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Depression and its risk factors
Sejkorová, Monika ; Rathouská, Jana (advisor) ; Kudláčková, Zděnka (referee)
Background: Thesis consists of theoretical summary of information which characterizes depression. Practical part consists of questionnaire study, whose goal is to determine the most important factors, which take part in growth of depression disease. As well to determine the effect of season on depression progress, the most common age when depression starts to develop, eventually some knowledge about a character and treatment of depression. Methods: Questionnaire study from November 2017 to April 2018, which consists of 22 questions for respondents from Czech Republic (850 respondents of whom 291 were diagnosed depression). Results: More than a half of respondents (53 %) diagnosed with depression have chosen two and more risk factors. Thus, depression appears based on combination of more factors. The most frequently are mentioned ill family relationships (20 %) and tragic event in life (20 %). It is also negatively influenced by season, mostly autumn (46 % of answers) and winter (33 % of answers). The most common age when the depression starts to appear is 11-20 years. Most frequently respondents were diagnosed moderate level of depression (60 %) and they have chosen to visit a doctor out of their own belief (57 %). The main signs are tiredness (18 %) and loss of interest and enjoyment of activities...
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Western blot analysis of selected oxidative stress markers in aorta of apoE/LDLR deficient mice
Čermáková, Martina ; Rathouská, Jana (advisor) ; Blažíčková, Kateřina (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Western blot analysis of selected oxidative stress markers in aorta of apoE/LDLR deficient mice Author of diploma thesis: Bc. Martina Čermáková Supervisor: PharmDr. Jana Rathouská, Ph.D. Background: The aim of this diploma thesis was to evaluate expression of selected oxidative stress molecules (SOD-3, iNOS, catalase, HO-1) in cardiac aorta and nitrate and nitrite levels in plasma in two groups of mice. These two groups of mice varied in genetic features - the monitored group was, compared to control group of mice, deficient for apolipoprotein E and LDL receptor. Methods: For the study, 2 months old male mice were fed a standard rodent diet. The males were divided into two groups - control (C57BL/6J) and monitored (apoE-/- /LDLR- /- ). Western blot method was used to evaluate and quantify iNOS, SOD-3, catalase and HO-1 molecules in the aorta. HPLC method was used to quantify nitrate and nitrite levels in plasma. Result: HPLC method did not show statistically significant differences in the nitrate and nitrite levels in plasma between the two groups of examined mice. Western blot analysis of aortic samples showed statistically significantly higher SOD-3 expression (208.39 %) and a significantly...
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Attitude of mothers to compulsory vaccination in children
Červinková, Pavla ; Rathouská, Jana (advisor) ; Konečná, Klára (referee)
Bc. Pavla Červinková Attitude of mothers to compulsory vaccination in children Charles University, Faculty of Pharmacy in Hradec Králové Specialist in laboratory methods The most effective prevention of serious infectious diseases is vaccination. In the Czech Republic, there is a compulsory vaccination against nine diseases, which have not been eradicated in the world yet. The topic of compulsory vaccination is currently a very topical and discussed issue. Objectives: Theoretical overview of the issue of compulsory vaccination in children, individual diseases, the possible risks of vaccination and available vaccines. The aim of the questionnaire study is to determine the percentage of mothers with a negative attitude to this issue and their reasons for their attitude. Methods: Questionnaire study within the Czech Republic. The questionnaire was intended for women - mothers of different age categories. Results: In the general public, there is a wide range of views on this issue. Most of mothers have a positive attitude towards compulsory vaccination. A significant part of mothers point to a bad legislation in the Czech Republic. They particularly criticize the vaccination scheme and the obligation of using combined vaccines, in which mothers often observe side effects. Conclusion: The questionnaire...
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Western blot analysis of selected inflammatory markers in aorta of apoE/LDLR deficient mice
Krejcarová, Jana ; Rathouská, Jana (advisor) ; Nachtigal, Petr (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of Diploma Thesis: Western blot analysis of selected inflammatory markers in aorta of apoE/LDLR deficient mice Author: Jana Krejcarová Supervisor: PharmDr. Jana Rathouská, Ph.D. Background: This thesis was focused on the detection and subsequent evaluation of the expression of adhesion molecules ICAM-1, VCAM-1 and COX-2 and P-selectin in mouse aortas. Control and monitored group of mice differed in the genetic material, where monitored group of mice had unfunctional genes for apolipoprotein E and LDL receptor (apoE−/− /LDLR−/− ). Methods: For the study, two months old male mice of the C57BL/6J strain were used. Males were divided into two groups - control and monitored with unfunctional receptor for low- density lipoprotein and for apolipoprotein E. Both groups were fed by a standard diet for rodents. For detection and subsequent quantification of the expression of the markers VCAM-1, ICAM-1, COX-2 and P-selectin, Western blot analysis was used. Levels of soluble ICAM-1 and P-selectin in plasma were assessed by Luminex®. Results: Western blot analysis showed no significant difference in the expression of VCAM-1, ICAM-1, COX-2 and P-selectin between both groups of mice. Using the...
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The recommended vaccination of the adult population in the Czech Republic
Nová, Petra ; Rathouská, Jana (advisor) ; Janďourek, Ondřej (referee)
Author: Petra Nová, DiS. Title: The recommended vaccination of the adult population in the Czech Republic Bachelor thesis Charles University, Faculty of Pharmacy in Hradec Králové Field of study: Medical Laboratory Technician Background: The aim is to refer the possibilities of recommended vaccines for adults and focus on the currently available vaccines on the Czech market. The partial aim of the work is to describe the importance of vaccination as such and partly to disprove the opinions of its uselessness. At the end, the work deals with data processing and evaluation of vaccines dispensing in two selected pharmacies. Methods: Statistical evaluation of vaccines dispensing in two private pharmacies in Jicin in 2014 - 2016. Evaluation includes the types of dispensed vaccines, gender and the age categories for which the individual vaccines are determined. Results: Concerning the dispensed vaccines, there were mainly vaccines against influenza, tick-borne encephalitis and viral hepatitis A. The most commonly prescribed vaccines were against the flu. In 2015, September-December period was recorded the largest number of these dispensed vaccines. Conversely, in 2016 there was the lowest interest in this vaccination. Persons between 45 - 60 years were the most represented age category for the flu...
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Western blot analysis of selected oxidative stress markers in aorta of apoE/LDLR deficient mice
Čermáková, Martina ; Rathouská, Jana (advisor) ; Blažíčková, Kateřina (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Western blot analysis of selected oxidative stress markers in aorta of apoE/LDLR deficient mice Author of diploma thesis: Bc. Martina Čermáková Supervisor: PharmDr. Jana Rathouská, Ph.D. Background: The aim of this diploma thesis was to evaluate expression of selected oxidative stress molecules (SOD-3, iNOS, catalase, HO-1) in cardiac aorta and nitrate and nitrite levels in plasma in two groups of mice. These two groups of mice varied in genetic features - the monitored group was, compared to control group of mice, deficient for apolipoprotein E and LDL receptor. Methods: For the study, 2 months old male mice were fed a standard rodent diet. The males were divided into two groups - control (C57BL/6J) and monitored (apoE-/- /LDLR- /- ). Western blot method was used to evaluate and quantify iNOS, SOD-3, catalase and HO-1 molecules in the aorta. HPLC method was used to quantify nitrate and nitrite levels in plasma. Result: HPLC method did not show statistically significant differences in the nitrate and nitrite levels in plasma between the two groups of examined mice. Western blot analysis of aortic samples showed statistically significantly higher SOD-3 expression (208.39 %) and a significantly...
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Western blot analysis of selected inflammatory markers in aorta of apoE/LDLR deficient mice
Krejcarová, Jana ; Rathouská, Jana (advisor) ; Nachtigal, Petr (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of Diploma Thesis: Western blot analysis of selected inflammatory markers in aorta of apoE/LDLR deficient mice Author: Jana Krejcarová Supervisor: PharmDr. Jana Rathouská, Ph.D. Background: This thesis was focused on the detection and subsequent evaluation of the expression of adhesion molecules ICAM-1, VCAM-1 and COX-2 and P-selectin in mouse aortas. Control and monitored group of mice differed in the genetic material, where monitored group of mice had unfunctional genes for apolipoprotein E and LDL receptor (apoE−/− /LDLR−/− ). Methods: For the study, two months old male mice of the C57BL/6J strain were used. Males were divided into two groups - control and monitored with unfunctional receptor for low- density lipoprotein and for apolipoprotein E. Both groups were fed by a standard diet for rodents. For detection and subsequent quantification of the expression of the markers VCAM-1, ICAM-1, COX-2 and P-selectin, Western blot analysis was used. Levels of soluble ICAM-1 and P-selectin in plasma were assessed by Luminex®. Results: Western blot analysis showed no significant difference in the expression of VCAM-1, ICAM-1, COX-2 and P-selectin between both groups of mice. Using the...
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Up-to-date antiretroviral therapy
Teichmann, Radek ; Jílek, Petr (advisor) ; Rathouská, Jana (referee)
2 Abstract Radek Teichmann Modern antiretroviral therapy Bachelor thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Health bioanalytics - Medical Laboratory Modern antiretroviral therapy is a form of treatment that suppresses the spread of the virus in the cell and in the case of some drugs prevents viral entry into the cell. The aim of this therapy is to restore the number of white blood cells to the original values. Combined antiretroviral therapy can effectively reduce HIV replication and slow down the progression of immunodeficiency. It will ensure a significant increase in life expectancy to HIV-infected patients. The Thesis discusses the treatment of diseases associated with HIV. The main objective is to create a comprehensive overview of the evaluation of drugs called antiretrovirals.
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Multiple sclerosis - laboratory aspects
Lhotáková, Vendula ; Jílek, Petr (advisor) ; Rathouská, Jana (referee)
56 22 Abstract Author: Vendula Lhotáková Title: Multiple sclerosis - laboratory aspects Bachelor work Univerzita Karlova v Praze, Farmaceutická fakulta v Hradci Králové Field of study: Medical laboratory technician, combined study form Background: Bachler work thesis is written as a literary review. The main effort is to point to the diagnosis of multiple sclerosis, to set out its main risk factors highlighted immunological background and last but not least, to find possible biomarkers of disease. Main findings: Multiple Sclerosis (MS) is an inflammatory auto-immune disease affecting especially the younger generation. When the disease causes damage to the myelin -protective layer of axons (neuronal projections), it is characterized by inflammatory lesions in the brain causing CNS dysfunction. MS history dates back to the 14th century , although the reversal in the diagnosis of the disease occurred only in the 20s. Diagnosis is difficult because symptoms of the disease are not uniform. With technological advances, however, the diagnosis is considerably improved. Nowadays, diagnostic processes are based on a particular variety of laboratory tests including Magnetic resonance imaging. Very useful is the test of the cerebro spinal fluid, as well as other markers of the disease. RS is determined genetically and...
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