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Road bridge over the Kráľová waterworks
Pastucha, Marek ; Novák, Richard (referee) ; Nečas, Radim (advisor)
The subject of the thesis is the design of a road bridge over a river. A suspended bridge was chosen from three variants. The calculation of internal forces was done in the SCIA Engineer program and the assessment was done by manual calculation, in the MS Excel program and in the IDEA Statica program. The construction was assessed for the limit state of bearing capacity and usability. The calculation was made according to valid standards.standards.
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One span tee beam bridge
Pastucha, Marek ; Panáček, Josef (referee) ; Nečas, Radim (advisor)
The aim of this bachelor thesis is design of pedestrian bridge across the river, which is made from presstressed concrete. Out of three proposed variants was chosen beam construction which one support is fixed. Calculation of load cases was made by manual calculation and by softwares SCIA Engineer and MS Excel. The construction was designed and assessed to the ultimate limit state and the serviceability limit state. The calculation was made according to valid standards.
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One span tee beam bridge
Pastucha, Marek ; Panáček, Josef (referee) ; Nečas, Radim (advisor)
The aim of this bachelor thesis is design of pedestrian bridge across the river, which is made from presstressed concrete. Out of three proposed variants was chosen beam construction which one support is fixed. Calculation of load cases was made by manual calculation and by softwares SCIA Engineer and MS Excel. The construction was designed and assessed to the ultimate limit state and the serviceability limit state. The calculation was made according to valid standards.
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Photon-upconversion nanoparticles for single-molecule immunosensing of cancer biomarkers and bacteria
Farka, Z. ; Mickert, M. J. ; Hlaváček, Antonín ; Poláchová, V. ; Kostiv, Uliana ; Pastucha, M. ; Horák, Daniel ; Gorris, H. H. ; Skládal, P.
The recent progress in the field of immunoassays has been driven by introduction of various kinds of nanomaterials. In particular, photon-upconversion nanoparticles (UCNPs) proved to be excellent immunoassay labels due to their ability to emit light of shorter wavelengths under near-infrared excitation (anti-Stokes emission), which prevents autofluorescence, minimizes light scattering, and thus reduces the optical background interference. These unique photoluminescent properties allow counting of individual biomolecules labeled with UCNPs by conventional wide-field epiluminescence microscopy and enable the development of single-molecule (digital) immunoassays. We have introduced a novel label based on UCNPs conjugated with streptavidin via poly(ethylene glycol) and applied it in a digital upconversion-linked immunosorbent assay (ULISA) for the detection of a cancer biomarker prostate specific antigen (PSA). The digital readout based on counting of individual immunocomplexes improved the sensitivity 16× compared to conventional analog readout and allowed to reach a limit of detection (LOD) of 23 fg·mL−1 (800 aM). Human serum samples were successfully analyzed achieving an excellent correlation with electrochemiluminescence reference method. The conjugates of UCNPs with streptavidin are also suitable for the detection of pathogenic bacterium Melissococcus plutonius, the causative agent of honeybee disease European foulbrood. The ULISA assay provided an LOD of 340 CFU·mL−1 and successfully analyzed real samples of bees, larvae and bottom hive debris. Due to the high reliability and relatively simple detection scheme, the digital ULISA can pave the way for a new generation of digital immunoassays with a strong potential for commercialization.
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Schizophrenia, diabetes and 2nd generation antipsychotics
Pastucha, Miroslav ; Höschl, Cyril (advisor)
Choosing a theme for a thesis on 3.lékařské UK faculty facilitated by that individual clinics, institutes and departments already offer students the topics more or less established research programs. In addition, of course, there is still a possibility for the student himself devised a problem that will be paid. I was determined to choose topic that will be in the field of psychiatry, because this subject I find interesting and serious I am thinking that my focus after school might be based in this direction. Teaching psychiatry, which I attended in Prague Psychiatric Center, left me very positive impression of this device, both in terms of clinics and research work, and mainly from physicians access to patients as well as science. I was therefore glad that I had the opportunity to working on his thesis on the grounds of the institution. The association of two serious illnesses, schizophrenia and diabetes mellitus, was for me, up until I started to this thesis, virtually unknown. During internship in a psychiatric clinic, but I was surprised observed that many patients with schizophrenia are obese and they themselves admitted that the therapy of certain drugs feel increased appetite. I expected, therefore, that the pathogenesis of this problem will about moving in this direction, which is partly shown. I...
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