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The role of UDP-glycosyltransferases in herbicide resistance
Mudrová, Dagmar ; Podlipná, Radka (advisor) ; Hála, Michal (referee)
Along with the widespread use of herbicides in modern agriculture, the phenomenon of herbicide resistance has appeared, where weed plants become partially or completely resistant to the action of one or more herbicides. Resistance arises in two ways - by a change in the target molecule on which the herbicide acts, so-called "target site resistance" (TSR), or by a change in other non-target proteins, so-called "non-target site resistance" (NTSR). UDPglycosyltransferases (UGT), which glycosylate a wide range of endogenous plant molecules and xenobiotics, are also involved in NTSR. Also, increased activity of antioxidant enzymes may contribute to NTSR. This work was focused on the interaction of the herbicides pinoxaden and sulcotrione with the model plant Arabidopsis thaliana. The aim of this work was to determine whether some of the selected UGT genes change their expression profile after contact with pinoxaden, and whether these changes are influenced by the contact of plants in previous generations with pinoxaden. The expression of selected UGT genes was also monitored in plants treated with sulcotrione. It was also monitored whether the activity of selected antioxidant enzymes changes in plants that were treated with the herbicide pinoxaden or sulcotrione. The results showed that in the case of...
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