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Diversity and distribution of the Euastrum humerosum / didelta (Desmidiales) species complex
Kupčíková, Eva ; Šťastný, Jan (advisor) ; Kollár, Jan (referee)
From 23 European and North American strains Euastrum humerosum/didelta species complex (Desmidiales) I have obtained 3 lineages in molecular marker trnGucc group II intron. I did not acquire significant and homogenous results in SSU intron. It is probable that the lineages of trnGucc intron are very young. SEM revealed one central pore in cell wall and five bulges. The lineages were significantly different in their shape and dimensions. The lineages differentiated mainly in their length and breadth of the cells. The lineages inside morphotype E. humerosum differentiated in shape and dimensions of the polar lobe. The lineages inside morphotype E.didelta differentiated in shape and dimensions of the neck. That was ascertained using the geometric morphometrics with the measuring. Linear discriminant analysis of the desmids from literature revealed that is possible to partly discriminate groups of the varieties (E. humerosum var. parallelum and E. didelta in lineage A; two formae E. didelta f. val Piora and E. didelta f. latior in lineage B; E. didelta var. inermiforme and E. humerosum var. affine in lineage C). Key words: desmids, species, hidden diversity, molecular phylogenetics, geometric morphometrics, Euastrum, scanning electron microscopy
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Diversity and distribution of the Euastrum humerosum / didelta (Desmidiales) species complex
Kupčíková, Eva ; Šťastný, Jan (advisor) ; Kollár, Jan (referee)
From 23 European and North American strains Euastrum humerosum/didelta species complex (Desmidiales) we have obtained 3 lineages in molecular marker trnGuuc group II intron. We did not acquire significant and homogenous results in SSU intron. It is probable that the lineages are very young. The lineages were significantly different in their shape and dimensions. We used the geometric morphometrics with the measuring of the lengths and breath of the semicells, isthmus and their ratios. The lineages differentiated in their dimensions and shape variable polar lobes and neck. SEM revealed the one central pore in cell wall on the contrary, with variant E. didelta var. bengalicum, which has two pores according the literature records. Key words: desmids, species, hidden diversity, molecular phylogenetics, geometric morphometrics, Euastrum, scanning electron microscopy
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Cryptic and pseudocryptic diversity of conjugating green algae (Zygnematophyceae)
Kupčíková, Eva ; Šťastný, Jan (advisor) ; Škaloud, Pavel (referee)
This bachelor thesis summarizes the most commonly used species concepts in the class Zygnematophyceae. This group of algae and the molecular markers used in the zygnematophytes' molecular phylogeny will be presented in this thesis. Zygnematophytes have unicellular or multicellular forms and they generally occur in freshwater habitats. Desmids are useful as indicators of water quality and they are also used for scoring of conservation value. Therefore it is important to have the species well defined. The confusion in defining of desmid species was caused by often very variable morphology. Some authors had different opinions on the classifying of desmid species and theirs subunits. The combination of molecular phylogeny, electron microscopy and geometric morphometrics was used in revealing of (pseudo)cryptic species in the genera Micrasterias or Xanthidium. The geometic morphometrics was helpful for finding of important details for distinguishing of particular pseudocryptic species. Key words: cryptic and pseudocryptic diversity, conjugation, desmids, Micrasterias, Euastrum, Xanthidium, molecular phylogeny, taxonomy, geometric morphometrics, biogeography, green algae
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Corporate Governance
Kupčiková, Eva ; Pirožek, Petr (advisor) ; Král, Pavel (referee)
The diploma thesis deals with issues of administration and control of a company, so-called corporate governance. It deals with relationships and links between the owners and management that need to be regulated because the ownership is separated from the management and control. The aim of this work is a thorough examination of the current state of corporate governance at a particular company. The primary object of the examination is the ownership and organizational structure, individual elements, their relationships and competencies and subsequent evaluation of the effectiveness and efficiency of the applied system.
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