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Religious situation in Russia in the early 20th century
Nováková, Veronika Kateřina ; Šmíd, Marek (advisor) ; Čechura, Jaroslav (referee)
Master thesis Religious circumstances in Russia at the beginning of the 20th century will deal with religio-spiritual situation within czarist Russia since the dawn of 20th century till the rise of Bolshevik regime in 1917. It aims not only to analyse and describe religious circumstances of contemporary Orthodox church, but also reflect those circumstances in "minority" religious communities. It introduces the state at which the Orthodox church was at and enlighten the situation, that was influenced both by Russian course to industrial society and the outbreak of the First world war and revolution at 1917.
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Syntheses of azaphthalocyanine fluorescence sensors with the improved selectivity towards desired metal cations
Uhlířová, Štěpánka ; Nováková, Veronika (advisor) ; Zimčík, Petr (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department Department of Biophysics and Physical Chemistry Candidate Štěpánka Uhlířová Supervisor doc. PharmDr. Veronika Nováková, Ph.D. Title of thesis Syntheses of azaphthalocyanine fluorescence sensors with the improved selectivity towards desired metal cations Azaphthalocyanines (AzaPc) bearing aza-crowns on the periphery may be used as fluorescence sensors for metal cations. Emission in the red part of the spectrum is a great advantage of these compounds because such light is not absorbed by endogenous chromophores. Previous projects served mostly as a proof of concept where sensitivity of AzaPc sensors to a series of alkali and alkaline earth metal cations was shown. The aim of this diploma thesis is focused on the improvement the selectivity of a recognition moiety for particular cations. This can be achieved by the attachment of lariat ethers or crown ethers close to aza-crown moiety. Firstly, appropriate precursors, i.e. 5,6-disubstituted pyrazine-2,3-dicarbonitriles, were prepared by nucleophilic substitution. Then, cyclotetramerization of two different precursors (A and B) using a template method with zinc acetate in a high boiling solvent provided a statistical mixture of AzaPc congeners. Desired unsymmetrical AzaPc of ABBB type was...
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Synthesis of low-symmetry azaphthalocyanine for the labeling of DNA probes increasing the susceptibility of the molecular-biological methods
Schimmerová, Aneta ; Nováková, Veronika (advisor) ; Miletín, Miroslav (referee)
1. ABSTRACT Charles University, Faculty of Pharmacy in Hradec Králové Department Department od Biophysics and Physical Chemistry Student Aneta Schimmerová Supervisor Assoc. Prof. PharmDr. Veronika Nováková, Ph.D. Title of thesis Synthesis of low-symmetry azaphthalocyanine for the labeling of DNA probes increasing the susceptibility of the molecular- biological methods Azaphthalocyanines (AzaPc) are planar macrocyclic compounds. The alkylamino substituted derivatives can be used as dark quenchers in DNA-hybridization assays. A probe usually contains a quencher and a fluorophore. If these two moieties are close enough, the emitted fluorescence is quenched by the quencher. After the hydrolysis of the oligonucleotide probe, the fluorescence appears because of the long distance between the quencher and fluorophore. Thanks to the large system of conjugated double bounds, AzaPc absorb over a wide range of wavelengths from 300 nm to 750 nm. Such absorption covers all fluorophores used in hybridization assays nowadays, thus AzaPc may serve as universal dark quenchers. The aim of this study was to prepare an AzaPc, that will be possible to attach inside an oligonucleotide strand. Furthermore, a planar moiety bounded to AzaPc will intercalate into DNA and improve the strength of binding of the probe to DNA. First, the...
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Determination of fatty acids in human tissues
Turňová, Ivana ; Kuchařová, Monika (advisor) ; Nováková, Veronika (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Candidate: Ivana Turňová Supervisor of Diploma Thesis: Mgr. Monika Kuchařová, Ph.D. Consultant: prof. MUDr. Zdeněk Zadák, CSc. Title of Diploma Thesis: Determination of fatty acids in human tissues The fatty acids are non-negligible component of lipids as one of the basic nutrients. This thesis in its theoretical part presents above all the group of polyunsaturated fatty acids (PUFA), which are important structural units of the cell membranes, they are also the precursors to several significant biologically active substances. In the human organism PUFA participate in many physiological and pathological processes this way, where they cause the large spectrum of actions. Onwards the thesis describes the gas chromatography method that is used in chemical analysis of lipids for the fatty acid determination as the gold standard. Experimentally, the fatty acid representation was determined in the blood, muscle, heart, liver and kidney among 26 cadavers divided into two groups according to the presence/absence of inflammatory process at the time of death. The data obtained were statistically analysed. The results were descriptively evaluated and there were indicated possible explanations of differences...
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Socio-charitable works of the founders of the Pontifical Mission Societies
NOVÁKOVÁ, Veronika
The thesis deals with a probe into the past and the lives of the four founders of the Pontifical Mission Societies. It describes the value and purpose of the missionary activities. Furthemore, it introduces the missionary profession from the point of view of the church and of the three last Popes. It delivers an evidence of the life of the poor in missionary countries and a testimony of two missionaries. It shows both a positive and negative sides of the missionary activities. In the conclusion, it introduces projects supported by the Czech Republic.
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Czech private press agencies on exmaple of agency Herminapress
Nováková, Veronika ; Trunečková, Ludmila (advisor) ; Osvaldová, Barbora (referee)
Diploma thesis "Czech private press agencies on the example of Herminapress" presents work of the private press agency Herminapress which founded a journalist and photographer Jiří Herman originally as a photo agency in 2006, in the context of action private press agencies on a Czech media market after the year 1989 and in a present time. This private press agency is in the Czech media market unique, because as the only one it offers it's subscribers content in the form of a photograph, text and video, and it's clients also services which could be described as a public relations. This diploma thesis therefore puts a goal to define it's place on the Czech media market. By this work the author tries to define the difference between making the public servise content (ČTK) and work in private press company (Herminapress). The diploma thesis is based on the author's own three years lasting experience as an editor and photographer of the Herminapress, on the interviews with it's employees, clients and subscribers, and on the literature. The diploma thesis builds on a diploma and bachelor thesises which had been allready written to the issue of private press agencies on the czech media market after the year 1989. In the practical part of the work the author compares text and picture outputs of...
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The influence of anesthesia on the degree of DNA oxidative damage
Zubáňová, Veronika ; Kuchařová, Monika (advisor) ; Nováková, Veronika (referee)
Background: Oxidative damage is one of the most frequent types of cell components damage leading to oxidation of lipids, proteins and the molecule of DNA. As a consequence, there is a higher occurrence of several pathologies such as atherosclerosis, neurodegenerative diseases, cancer; or diabetes. In our study, influence of whole body anesthesia during minor surgery on the level of DNA damage was examined using comet assay technique. Methods: The basic principle of this method is fixing the cells (lymphocytes) in agarose, their lysis for the removal of membranes, incubation with the specific enzymes and electrophoresis of the released cell nuclei. During the electrophoresis, free low-molecular weight and negatively charged fragments of DNA move towards anode which causes the formation of the typical comet cell shape. Finally, the gels are stained by ethidium bromide (DNA intercalating dye) and visualized. Results: We have observed single strand breakages (SSBs) and, with the use of modified assay using specific enzymes for detection of specific lesions, also oxidized purines and pyrimidines. The extent of DNA damage as determined by the intensity of the tail of the comet was quantified using LUCIA Comet Assay (Laboratory Imaging, Czech Republic) software for image analysis. The results were used...
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Miniaturized and fast method for solubility and level of supersaturation determinations of drug nanocrystals
Paľová, Romana ; Nováková, Veronika (advisor) ; Kuchařová, Monika (referee)
Title of thesis: Miniaturized and fast method for solubility and level of supersaturation determinations of drug nanocrystals Author: Romana Paľová Department: Biophysics and Physical Chemistry Supervisor: Doc. Veronika Nováková, Ph.D. Specialized supervisor: Assoc. Prof. Leena Peltonen, Ph.D. As a formulation strategy for improving the bioavailability of poorly water-soluble drugs, drug nanocrystals can be employed. Several studies have shown that drug nanocrystals exhibit higher dissolution rate compared to conventional drug formulations. However, when orally administered, various conditions in gastrointestinal tract, such as changes in pH may result in problematic absorption. In this work, the preparation of drug nanosuspension of itraconazole (ITZ), as model poorly water-soluble compound, was carried out. The nanosuspension was prepared by using wet milling method of preparation, with suitable stabilizers added (Poloxamer 407 and Hydroxypropyl methylcellulose). Hydrochloric acid buffer pH 1.2 was chosen to create an environment similar to gastric fluids. In order to investigate changes in solubility of ITZ nanosuspension, UV spectrophotometer was used. Measurement was performed at the wavelength 551 nm, where dissolved ITZ does not absorb light and no change in absorbance occurred....
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The impaired change in plasma long-chain acylcarnitine level as a marker of insulin resistence
Šišmová, Petra ; Nováková, Veronika (advisor) ; Čečková, Martina (referee)
Charles University Faculty of Pharmacy in Hradec Kralove Department of Biophysics and Physical Chemistry Rīga Stradiņš University Latvian Institute of Organic Synthesis Laboratory of Pharmaceutical Pharmacology Candidate: Petra Šišmová Supervisor: Dr. Pharm. Elina Makarova, assoc. prof. Veronika Nováková, Ph.D. Title of the diploma thesis: The impaired change in plasma long-chain acylcarnitine level as a marker of insulin resistance Insulin resistance presents one of the factors that could lead to type 2 diabetes mellitus (T2DM). Increased levels of long-chain (LC)-acylcarnitines are associated with obesity and T2DM and recent studies suggest that LC-acylcarnitines could play a role in the development of insulin resistance. Early diagnosis of insulin resistance would help to prevent the onset of T2DM and the development of associated complications. The purpose of this study was to determine whether the measurements of the changes in acylcarnitine concentrations during the glucose tolerance test (GTT) could be used as a novel marker of insulin resistance. After intraperitoneal administration of glucose in high fat diet (HFD)-fed C57bl/6N, db/db mice and respective controls the concentrations of glucose, insulin, glycated haemoglobin, free fatty acids (FFAs) and acylcarnitine levels in the fasted...
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