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The impact of the pandemic on women in healthcare
Pospíšilová, Marie
Healthcare workers have become the new heroes, or rather heroines, during the pandemic, as 85% of healthcare workers in the Czech Republic are women. The crisis has further exposed the problems associated with the difficulty of reconciling work in the health sector and caring for one's own family. Almost a third of nurses have children under the age of 12. The closure of schools and kindergartens, together with the recommendation not to use grandparental care because they belong to a risky age group, has confronted many nurses and doctors with the question of whether to give priority to their patients or their families. Although special care institutions were opened for their children, their use presented practical complications. The crisis situation associated with the pandemic showed that family care commitments (for children or other family members) are perceived as private and therefore invisible, and are still primarily considered to be women's issues. Research shows that if the possibility of reconciling work and care in the health sector is not satisfactorily addressed, there is a risk of an exodus of these much-needed female workers into other fields.
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Meaning of virtual reality for a social group of NEETs
Bjaček, Jakub ; Pospíšilová, Marie (advisor) ; Krištofič, Peter (referee)
Jakub Bjaček Meaning of virtual reality for a social group of NEETs Abstract In this bachelor thesis I tried to look in more detail on the current issue of NEETs (acronym for "Not in Education, Employment, or Training"), in short, people who usually do not voluntarily participate in socialization through employment or institutional education. I was mainly interested in the reasons why this is so. Whether it is more about external causes (changes in the labour market, youth unemployment, competitive environment) or even causes of an internal nature. The question was where the NEETs are presenting themselves and how they spend time. That is why I focused on an online space in which a certain part of them could be active more visibly. This leaves me a relatively narrow target group of people who can be ranked in the NEETs category and also are active in the online space. These links have built research objectives. The question is how NEETs are presenting themselves in the online space. I was interested in virtual identity as a way to gain new social capital. This bachelor thesis is not about a comprehensive insight into the sociologically issue of NEETs as a whole, but about a certain view on this issue from the perspective of a particular activity (activities in the online environment and the perceptions and...
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Determination of rocuronium, vecuronium and pancuronium muscle relaxants by capillary electrophoresis with contactless conductivity detection
Böhmová, Lenka ; Polášek, Miroslav (advisor) ; Pospíšilová, Marie (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Student: Lenka Böhmová Supervisor: Doc. RNDr. Miroslav Polášek, CSc. Title of Diploma Thesis: Determination of rocuronium, vecuronium and pancuronium muscle relaxants by capillary electrophoresis with contactless conductivity detection A new method of capillary zone electrophoresis with contactless conductivity detection for the determination of pancuronium bromide, vecuronium bromide and rocuronium bromide was developed. The separation was carried out in fused-silica capillary with internal diameter 50 µm, total length 75 cm and effective length 45 cm. Optimal background electrolyte was 30mM acetate buffer of pH 5,75 containing 20 mg/ml of hydroxypropyl-γ-cyclodextrin. Phenyltrimethylammonium iodide was used as internal standard. The separation was performed at 25řC, the applied voltage was 20 kV. The samples were injected hydrodynamically at 50 mbar for 6 s. Under such optimal conditions the separation took less than 4 min. Calibration curves were linear for all analytes in the range 50 - 250 µg/ml, the coefficients of correlation were in the range 0,9954 - 0,9983. The limits of detection were 13,2 µg/ml, 11,1 µg/ml and 11,3 µg/ml for pancuronium bromide, vecuronium bromide and rocuronium...
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Analysis of cough-cold preparations by electrophoretic methods.
Kubíková, Drahomíra ; Pospíšilová, Marie (advisor) ; Jáč, Pavel (referee)
In this study, micellar elektrokinetic chromatography method (MEKC) was developed for the determination of paracetamol (PA), caffein (CO), ascorbic acid (AA) and phenylephrine (FE) in a pharmaceutical preparation. Separation was carried out in a fused silica capillary (31,2 cm x 50 m i.d. effective length 21 cm) at 20 kV with UV detection at 190 nm and 265 nm. Optimized background electrolyte (BGE) was 100 mM boric acid containing 100 mM sodium dodecyl sulfate (SDS) as a surfactant and 10 % (v/v) of methanol. pH* 7,8 was found to be optimal (modified by 50 mM sodium tetraborate). Methylparaben (MP) was used as internal standard. This validated method has been successfully applied for the analysis of commercially available pharmaceutical product (Coldrex tbl.).
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Development of capillary electromigration method for the determination of active compounds in silymarin I.
Riasová, Petra ; Jáč, Pavel (advisor) ; Pospíšilová, Marie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical chemistry Candidate: Petra Riasová Supervisor: PharmDr. Pavel Jáč, Ph.D. Title of diploma work: Development of capillary electromigration method for the determination of active compounds in silymarin I. This diploma thesis deals with development of the method to determine flavonolignans in silymarine using micellar electrokinetic chromatography. The method was optimized by examining a number of experimental conditions, such as concentration of boric acid, concentration of sodium dodecyl sulfate, concentration of cyklodextrins, volume fraction of organic modifier and applied voltage on separation. Ideal conditions for separation were not found by using univariate optimization, so it was applied chemometric approach for the development of separation method. The separation was carried out in a fused silica capillary (internal diameter 50 µm, total length 47,2 cm and effective length 38,7 cm), later in bubble cell capillary (internal diameter 25 µm, internal diameter of bubble cell 125 µm, total length 48,5 cm and effective length 40 cm), with UV detection at 200 and 320 nm. The capillary temperature was maintained at 25řC. Optimal conditions for separation of the flavonolignans were: 178 mM SDS, 15 mM...
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Using of Micellar Electrokinetic Chromatography in the Analysis of Pharmaceuticals
Lípová, Markéta ; Pospíšilová, Marie (advisor) ; Polášek, Miroslav (referee)
In this work, micellar electrokinetic chromatography (MEKC) with UV detection at 214 nm was developed for separation of paracetamol, caffeine, ascorbic acid and chlorphenamin. The validated method was used for selective assay of the active substances in pharmaceutical preparation. The separations were optimised by examining a number of experimental conditions, such as the electrolytic system type and concentration, pH, concentration of sodium dodecyl sulphate (SDS), addition of organic solvents, separation voltage and temperature. The optimal operational system consisted of 40mM boric acid, 70mM SDS and 10% (v/v) of methanol (adjusted to pH* 7.6 with 1 M ammonia solution). Ethylparaben was chosen as an internal standard. The assay of compounds analysed was carried out at 30 kV and 20ř C with 60 cm x 75 µm I.D. fused silica capillary (effective length 50 cm). A single analysis took less than 10 min. The method was suitable for determining the drugs in pharmaceutical preparation TRIGRIP capsules with RSD values from 1,23% to 2,75% (n=3). The MEKC results agreed well with nominal contents of the compounds. The recovery of the method ranged from 90.43% to 99.59%.
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Characterization of barbiturates and benzodiazepines-human serum protein interactions by cappilary electrophoresis
Studničková, Kamila ; Wsól, Vladimír (advisor) ; Pospíšilová, Marie (referee)
An important topic in the drug discovery and development process is the role of drug binding to plasma proteins. In this diploma thesis the characterization of the interaction between benzodiazepines and barbiturates towards human serum albumin and α-1-acid glycoprotein under physiological conditions by capillary electrophoresis-frontal analysis is presented. Furthermore, the binding of these drugs to all plasma proteins is evaluated by using ultrafiltration and capillary electrophoresis. The results indicate that the hydrophobic character of compounds seems to be the key factor on the interaction between these drugs towards proteins. In fact, hydrophobic basic drugs (benzodiazepines) bind in great extension to HSA, while less hydrophobic acid drugs (barbiturates) present lower interactions with proteins and bind especially to AGP.
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Analysis of natural compounds by capillary isotachophoresis and capillary zone electrophoresis.
Černá, Veronika ; Pospíšilová, Marie (advisor) ; Polášek, Miroslav (referee)
It has been designed tITP - CZE method with a reversed electro-osmotic flow for the analysis of seven organic acids of natural origin. It was monitorated the effect of boric buffer concentration, pH effect, the addition of organic solvent, the addition of cyclodextrin, concentration of NaCl as a stacker, and injection time. Measurements were made in voltage - 30 kV in capillary with inner diameter 50 μm and effective length 68 cm. Optimum separation conditions were found at a concentration of 125mM boric buffer, pH 8,2, the addition of 0,001% hexadimethrinbromid and addition of 10mM and 2,6-di-O- methylcyklodextrin. The stacker was used at a concentration of 200mM NaCl and injection time was 90 s. Intracapillary thermal gradient from 20o C to 24řC during electrophoresis has yielded improved resolution for the analytes.
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