National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Raman tweezers for sorting of living cells
Pilát, Zdeněk ; Ježek, Jan ; Kaňka, Jan ; Šerý, Mojmír ; Jákl, Petr ; Zemánek, Pavel
We have developed an instrument for automatized analysis and sorting of living cells of unicellular algae and other micro-objects based on laser tweezers and Raman spectroscopy. The system comprises the Raman tweezers setup, special microfluidic chip, and a specialized software allowing image recognition, spectral analysis, and automated sorting functions. The resulting instrument allows non-destructive analysis of chemical properties of living cells and their automatic separation for further examination or cultivation.
Cell sorting in practise
Cimburek, Zdeněk ; Horváth, Ondřej ; Růžičková, Š. ; Fišerová, Anna
Cell sorting is used for prepare cell clones, functional studies, clonal analyzis, expression studies. We are using FACS sort for positive and negative sorting, for single cells cloning and in some cases is used immunomagnetic presort

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