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Vliv délky ekvilibrace na motilitu spermií býků po rozmrazení
KRÁTILOVÁ, Karolína
The aim of the study was to monitor the change in motility of bull sperm post-thawing depending on the time of equilibration before freezing of insemination doses. The percentage of progressive sperm activity post-thawing was determined from the average progressive sperm activity after collection. The evaluation was performed on 6 bulls, approximately the same age, reared under the same conditions at the insemination station. 15 ejaculates were taken from each bull and subsequently processed into insemination doses, which were divided into four groups according to the length of equilibration: 1, 3, 8 and 24 hours. Equilibration was performed in a refrigerator at 5 ° C. The doses were then frozen and evaluated after one week of storage in liquid nitrogen. The mean values of the input parameters were: progressive motility at sampling was 74.63 ? 9.07, the amount of ejaculate 10.08 g and the density 0.91x 106 ml-1 sperm. The results document higher progressive sperm motility with longer equilibration times (8 and 24 hours) as opposed to shorter times (1 and 3 hours) (p <0.01). The best procentual progressive motility was recorded at the equilibration time of 8 hours, when it was 58.74 ? 13.92 % of the initial value (43,84 % of real progresive aktivity). When evaluating the progressive motility of bull sperm after thawing, statistical differences were observed between bulls (p <0.05). Determination of the progressive motility of bull sperm is important in terms of assessing bull fertility. The equilibration time is only one of many factors influencing the quality of the produced insemination doses.
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Aggregation of bull seminal plasma protein
Boháček, Hanuš ; Liberda, Jiří (advisor) ; Hýsková, Veronika (referee)
Mammalian fertilization is a sequence of unique and fascinating events, during which seminal proteins are of crucial role. In case of bull (Bos taurus), proteins of seminal plasma (BSP), especially its major component PDC-109, are known to be in aggregated forms, but little is known about mechanism of forming aggregates and their biological function. In present thesis we discovered some interesting properties of PDC-109 and BSP proteins. We found that concentration of these proteins influences their aggregation state significantly, which can be of great biological importance. Separation of seminal proteins by size exclusion chromatography revealed three main fractions denoted I, II and III, with apparent molecular weights of Mr > 150 000, Mr = 30 000 and Mr = 13 000, respectively. In case of PDC-109, molecular weights of theese fractions were retained even after purification procedure, which implies very stable interactions in forming of aggregates. In addition, there was a difference in distribution of PDC-109 glycoforms among fractions, which can be related to the fact, that theese fractions have different sperm membrane binding patterns as we determined by fluorescence microscopy. However, further experiments are needed for better understanding this issue.
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Species-specific structural differences of mammalian sperm and function of their key proteins during fertilization.
Dobrodinská, Anna ; Frolíková, Michaela (advisor) ; Kuntová, Barbora (referee)
The fertilization is a process during which a male and a female gamete merge so that a new organism may come into being. The sperm-egg fusion is preceded by several essential processes, such as the capacitation, acrosome reaction, the sperm binding to the zona pellucida and oolemma, and membrane fusion of the gametes. Numerous proteins, which are located in both sperm and eggs, are major actors in controlling the listed, essential processes. During the process of fertilization these proteins fulfil one or more functions. In mammalian sperm, significant species-specific differences may be found both in their morphology and at the protein level. A complex understanding of species-specific distinctions in sperm structure and functions of key sperm proteins would contribute to a better insight into the process of fertilization, thereby enabling us to better diagnose and subsequently treat the causes of infertility in humans. This bachelor's thesis summarizes the current knowledge of sperm structure and its key proteins that has been acquired through the studies of the following model mammal species: bull, boar, mouse, and human. Further, this thesis brings an interspecific comparison between the studied species. Keywords: sperm, fertilization, acrosome reaction, capacitation, sperm proteins, bull,...
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Species-specific structural differences of mammalian sperm and function of their key proteins during fertilization.
Dobrodinská, Anna ; Frolíková, Michaela (advisor) ; Kuntová, Barbora (referee)
The fertilization is a process during which a male and a female gamete merge so that a new organism may come into being. The sperm-egg fusion is preceded by several essential processes, such as the capacitation, acrosome reaction, the sperm binding to the zona pellucida and oolemma, and membrane fusion of the gametes. Numerous proteins, which are located in both sperm and eggs, are major actors in controlling the listed, essential processes. During the process of fertilization these proteins fulfil one or more functions. In mammalian sperm, significant species-specific differences may be found both in their morphology and at the protein level. A complex understanding of species-specific distinctions in sperm structure and functions of key sperm proteins would contribute to a better insight into the process of fertilization, thereby enabling us to better diagnose and subsequently treat the causes of infertility in humans. This bachelor's thesis summarizes the current knowledge of sperm structure and its key proteins that has been acquired through the studies of the following model mammal species: bull, boar, mouse, and human. Further, this thesis brings an interspecific comparison between the studied species. Keywords: sperm, fertilization, acrosome reaction, capacitation, sperm proteins, bull,...
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The Jews in the Popes Documents of 12. - 14. century
Stehlíková, Terezie ; Arava-Novotná, Lena (advisor) ; Holubová, Markéta (referee)
UNIVERZITA KARLOVA V PRAZE HUSITSKÁ TEOLOGICKÁ FAKULTA Židé v papežských dokumentech 12. - 14. století The Jews in the Popes Documents of 12. - 14. century Diplomová práce Vedoucí práce: Autor: PhDr. Lena Arava-Novotná, Th.D. Bc. Terezie Stehlíková Praha 2016 Poděkování Ráda bych touto cestou vyjádřila poděkování PhDr. Leně Aravě-Novotné, Th.D. za odborné vedení, trpělivost a ochotu, kterou mi v průběhu zpracování věnovala při vedení mé diplomové práce. Dále bych také chtěla poděkovat všem, kdo mě v mém studiu podporovali. Prohlášení Prohlašuji, že jsem tuto diplomovou práci s názvem "Židé v papežských dokumentech 12. - 14. století" napsala samostatně a výhradně s použitím citovaných pramenů a literatury. V Praze dne Bc. Terezie Stehlíková Anotace Tato diplomová práce "Židé v papežských dokumentech 12. - 14. století" se zabývá přístupy papežů vůči židům, kteří žili v křesťanské společnosti. Cílem práce je nejen připomenout papeže a jejich buly, které se nějakým způsobem dotýkaly židů, ale také jmenovat církevní koncily a poukázat na jejich dopad na židovské obyvatelstvo. Součástí práce je také malé nastínění dějinných událostí, na které měly papežské buly svůj vliv. Annotation This thesis "The Jews in the Popes Documents of 12. - 14. century" deals with the attitudes of popes to the Jews who lived in a...
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Aggregation of bull seminal plasma protein
Boháček, Hanuš ; Liberda, Jiří (advisor) ; Hýsková, Veronika (referee)
Mammalian fertilization is a sequence of unique and fascinating events, during which seminal proteins are of crucial role. In case of bull (Bos taurus), proteins of seminal plasma (BSP), especially its major component PDC-109, are known to be in aggregated forms, but little is known about mechanism of forming aggregates and their biological function. In present thesis we discovered some interesting properties of PDC-109 and BSP proteins. We found that concentration of these proteins influences their aggregation state significantly, which can be of great biological importance. Separation of seminal proteins by size exclusion chromatography revealed three main fractions denoted I, II and III, with apparent molecular weights of Mr > 150 000, Mr = 30 000 and Mr = 13 000, respectively. In case of PDC-109, molecular weights of theese fractions were retained even after purification procedure, which implies very stable interactions in forming of aggregates. In addition, there was a difference in distribution of PDC-109 glycoforms among fractions, which can be related to the fact, that theese fractions have different sperm membrane binding patterns as we determined by fluorescence microscopy. However, further experiments are needed for better understanding this issue.
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Duty cycle bull terrier type
TÖRÖKOVÁ, Jacquelina
This work deals with the history of born, origin, domestication of the dog and various types of bull terriers. Pointing at the crossing of the Bulldog with a black terrier. This work also deals with the different types of dogs Bull, their character, description. This work answers the question, ?Why the pit bull is not dangerous to people", evaluates conflict situations, and shows how conflicts can occur. It mentions the current differences and cons of each breed. It places an attempt to create a new giant of American Bully and also eveluates the unknown type of gulterier. This paper introduces the basics of breeding dogs from purchasing the puppy to its recurring development, communication, behavior and training. The work refers to disease prevention, training and legal responsibility of breeding and finally recommends the use of Terrier ? the type of bul for practice incl. sports use.
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