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Identification of bacterias from positive blood cultures using mass spectrometry
Jahnová, Aneta ; Paterová, Pavla (advisor) ; Scharfen, Josef (referee)
CHARLES UNIVERSITY IN PRAGUE Faculty of Pharmacy in Hradec Králové Department of Biological and medical Sciences Candidate: Bc. Aneta Jahnová Supervisor: MUDr. Pavla Paterová, Dept. of Clinical Microbiology FN Hradec Králové Consultant: primářka MUDr. Alena Ševčíková, Dept. of Clinical Microbiology FN Brno Title of diploma thesis: Identification of bacteria from positive blood cultures by mass spectrometry. Background: During processing positive blood culture at the Department of Clinical Microbiology, University Hospital Brno MALDI-TOF MS is used to for rapid and reliable identification cultured bacteria growing pure culture after five to six hours on solid culture media. The aim of this study was evaluated and compared methods for direct identification of the etiological agents from positive blood cultures using a kit SepsiTyperTM and test tubes with gel, which should decrease the time to obtain the result. Methods: In this study were tested 50 positive BacT/ALERT blood cultures. Preparation of microbial samples was carried out parallel using a kit SepsiTyperTM and collection test tubes with gel. In both methods was performed extraction with ethanol/formic acid and analyses on a Microflex LT system. Results: From fifty testing blood cultures were correctly identified by kit SepsiTyper 24 samples...
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Using of MALDI-TOF Mass Spectrometry and Other Methods for Identification of Microorganisms
Šafránková, Renáta ; Žemličková, Helena (advisor) ; Sedláček, Ivo (referee) ; Scharfen, Josef (referee)
Using of MALDI-TOF mass spectrometry and other methods for identification of microorganisms. Background MALDI-TOF MS (Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry) method for identification of microorganisms appeared not long ago. It is based on analysis of cell proteome. The method has undergone rapid development recently and it gradually complemented or even substituted classical routine biochemical methods. It became highly important part of microbiological laboratories. An increase in its use contributed considerably to starting of effective therapies timely and resulted in shortening of time needed for medical care. Numerous laboratory techniques, that are available nowadays, can be applied for more detailed characterization of isolated microbes, when it is necessary. The molecular-biological methods are of the most importance, namely polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), 16S rRNA gene sequence analysis or whole gene sequencing (WGS) analysis. Aims Four particular groups of infectious agents (Campylobacter spp., Plesiomonas shigelloides, Citrobacter spp., Staphylococcus intermedius/Staphylococcus pseudintermedius) were described and/or reidentified by MALDI-TOF MS and other methods (PCR, RFLP, extended biochemical...
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Identification of bacterias from positive blood cultures using mass spectrometry
Jahnová, Aneta ; Paterová, Pavla (advisor) ; Scharfen, Josef (referee)
CHARLES UNIVERSITY IN PRAGUE Faculty of Pharmacy in Hradec Králové Department of Biological and medical Sciences Candidate: Bc. Aneta Jahnová Supervisor: MUDr. Pavla Paterová, Dept. of Clinical Microbiology FN Hradec Králové Consultant: primářka MUDr. Alena Ševčíková, Dept. of Clinical Microbiology FN Brno Title of diploma thesis: Identification of bacteria from positive blood cultures by mass spectrometry. Background: During processing positive blood culture at the Department of Clinical Microbiology, University Hospital Brno MALDI-TOF MS is used to for rapid and reliable identification cultured bacteria growing pure culture after five to six hours on solid culture media. The aim of this study was evaluated and compared methods for direct identification of the etiological agents from positive blood cultures using a kit SepsiTyperTM and test tubes with gel, which should decrease the time to obtain the result. Methods: In this study were tested 50 positive BacT/ALERT blood cultures. Preparation of microbial samples was carried out parallel using a kit SepsiTyperTM and collection test tubes with gel. In both methods was performed extraction with ethanol/formic acid and analyses on a Microflex LT system. Results: From fifty testing blood cultures were correctly identified by kit SepsiTyper 24 samples...
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Neoclassical Realism and the Dynamics of Power in Eastern Asia: U.S. foreign policy toward China
Scharfen, Josef ; Karlas, Jan (advisor) ; Drulák, Petr (referee)
Diploma thesis "Neoclassical Realism and the Dynamics of Power in Eastern Asia: U.S. Foreign Policy toward China" is testing specific theoretical propositions of neoclassical realism on the evolution of U.S. foreign policy toward China from 1950 until present. This single case study uses congruence method and process-tracing to test the effect of intervening variables on the foreign policy deviation from neorealist proposition. Paper assesses theoretical progressivity of neoclassical realism and on this basis analyzes possible future evolution of Sino-American relations and in similar fashion presents suggestions to effective U.S. foreign policy.
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