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Activity of the Probation and Mediation Service in the Czech Republic
Plachká, Kateřina ; Šelleng, Dalibor (advisor) ; Mulák, Jiří (referee)
Activity of the Probation and Mediation Service in the Czech Republic Abstract and keywords The presented thesis deals with the position of the probation and mediation service (PMS) within the criminal law policy of the Czech Republic, its procedures in the field of probation, mediation and other actions it performs within the framework of the criminal process with adult offenders. This work covers juvenile offenders minimally. The aim is to broadly cover the entire activity and role of the probation and mediation service during criminal proceedings. For that reason, the work is divided into several basic sections. The first section deals with the question of punishment and different approaches to this issue, especially in terms of retributive versus restorative justice. A closer examination of the current use of these two approaches shows that we should not even set them against each other, but rather side by side. Furthermore, the work focuses on PMS as an institution. It deals with the way in which it was created, what are its main goals, the purpose for which it was established, but also how it is organized. The work also highlights some of the challenges and problems it currently faces, although overall we can declare its existence to be very beneficial. We discuss the tools through which it achieves...
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Optimization of the screening strategy for the detection of so-called new drugs of the synthetic cannabinoid group
Ozsvaldová, Silvia ; Šatínský, Dalibor (advisor) ; Plachká, Kateřina (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Silvia Ozsvaldová Supervisor: prof. RNDr. Dalibor Šatínský Ph.D. Consultant: PharmDr. Viktor Voříšek Titel of diploma thesis: Optimization of the screening strategy for the detection of so-called new drugs of the synthetic cannabinoid group Our diploma thesis pursues the main aim of optimizing the screening strategy of the capture of synthetic cannabinoids. In the theoretical part, we described the history of synthetic cannabinoids, their classification, and their effects on human health status. Next, we describe the determination, using the ultra-high liquid chromatography joined with mass spectrometry, which uses the principle of OrbitrapTM as a mass analyzer. We performed on our spiked sample of the JWH-018 N-(4-hydroxypentyl) on three different columns in the experimental part. There were Phenyl Hexyl Kinetex®, Luna Omega® Polar C18 and Arion® Polar C18 UHPLC columns. Also, we performed specific PRM scans on Luna and Arion columns. Samples on all three columns were measured in triplets, in duplicate during the PRM. We constructed calibration dependences, based on acquired values from which the parameters LOD and LOQ were calculated. Subsequently, we present these analytical control quality...
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The development of new methods of ultra-high performance supercritical fluid chromatography for pharmaceutical applications
Plachká, Kateřina ; Nováková, Lucie (advisor) ; Kučera, Radim (referee) ; Novák, Ondřej (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Mgr. Kateřina Plachká Supervisor: prof. Pharm.Dr. Lucie Nováková, Ph.D. Title of Doctoral Thesis: The development of new methods of ultra-high performance supercritical fluid chromatography for pharmaceutical applications A compilation of seven articles and three book chapters dealing with the applicability of supercritical fluid chromatography (SFC) methods in the field of pharmaceutical analysis is presented in this dissertation thesis. The first part focuses on pharmaceutical quality control and especially impurity control. The presented articles are dealing with the searching for generic screening approach for the development of SFC methods and with the subsequent optimization and validation of these methods. The screening conditions were compared based on developed mathematical model. The generic combination of diol stationary phase and 0.1 % ammonium hydroxide in methanol as modifier of CO2-based mobile phase was proposed as a starting point for SFC method development based on the obtained results. Several challenges were described during optimization of UHPSFC methods and their solution was proposed. The benefits of acetonitrile added to the modifier, advantages of column coupling and the...
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The development of new methods of ultra-high performance supercritical fluid chromatography for pharmaceutical applications
Plachká, Kateřina ; Nováková, Lucie (advisor) ; Kučera, Radim (referee) ; Novák, Ondřej (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Mgr. Kateřina Plachká Supervisor: prof. Pharm.Dr. Lucie Nováková, Ph.D. Title of Doctoral Thesis: The development of new methods of ultra-high performance supercritical fluid chromatography for pharmaceutical applications A compilation of seven articles and three book chapters dealing with the applicability of supercritical fluid chromatography (SFC) methods in the field of pharmaceutical analysis is presented in this dissertation thesis. The first part focuses on pharmaceutical quality control and especially impurity control. The presented articles are dealing with the searching for generic screening approach for the development of SFC methods and with the subsequent optimization and validation of these methods. The screening conditions were compared based on developed mathematical model. The generic combination of diol stationary phase and 0.1 % ammonium hydroxide in methanol as modifier of CO2-based mobile phase was proposed as a starting point for SFC method development based on the obtained results. Several challenges were described during optimization of UHPSFC methods and their solution was proposed. The benefits of acetonitrile added to the modifier, advantages of column coupling and the...
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Development and validation of method for the determination of agomelatin in tablets using UHPSFC and UHPLC
Plachká, Kateřina ; Nováková, Lucie (advisor) ; Khalikova, Maria (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Kateřina Plachká Supervisor: doc. PharmDr. Lucie Nováková, Ph.D. Title of Diploma Thesis: Development and validation of methods for the determination of agomelatine using UHPSFC and UHPLC Agomelatine is one of the newest antidepressants. Due to a different mechanism of action it offers a completely new approach in the treatment of depressive disorders. Two chromatographic methods for determination of agomelatine and its impurities were developed. The separations on UHPSFC system were accomplished using stationary phase based on BEH 2-EP and gradient elution using CO2 and MeOH containing 20mM ammonium formate and 5 % of water. The gradient was run from 5 - 30 % of organic modifier in 3 min. The UHPLC separations were performed on stationary phase BEH Shield RP18. The mixture of acetonitrile and methanol in ratio 1:1 and 10mM ammonium acetate buffer pH 9.5 were used as mobile phase. Both developed methods were properly validated in terms of linearity, sensitivity (LOD, LOQ), accuracy and precision according to ICH guidelines. The UHPSFC method was linear in the range 0.25-70 μg/ml for all analytes with method accuracy ≥ 97.4 % and ≥ 100.2 % and method precision RSD ≤ 2.4 and ≤ 0.8 for...
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