Original title: Optimalizace screeningové strategie záchytu tzv. nových drog skupiny syntetických kannabinoidů
Translated title: Optimization of the screening strategy for the detection of so-called new drugs of the synthetic cannabinoid group
Authors: Ozsvaldová, Silvia ; Šatínský, Dalibor (advisor) ; Plachká, Kateřina (referee)
Document type: Master’s theses
Year: 2021
Language: slo
Abstract: [eng] [cze]
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Silvia Ozsvaldová Supervisor: prof. RNDr. Dalibor Šatínský Ph.D. Consultant: PharmDr. Viktor Voříšek Titel of diploma thesis: Optimization of the screening strategy for the detection of so-called new drugs of the synthetic cannabinoid group Our diploma thesis pursues the main aim of optimizing the screening strategy of the capture of synthetic cannabinoids. In the theoretical part, we described the history of synthetic cannabinoids, their classification, and their effects on human health status. Next, we describe the determination, using the ultra-high liquid chromatography joined with mass spectrometry, which uses the principle of OrbitrapTM as a mass analyzer. We performed on our spiked sample of the JWH-018 N-(4-hydroxypentyl) on three different columns in the experimental part. There were Phenyl Hexyl Kinetex®, Luna Omega® Polar C18 and Arion® Polar C18 UHPLC columns. Also, we performed specific PRM scans on Luna and Arion columns. Samples on all three columns were measured in triplets, in duplicate during the PRM. We constructed calibration dependences, based on acquired values from which the parameters LOD and LOQ were calculated. Subsequently, we present these analytical control quality... Univerzita Karlova Farmaceutická fakulta v Hradci Králové Katedra analytickej chémie Autor: Bc. Silvia Ozsvaldová Vedúci práce: prof. RNDr. Dalibor Šatínský Ph.D. Konzultant: PharmDr. Viktor Voříšek Názov diplomovej práce: Optimalizácia screeningovej stratégie záchytu takzvaných nových drog skupiny syntetických kannabinoidov Naša diplomová práca sa zaoberá optimalizáciou screeningovej stratégie pre záchyt syntetických kannabinoidov. V teoretickej časti popisujeme históriu syntetických kannabinoidov, ich klasifikáciu a účinky na ľudský organizmus. Ďalej sa venujeme popisu stanovenia pomocou metódy vysoko kvapalinovej chromatografie s hmotnostnou spektrometriou, ktorá využíva ako hmotnostný analyzátor OrbitrapTM . V experimentálnej časti sme, ako vlastné meranie, v sére spikované vzorky JWH-018 N-(4-hydroxypentyl), vykonali na troch rôznych typov kolón. Išlo o kolóny Phenyl Hexyl Kinetex®, Luna Omega® Polar C18 a Arion® Polar C18 UHPLC. Pri kolónach Luna a Arion, boli vykonané ešte dodatočné merania prostredníctvom PRM skenu. Vzorky u všetkých troch kolón boli zmerané v tripletoch, pri PRM boli zmerané duplicitne. Na základe získaných hodnôt sme zostrojili kalibračné závislosti, z ktorých sa stanovili parametre LOD a LOQ. Následne boli tieto hodnoty porovnané a vyhodnotené. Kľúčové slová: syntetické...

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/126248

Permalink: http://www.nusl.cz/ntk/nusl-445835


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Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses