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The Development of HPLC-FLD Method for the Determination of the Bile Acids in the Bile of Mice
Kacerovská, Veronika ; Nesměrák, Karel (advisor) ; Bosáková, Zuzana (referee)
Aim of the master thesis was the development and the optimization of the HPLC method with fluorescence detection for the determination of the bile acids in the bile of mice. On the basis of literature, the HPLC-FLD method has been proposed and optimized for the quantification of eight bile acids (cholic acid, glycocholic acid, deoxycholic acid, glycodeoxycholic acid, chenodeoxycholic acid, glycochenodeoxycholic acid, α-muricholic acid, and λ-muricholic acid) in the biological material. 1,2-benzo-3,4-dihydrocarbazole-9-ethyl-p-toluenesulfonate is suitable derivatization reagent for labeling of the bile acids. The calibration dependences, limits of detection and quantification (in the range of 0.88-1.78 μmol dm -3 ) for all bile acids are measured. In the biological sample five bile acids have been successfully identified and then determined (cholic acid, glycocholic acid, glycodeoxycholic acid, glycochenodeoxycholic acid, and α-muricholic acid). Key words: bile acids, HPLC, fluorescence detector, derivatization reaction, bile
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Linguistic picture of the particular somatisms in Czech
Škanderová, Adéla ; Janovec, Ladislav (advisor) ; Chejnová, Pavla (referee)
The bachelor thesis deals with a lingvistic picture of the world of the particular somatisms, namely the components of the digestive system (stomach, bile, gall bladder, heartburn, intestine). The theoretical part covers the term cognition, cognitive science and, logically, cognitive linguistics. Furthermore, it includes the indispensable and key topics which have to be taken into account to understand the concept of the linguistic picture of the world, i.e. conceptualization, categorization, metaphor, anthropocentrism etc. The practical part is interested in the real occurence of the somatisms in various language contexts. In particular, their occurence in collocations, phraseology and idiomatic expressions. The analysis is based on the profile theory and its image schema. The lingustic backround is supplemented with the psychosomatic knowledge because it appears to be a helpful science branch while completing and considering the possible connection between the occurence of somatism in the language of the specific culture and extralinguistic reality.
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