechnikov, La Rosche-Posay creams and Benton and Neogen masks, in sufficient quality for subsequent PCR amplification. DNA was isolated using magnetic carriers, a commercial kit, and phenol extraction. The presence of bacterial DNA was proved by a q-PCR analysis using genus-specified primers of selected bacteria. Genus identification corresponded with the information declared by the manufacturer. To conclude, a PCR and propidium monoazide dye were used in combination for quantification of viable and dead cells, and a HRM analysis was performed.
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Evaluation of the stability of cosmetic products
Kunertová, Aneta ; Pavelková, Renata (referee) ; Márová, Ivana (advisor)
This bachelor thesis deals with methods and parameters of evaluation of the stability of cosmetic products and application of selected tests on group of products. In the theoretical part, there are described basic types of cosmetic products. Definition of stability and characterization of two main testing types – long-term and accelerated are introduced. Selected tests are described in more detail. Another part deals with group of parameters categorized according to physical, chemical, sensory and microbiological properties observed during testing. Last parts are concentrated on influence of packaging on product stability and on evaluation. The experimental part focuses on preparation of cosmetic emulsion, its stability testing and comparison with 17 commercially available products. Among accelerated tests the cyclic test, thermal test, centrifugal test and microbial stress test are performed. It was determined that commercially available peeling C + citrus Planeta Organica has the highest stability. Satisfactory results were observed with the prepared emulsion.
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Preservative systems for natural cosmetics
Koblasová, Dana ; Veselá, Mária (referee) ; Zemanová, Jana (advisor)
The aim of this bachelor thesis is to evaluate the antimicrobial activity of naturally derived preservatives against selected microorganisms. Theoretical part is focused on description of the most used natural, naturally derived and synthetic preservatives, on the safety of preservatives and finally, the issue of contamination of cosmetic products by microorganisms. In experimental part, antimicrobial activity of 4 naturally derived preservatives was evaluated against selected microorganisms, which were bacteria Micrococcus luteus, Kocuria rosea, Bacillus cereus, Bacillus subtilis, Serratia marcescens and one yeast Candida vini. The tested preservatives were from MinaSolve company - E-LEEN Green A, which contains pentylene glycol and phenylpropanol, E-LEEN Green OR, which is composed of citrus extracts, citric acid, ascorbic acid and pentylene glycol, Green B, which contains benzoic acid, sodium benzoate and pentylene glycol and Hexam+, which is a mixture of pentylene glycol and hexamidin diisethionate. Antimicrobial activity was evaluated by agar diffusion method. The highest antimicrobial activity showed Hexam+, the lowest E-LEEN Green OR. E-LEEN Green A and Green B did not show any antimicrobial activity against any microorganism in the 1% tested concentration. The measured values of the inhibition zones show that the most resistant microorganisms from all the tested in this thesis are Bacillus cereus and Bacillus subtilis and the least resistant are Micrococcus luteus and Kocuria rosea.
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Silver based raw materials for cosmetic purposes
Čaková, Adriana ; Ďubašáková, Mária (referee) ; Zemanová, Jana (advisor)
The purpose of this bachelor’s thesis was a research about silver based cosmetic products and determination of antimicrobial activity on certain species of microorganisms by chosen silver based cosmetic products. Theoretical part is focused on the problematics of silver based cosmetic products, on their main purpose, physically chemical characteristics and toxicological profile. In the theoretical part is also mentioned problematics of legislative, mainly the safety and real effectivity of tested cosmetic products. Experimental part was focused on determination of antimicrobial activity of chosen silver based cosmetic products. Two silver based cosmetic product were used to determined antimicrobial activity on selected microorganisms Bacillus subtilis, Bacillus cereus, Micrococcus luteus, Kocuria rosea, Serratia marcescens a Candida vini. From result it is apparent, that both of used Cosmetic product showed antimicrobial activity, but not on every used microorganism.
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