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Cognitive Rehabilitatiton in Multiple Sclerosis Patients by an Occupational Therapist. Subtitle: Testing a New Application for Touch Devices.
Holeňová, Martina ; Novotná, Klára (advisor) ; Straková, Eva (referee)
BACHELOR THESIS ABSTRACT Name, Surname: Martina Holeňová Thesis supervisor: Mgr. Klára Novotná Thesis opponent: Title of the Bachelor Thesis: Cognitive Rehabilitation Cognitive Rehabilitation in Multiple Sclerosis Patients by an Occupational Therapist: Testing a New Application for Touch Devices Abstract: This bachelor thesis deals with cognitive rehabilitation in patients with multiple sclerosis (MS) from the perspective of an occupational therapist, who together with a psychologist or a speech therapist practise cognitive function training. The extent of cognitive functions is variable, occur mild to moderate disorders, therefore has cognitive rehabilitation in patients with MS great importance. There are many cognitive rehabilitation software available, but none of them is primarily focused on MS patients. The aim of this thesis is to evaluate feasibility of the new sofrware for cognitive rehabilitation in people with MS (called Kote). In bachelor thesis is used the research method of a written questionnaire survey and on the basis of the evaluation of the non - standardized questionnaire the authors will be provided with suggestions for further software upgrades. Another aim is to prepare instructions for every game and to assign the area of cognitive functions that is trained in the game. The thesis is...
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Identification and modeling of gene expression regulatory networks during streptomycetes germination
Straková, Eva ; Vohradský, Jiří (advisor) ; Stopka, Pavel (referee) ; Vondrášek, Jiří (referee)
Streptomycetes have been studied mostly as producers of antibiotics and for fundamentals of complex bacterial cell development. Here, transcriptomic and proteomic approaches were applied to systems study of Streptomyces coelicolor germination as a developmental transition from dormancy to the vegetative stage. The time dynamics of the gene expression levels represented by mRNA and intracellular protein accumulation and synthesis were measured throughout 5.5 h of germination at 13 time points by employing both DNA microarray and two-dimensional gel electrophoresis techniques. Using a numerical model of gene expression, genetic networks were reconstructed and functional groups of genes controlled by the sigma factors were identified. Modeling of the regulatory interactions provided a set of parameters allowing simulate kinetics of gene expression control among the sigma factors and their target genes. Particularly regulons of two sigma factors, SigR and HrdD, were identified. The analysis assigned their key role during the germination process. Analysis of global trends in the gene/protein expression revealed that the full capability of regulatory mechanisms responding to the environmental cues is reached within the first hour of germination, and identified the basic gene/protein functional groups...
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Study of factors influencing proton transport mediated by MntH protein.
Straková, Eva ; Urbánková, Eva (advisor) ; Žáčková, Markéta (referee)
The natural resistance-associated macrophage proteins (Nramp) form a family of secondary active transporters facilitating the transport of divalent metal ions (Mn2+, Fe2+, Cd2+, Co2+, Zn2+) across biomembranes. Bacterial proteins belonging to the Nramp family were describedas proton-dependent manganese transporters - MntH. MntH homolog from E. coli representing a model system for structure function relationship was used in this study. Metalinduced proton transport mediated by MntH was measured by means of changes in intracellular proton concentrations using pH sensitive fluorescent protein pHluorin. This approach does not allow proper estimation of real quantity of transported protons, since the internal pH is tightly regulated. The cellular buffering capacity was calculated from measured pH changes induced by addition of propanoic acid, in order to better quantify the transport. The changes in [H+] induced by cadmium were recalculated using buffering capacity for the wild type MntH and its single-point mutation N401G, known to influence intracellular pH. It was shown that the chosen approach significantly influences results, including the dependence of transport on external pH. Potential influence of cadmium to respiration was imitated by using inhibitors of respiratory chain (KCN, NaN3) in measurements...
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