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Gel polymer electrolytes modified by ionic liquids with aprotic solvents
Pospíšilová, Michaela ; Sedlaříková, Marie (referee) ; Jahn, Michal (advisor)
This graduate thesis is focused on measuring the electrical conductivity of the gel polymeric electrolytes, their preparation and deals with the changes of the performance ratio of the individual substances in gel electrolyte The theoretical part deals with electrochemistry, gel electrolytes, the conductivity of electrolytes, gel electrolytes preparation, methods used to evaluate the measured results and on the properties of the ionic liquids. In the practical part is summarized the process of preparing gel electrolytes, the chemicals to prepare the gels with ionic liquids. The results are evaluated by using impedance technique and linear voltammetry, there are calculated the values of the electrical conductivity of the electrolyte and potential windows.
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The application of electrochemical impedance spectroscopy for investigation of enzyme kinetics
Pospíšilová, Michaela ; Sedláček, Jiří (referee) ; Fohlerová, Zdenka (advisor)
The application of electrochemical impedance spectroscopy (EIS) in theory and experiment for investigation of redox enzyme kinetics has been already described. The impedance studies are quite sensitive to the changes at the interface caused by adsorption of charged species and therefore can be used to study the kinetics of adsorption of macromolecules and also enables us to study enzyme kinetics since the action of the enzymes on their substrates involved modification and generation of charged species. In this work, we would explore the potential of electrochemical impedance technique to follow the kinetics of glucose oxidasesubstrate reactions on the immobilized surface. The enzyme will be allowed to interact with different concentrations of its substrate and the resulting reaction will be recorded in real time. Changes in the imaginary component of the impedance at various substrate concentrations will be expected to follow MichelisMenten kinetics. The experimental procedure should be simple and can be carried out directly in buffer solutions most suited for the required interaction to take place, without the requirement of any additional redox probes.
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Gel polymer electrolytes modified by ionic liquids with aprotic solvents
Pospíšilová, Michaela ; Sedlaříková, Marie (referee) ; Jahn, Michal (advisor)
This graduate thesis is focused on measuring the electrical conductivity of the gel polymeric electrolytes, their preparation and deals with the changes of the performance ratio of the individual substances in gel electrolyte The theoretical part deals with electrochemistry, gel electrolytes, the conductivity of electrolytes, gel electrolytes preparation, methods used to evaluate the measured results and on the properties of the ionic liquids. In the practical part is summarized the process of preparing gel electrolytes, the chemicals to prepare the gels with ionic liquids. The results are evaluated by using impedance technique and linear voltammetry, there are calculated the values of the electrical conductivity of the electrolyte and potential windows.
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Planning of evacuation, like a part of crisis preparedness Hospital of the Pardubice region, Ltd. - Hospital of Pardubice
POSPÍŠILOVÁ, Michaela
Abstract Purpose of this thesis was composet at two goals. Purpose of the first goal was evaluate present condition of Hospital Pardubice region, jsc. Hospital of Pardubice, personal's knowledges about behavior in cause of extraordinary event with emphasis on evacuation. Next goal was evaluate the overal status of preparedness of the Hospital Pardubice region, jsc. Hospital of Pardubice for evacuation. The basis of the theoretical part is in applicable legal standards of Czech republic, next this part describe planning of evacuation, including software planning, using modeling programs. In this part are describe events, when the evacuation has been done in real. The purpose of the practical part was to evaluate goals, which has been subsequently established by research questions and hypotheses. The purpose of answering the first research question, was evaluated the survey. The questionnaire has had 37 question and had been divided into 5 parts. The survey was realized during February and March 2018 at 6 departments of the Hospital Pardubice region, jsc. Hospital of Pardubice. The return of questionnaires was 58 %. More detailed specifications are given in chapter 4. To confirm whether the refutation of hypothesis nr. 1 was key respondents' answers to the questions asked, whose accuracy was compared based on the set 80% success rate of the right answers. In order to confirm whether or not the hypothesis nr. 2 was refuted, the results were subsequently processed by the 2 test statistic method - test of good agreement, which should confirm, or refute dependence of individual departments on their responses. For confirmation or refutation hypothese nr. 3, was compared results of this theses survey and results of bachelour thesis results. After the examination of the second research question, a comparison of the hospital's internal documentation with valid legal norms was carried out. An interview with the crisis manager was carried out, which examined the issue of population protection in the hospital. Last part of this research the analysis of questions (5, 7, 8, 13, 21, 24 and 25) of the questionnaire (Appendix 1). The overall success rate of staff awareness is 61 %, according to results. The overall preparedness of the hospital to evacuation is very good. The results of diploma thesis will be used especially for the analysis of own prepeardeness of the Hospital Pardubice region, jsc. Hospital of Pardubice for object evacuation.
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Comparing the depiction of Marshall's Plan by the press in contemporary Czechoslovakia
Pospíšilová, Michaela ; Cebe, Jan (advisor) ; Sekera, Martin (referee)
The bachelor's thesis focuses on how the Czechoslovakian press informed of preparations for Marshall Plan at the dawn of deciding whether or not to support this program. It compares the coverage of this topic by the Communist Party's Rudé Právo newspaper and the People's Party's Lidové Demokracie newspaper, the mainstream daily newspapers of the two parties in power, often having very different political views. The first two sections of the thesis elaborate on the topic from a historical point of view, tracking the post-war development in Czechoslovakia and throughout Europe, the growing rivalry between the United States and Soviet Union and events leading to Marshall Plan. The third section concentrates on the nature of the Czech media environment during the Third Republic and briefly depicts both examined periodicals. The fourth and fifth sections are devoted to the research and analysis of collected data.
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Planning of evacuation, like a part of crisis preparedness Hospital České Budějovice, a. s.
POSPÍŠILOVÁ, Michaela
The goal of bachelor thesis was to assess a current state of preparedness in Hospital České Budějovice, jsc for dealing with an extraordinary event. The stress was put on potential evacuation of the hospital. Next thing to assess was awareness of employees of Hospital České Budějovice, jsc about knowledge of recommended behaviour when an extraordinary event occurs. The emphasis was put on evacuation. The goal of the practical part was to answer formulated research questions. For assessment of first research question "How is the Hospital České Budějovice, jsc prepared for potential evacuation of the building?" and for answer of hypothesis no. 1 "Preparedness of Hospital České Budějovice, jsc for potential evacuation of the building meets the current requirements, following from applicable legislation", were chosen 3 criteria (chapter 2.2). For assessment of the second research question "How are the employees of Hospital České Budějovice, jsc informed about recommended behaviour during the extraordinary event with emphasis on evacuation?" and hypothesis no. 2 "Awareness of the employees in the Hospital České Budějovice, jcs about recommended behaviour during the extraordinary event with emphasis on evacuation come up to 80 % correct answers" and Hypothesis no. 3 "Awareness of the employees in the Hospital České Budějovice jcs, about recommended behaviour during the extraordinary event with emphasis on evacuation is not dependent on type of department" was chosen 1 criterion (chapter 2.2). The analysis of internal documents and the survey were done. These things were done for answer of research questions. The research using surveys was done among employees of the Hospital České Budějovice, jsc. The detailed description is stated in chapter 2.2. Questionnaire was consisted from 4 parts. The first part consisted of 6 question, which should characterize a respondent (workplace, sex, age, job categorization, length of practice, highest education level).Second and third part of questionnaire included 29 thematic questions, which deal with problems of protection of population, with evacuation and staff training. The questions of the fourth part of the questionnaire are for completion of these issues. These questions point out to self-evaluation of respondents and especially point out to opinions in these problems. Respondents answered for question, if they study in section of protection of the population alone, if they were acquainted with documents of medical facility, which is redestined for solution extraordinary event and if they consider this documents as sufficiently intelligible. Next respondents appraised own knowledge in part of protection of the population and attention, which is dedicated issues evacuation from side of Hospital České Budějovice, jsc. These question do not serving for answer to research questions, but for complex and unification of this issuess only. The results of the bachelor thesis will be used for preparation of employees in Hospital České Budějovice jsc for evacuation, including deepening their awareness about these problems.
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The application of electrochemical impedance spectroscopy for investigation of enzyme kinetics
Pospíšilová, Michaela ; Sedláček, Jiří (referee) ; Fohlerová, Zdenka (advisor)
The application of electrochemical impedance spectroscopy (EIS) in theory and experiment for investigation of redox enzyme kinetics has been already described. The impedance studies are quite sensitive to the changes at the interface caused by adsorption of charged species and therefore can be used to study the kinetics of adsorption of macromolecules and also enables us to study enzyme kinetics since the action of the enzymes on their substrates involved modification and generation of charged species. In this work, we would explore the potential of electrochemical impedance technique to follow the kinetics of glucose oxidasesubstrate reactions on the immobilized surface. The enzyme will be allowed to interact with different concentrations of its substrate and the resulting reaction will be recorded in real time. Changes in the imaginary component of the impedance at various substrate concentrations will be expected to follow MichelisMenten kinetics. The experimental procedure should be simple and can be carried out directly in buffer solutions most suited for the required interaction to take place, without the requirement of any additional redox probes.
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Diagnosis and therapy of Helicobacter pylori infection
POSPÍŠILOVÁ, Michaela
H pylori is a Gram-negative, microaerophilic rod-shaped moving bacterium, notorious for its urease production. It colonizes gastric mucosa and always leads to chronic gastritis, which may result in the development of other serious diseases, such as gastroduodenal ulcer disease and gastric adenocarcinoma. In 1994, H pylori was classified as a category 1 carcinogen. H pylori infection can be tested for invasively and noninvasively. The invasive methods include cultivation, histological examination and a rapid urease test. Noninvasive options are the carbon urea breath test, a stool antigen (HpSA) test and a blood antibody test. H pylori is sensitive to betalactam antibiotics. These should therefore be the essential part of the treatment; the instances of resistance are rare. Should the patient be allergic to betalactam antibiotics, nitroimidazoles can be used instead. H pylori is also sensitive to macrolides. The objective of my bachelor thesis was to acquire practical knowledge necessary for the correct processing of stool and serum samples in laboratory practice. I also intended to describe current trends in diagnosis and treatment of H pylori infection, together with the infection rates depending on age, sex and the season of the year. The findings were discussed in the context of relevant academic literature. Finally, I monitored the ways in which gastroduodenal ulcer disease caused by H pylori infection is diagnosed and treated by gastroenterologists in České Budějovice. The collection of the data for quantitative research was carried out between 1 January 2012 and 31 December 2012 in the microbiological laboratory Synlab czech s.r.o. in České Budějovice, which covers the whole of the South Bohemian Region. The samples were supplied mostly, but not exclusively, by GPs, and two methods of medical examination (euroSCREEN HP and EIA Helicobacter MONO IgG) were used. EuroSCREEN HP is based on the stool antigen (HpSA) detection, EIA Helicobacter MONO IgG is an immunoenzymatic method detecting IgG antibodies against H pylori in human serum or plasma. The data for qualitative research were collected in gastroenterologic ambulances in České Budějovice. Guided semi-structured interviews were carried out with one doctor in each ambulance. The acquired data were then processed as case studies and used to obtain categorization tables. The quantitative research confirmed all three hypotheses subject to evaluation. Higher age groups were found to have higher infections rates. However, no effect of either sex or the season of the year on the infection rates was registered. The qualitative research showed that gastroduodenal ulcer disease is most frequently diagnosed by České Budějovice gastroenterologists by the means of a biopsy check during gastroscopy. The samples are then sent to a microbiological laboratory for cultivation, histological examination and a rapid urease test. H pylori infection is usually treated by the standard triple therapy: amoxicillin, clarithromycin and a proton pump inhibitor. In case of hypersensitivity to betalactam antibiotics clarithromycin, a proton pump inhibitor and metronidazole are used instead. Occassional resistance to metronidazole and clarithromycin was reported. In these situations, cultivation is carried out in order to determine the sensitivity to antibiotics. The effects of the eradication treatment are evaluated by the means of a biopsy check during gastroscopy 6-8 weeks after its application. The samples are analysed in a microbiological laboratory. Some gastroenterologists also use a stool antigen (HpSA) test.
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