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Electrochemistry of Flavonolignans and their Interactions with DNA and Proteins
Pyszková, M. ; Zatloukalová, M. ; Biedermann, David ; Křen, Vladimír ; Ulrichová, J. ; Ramešová, Šárka ; Sokolová, Romana ; Vacek, J.
Electrochemical oxidation of flavonolignans silybin, silydianin, silychristin and their 2,3-dehydroderivatives, was studied using ex situ and in situ cyclic and square wave voltammetry at pyrolytic graphite electrode. The pilot results presented here could be used for further investigation of mechanism of oxidation and reactivity of flavonolignans to DNA and proteins.
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Electrochemistry as a Tool for an Enzyme Characterization
Gál, M. ; Krahulec, J. ; Jiríčková, K. ; Sokolová, Romana ; Híveš, J.
Basic biochemical properties such as Michaelis constant (KM) and turnover number (kcat) of enzyme enteropeptidase was measured by electrochemical impedance spectroscopy. Both values determined by impedance measurements are in good agreement with those obtained by traditional spectroscopic techniques. Therefore one can suppose that electrochemical methods might be successful for such measurements also in the case where usual ones (UV-Vis, fluorescence spectroscopy) are not able to determine these enzyme characteristics.
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Electrochemical Study of Rhamnazin
Ramešová, Šárka ; Sokolová, Romana ; Degano, I.
The natural flavonoid compound rhamanzin (3,5,4’-trihydroxy-7,3’-dimethoxyflavone) is important bioactive compound with antioxidative, anti-allergic, and anti-inflammatory properties. The cyclic voltammetry is used for the electrochemistry behavior of rhamnazin. The determination of oxidation pathways is supported by the identification of degradation products using separation technique.
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Adsorption of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine on Au(111) and HOPG
Kocábová, Jana ; Sokolová, Romana
Atomic force microscopy (AFM) was used to study the formation of layers of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) on Au(111) and highly oriented pyrolytic graphite (HOPG) in microscopic level. Both substrates were found to support the DPPC layers. The nanoshaving technique was applied to determine the layer thickness. In addition, the influence of deionized water on the DPPC ordering at the substrates was studied by ex-situ and in-situ AFM intermittent contact mode measurements.
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Electrochemistry of Potential Eu MRI Complexes
Gál, Miroslav ; Sokolová, Romana ; Kielar, P.
Properties of the several potential contrast agents for magnetic resonance imaging (MRI) with Eu(III)/Eu(II) redox couple were investigated by means of the electrochemical methods. Cyclic voltammetry, phase sensitive AC voltammetry, and DC polarography were utilized to elucidate the mechanism of the reduction/oxidation of Eu ion in the presence of these compounds. Moreover, the stability constants of the Eu(II)-MRI agent complexes were also determined. Some suggestions on the chemical structure of the potential contrast agents for MRI are also made.
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