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Regulatory functions of the transmembrane adaptor protein NTAL in activation of mast cells
Tůmová, Magda ; Dráber, Petr (advisor) ; Vyklický, Ladislav (referee) ; Hašek, Jiří (referee)
Mast cells originate from hematopoietic pluripotent stem cells present in bone marrow. They do not circulate, but are spread throughout a body and reside in all vascularized tissues. Mast cells as a part of innate immune system are also able to influence adaptive immune system and are effectors in IgE-mediated allergic and inflammatory diseases. Mast cells possess high affinity receptor for IgE (FcεRI). Aggregation of the receptor triggers signaling cascades which lead to the release of preformed and de-novo synthesized effector molecules. Early phases of mast cell activation include tyrosine phosphorylation of numerous substrates and formation of signaling assemblies (also called signalosomes). Important structural components of the signalosomes are transmembrane adaptor proteins. One of them is the transmembrane adaptor protein NTAL (Non-T cell Activation Linker), which becomes rapidly tyrosine- phosphorylated upon FcεRI triggering and serves as a positive or a negative regulator of FcεRI signaling. To be able to study fine topography of NTAL and other plasma membrane components in mouse bone marrow-derived mast cells (BMMCs), we first developed a new method of plasma membrane sheets isolation from non-adherent cells. The method is based on adsorption of the cells to ultraclean glass cover-slips,...
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Outsourcing IS/ICT - formy, modely využití, trendy
Tůmová, Magda ; Voříšek, Jiří (advisor) ; Černý, Karel (referee)
Práce uvádí definici outsourcingu, jeho historii, důvody pro outsourcing a také možné nevýhody. Věnuje se informatickým zdrojům, procesům a službám. Uvádí dělení outsourcingu na outsourcing vývoje, provozu vlastnicví a odpovědnosti a na offshore a onshore outsourcing. Detailně popisuje rozdělení podle přemětu outsourcingu, kde jsou u jednotlivých modelů outsourcingu uváděny přínosy, možná rizika, kritické faktory a další specifika. Doporučuje jednotlivé kroky outsourcingu, které je dobré při jeho postupu dodržet. Obsahuje vlastní analýzu poskytovatelů outsourcingu v České republice. Nastiňuje trendy outsourcingu jak ve světě, tak v České republice.
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