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The use of JavaScript MVC frameworks for creating web applications
KOLÁŘ, David
The objective of the bachelor thesis will be develop and describe issues related to JavaScritp MVC frameworks. How to simplify and streamline the work of the code when creating web applications in terms of client-side scripting. I will focus to compare the busiest frameworks, their advantages, disadvantages, when and why it pays to use. I will include in my selection AngularJS, React, Knockout and mention the other frameworks. The practical part will consist of examples particular frameworks while solving real problems. For this purpose, I will create a Web application, which will include an overview of their properties, and comparing the aforementioned examples.
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Criminal Liability within Pharmacy
Kolář, David ; Sovová, Olga (advisor) ; Císařová, Dagmar (referee)
The main purpose of this thesis is to analyse the possible application of criminal liability instruments within pharmacy. Chapter 1 introduces the general aspects and processes within pharmacy, general terms, and short preview to the life cycle of innovative medicinal products. Chapter 2 contains the general definition and requirements of liability. There is also a short comparison of additional types of liability (such as civil, employee's, administrative and disciplinary) that can be applicable within pharmacy. The purpose of this comparison is to specify differences between individual liabilities and to prove that they can be applied conjointly. As an example of such a use I have described the "heparin murder" case. Chapter 3 is dedicated to criminal liability, with the explanation of general terms, especially crime, unlawfulness, offender, or grounds of justification. Chapter 4 focuses on the criminal liability of natural persons. After the general introduction in the first part, a list of potential crimes committable within pharmacy is given. Chapter 5 summarizes the criminal liability of legal persons. The first part focuses on the history of implementation in the Czech Republic, general terms, and definition of a legal person as a subject of criminal liability. The second part contains...
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Trafficking of purinergic P2X receptor in eukaryotic cell
Pražák, Šimon ; Tvrdoňová, Vendula (advisor) ; Kolář, David (referee)
Purinergic receptors are membrane ion channels activated by extracellular ATP. In vertebrates, seven genes encoding P2X subunits was found. These subunits are designated as P2X1 - 7. Every P2X receptor subunit consists of two transmembrane domains, extracellular domain and intracellular N- and C- termini. P2X receptors fold to homo- or heterotrimers. P2X receptors have a wide distribution in the organism, functional receptors are found in neurons, glial cells, muscle cells and also in nonexcitable tissues as epithelial, endothelial, and in hemopoietic tissue. Purinergic signalling plays an important role in pain transmission, CNS injury and immune processes. P2X receptors are synthesized on the rough endoplasmic reticulum and are transported to the plasma membrane after post-translational modifications in the Golgi apparatus. The distribution and transport of P2X receptors is subunit specific and dependent on the cell type in which they are expressed. P2X receptors can be divided into three groups according to the way they are moved in the cell, which differ in transport speed, plasma membrane accumulation rate and rate of internalization.
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The role of microRNA in hypoxic pulmonary hypertension
Tichý, Václav ; Hampl, Václav (advisor) ; Kolář, David (referee)
Pulmonary Arterial hypertension (PAH) is a devastating progressive disease that significantly decreases quality of life and has the average survival rate of only few years. One of the significant initiators of PAH is chronic hypoxia. After more than six decades of research that was initiated in 1946 by von Euler and Liljestrand, a new group of potential regulators of this pathology was discovered, that became heavily studied in the last five years. They are highly conserved molecules belonging to non-coding RNA. These 19-23 nucleotides long microRNA (miRNA) act as negative regulators of expression on various proteins. Many of them regulate traditional signalling pathways of hypoxic PAH (HIF-1, BMPR2) and miRNA is in turn regulated by other signalizations. Together, that creates an interconnected network of direct and indirect interactions and feedback loops, that we need to study in order to understand hypoxic PAH. This thesis summarizes findings about important miRNA molecules from the last few years and elucidates part of these regulatory mechanisms on several miRNA molecules (miR-17-92, miR-21, miR-210, miR-204 a miR143/145).
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Beta-adrenergic receptors and their desensitization
Biriczová, Lilla ; Novotný, Jiří (advisor) ; Kolář, David (referee)
β-Adrenergic receptors (β-ARs) are G-protein-coupled receptors (GPCR), widely present in the animal organism and mediate catecholamine pathways leading to diverse physiological responses. The family of β-ARs consists of β1-AR, β2-AR and β3-AR, which are distinguished by their affinity to adrenaline and noradrenaline. A typical model of β-AR signalling includes binding of the ligand, G-protein coupling, activation of adenylyl cyclase (AC) resulting in production of the second messenger cAMP and activation of protein kinase A (PKA) that phosphorylates downstream proteins leading to physiological responses. Beacause excessive catecholamine signalling can cause undesirable consequences, a mechanism has evolved, which attenuates the function of β-ARs in spite of further stimulation, so called desensitization. The classic course of desensitization consists of characteristic steps including phosphorylation of the receptor, β-arrestin attachement and uncoupling of the G-protein from β-AR. Restoration of the signalling ability is allowed through resensitization of β-AR when the receptor is sequestrated and dephosphorylated. Given that β-ARs are structurally and genetically different, it is reasonable to consider that each step of the desenstization process may happen differently among the different subtypes...
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Brain energy metabolism and its pathologies
Nová, Jana ; Telenský, Petr (advisor) ; Kolář, David (referee)
The brain belongs amongst metabolically most active organs and disturbances of brain energy metabolism are commonly cited as a critical factor predisposing to neurodegeneration. Brain metabolic substrates must be strictly regulated both spatially and temporarily to ensure the energy homeostasis of the brain. Research on energy metabolism of the brain identified several models revealing the interdependence of metabolic processes in the CNS. Powered by TCPDF (www.tcpdf.org)
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Calcium signalling in astrocytes under physiological and pathological conditions
Svatoňová, Petra ; Anděrová, Miroslava (advisor) ; Kolář, David (referee)
Calcium signalling in astrocytes represents an important component, which enables proper neuronal functioning under physiological conditions. Alterations in Ca2+ signalling, accompanied by an increase in intracellular calcium levels is a hallmark for numerous pathological states of central nervous system, such as traumatic and ischemic brain/spinal cord injuries, epilepsy as well as neurodegenerative diseases, such as Alzheimer's disease and psychiatric disorders, such as schizophrenia. The research analyzing the molecular components of astrocytic Ca2+ signalling can help us understand the control mechanisms used in calcium signalling and thus be greatly beneficial for further therapeutic research. Powered by TCPDF (www.tcpdf.org)
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Cardioprotective role of epoxyeicosatrienoic acids in ischemia-reperfusion injury.
Veselá, Barbora ; Neckář, Jan (advisor) ; Kolář, David (referee)
Epoxyeicosatrienoic acids (EETs) are arachidonic acids metabolites that importantly contribute to vascular and cardiac physiology and pathophysiology. Current research in the field of EETs shows that these molecules can significantly reduce the rate of acute ischemia- reperfusion injury of the heart. However, they can also contribute significantly to the recovery of the heart muscle following myocardial infarction and to reduce the development of post- ischemic heart failure. The project aims to outline the known cardioprotective effect(-s) of EETs on myocardial ischemia/reperfusion injury and their mechanisms. Key words: heart, ischemia-reperfusion, epoxyeicosatrienoic acids
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The role of PGC1 in cardioprotective mechanisms
Jeřábková, Adéla ; Horníková, Daniela (advisor) ; Kolář, David (referee)
One of the leading causes of death worldwide is cardiovascular diseases. Researchers are, therefore, dealing with the mechanisms that induce a cardioprotection. Cardioprotection is a general pathophysiological term under which we understand myocytes protection against damage by ischemia and subsequent reperfusion impairment, inflammation, hypertension, and toxic and degenerative changes, including some types of apoptosis. One of the less common ways of cardioprotection is a cold adaptation. Adaptive thermogenesis is an important part of energy homeostasis and protection against obesity, metabolic disorder threatening heart. The PGC family of proteins plays a very important role in adaptive thermogenesis. This thesis summarizes the current state of literature in cold adaptation issues, especially the role of PGC1α and its effects at the cellular and tissue level. mRNA expression of PGC-1α is strongly induced in brown fat and skeletal muscles of mice exposed to cold. PGC-1α also increases the transcriptional activity of PPAR-γ and thyroid hormone receptor protein on UCP-1 (uncoupling protein). UCPs (uncoupling proteins) are small proteins localized to the inner side of the mitochondrial membrane to facilitate the transport of protons, which they release into concentration gradient without ATP...
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