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Changes in nutritional status during high-dose chemotherapy in selected hematooncological diseases
Křivánková, Jana ; Szotkowski, Tomáš (advisor) ; Káňová, Marcela (referee)
Hematooncological diseases are often accompanied by dietary restriction, especially in cytotoxic therapy. The main purpose of the work was to assess the effect of high-dose chemotherapy on the change of nutritional status in two groups of hematooncological patients. A total of 16 patients were enrolled. Changes of the body composition were evaluated using bioelectrical impedance analysis supplemented by monitoring of biochemical nutritional indicators. Observations showed that in both groups the majority lost weight. In the first group of eight patients with acute myeloid leukemia observed during three consecutive hospitalizations, the median of change of body weight was -3.7 kg (-4.3%). Loss of lean body mass with a median value of -4.8 kg (-7.2%) was detected at all patients. Body fat was reduced at half of the patients. In some cases, with length of observation, there was an increase in fat mass along with visceral fat. In the second group, which included eight patients (after autologous hematopoietic stem cell transplantation) at whom one hospitalization was evaluated, body weight was reduced at six patients. The medianof change of body weight was -2.1 kg (-2.3%). At five patients, the treatment representeda loss of active metabolic mass. The change of the weight of the lean body mass was shown...
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Photon-upconversion scanner for multiplexed imaging
Hlaváček, Antonín ; Křivánková, Jana ; Foret, František
In analytical chemistry, multiplexed assays facilitate parallel detection of analytes. The multiplexing reduces the consumption of sample, reagents, and other resources and can be applied for medical diagnostics, the studies of biomolecule interactions, single-cell and singlemolecule assays, environmental monitoring, pathogen detection, multiparameter chemical and biological assays and screening of chemical libraries.
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Fast and highly sensitive laser scanner for recording photon-upconversion luminiscence from planar surfaces
Hlaváček, Antonín ; Křivánková, Jana ; Foret, František
Photon-upconversion nanoparticles (UCNPs) are lanthanide-doped nanocrystals that can be excited by nearinfrared light and emit photon-upconversion luminescence of shorter wavelengths. Advantages of UCNPs include near-infrared excitation, multiple and narrow emission bands, negligible autofluorescence and high stability, which make UCNPs ideal luminescence label for use in biological and chemical assays. These assays - e.g. upconversion-linked immunosorbent assay, western blot, lateral flow assay, gel electrophoresis, thin layer chromatography - commonly require the scanning of a planar surface with a high spatial resolution and an excellent sensitivity. The availability of commercial equipment is recently limited because of the novelty of the photon-upconversion phenomenon. Therefore, we report on the construction of photon-upconversion laser scanner. The scanner consists of a laser scanning head, which is attached to a xy-moving stage. The scanning head itself is constructed as an epiluminescence detector with excitation wavelength of 976 nm. A CCD array spectroscope is connected to the laser head and serves as a sensitive detector of photon-upconversion luminescence. The scanner possesses a spatial resolution of 200 μm, the scanning rate is up to 57 points per second and the sensitivity reaches down to single photon-upconversion nanoparticle.
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Mistakes in Writing from the Psycholinguistic Perspective
Křivánková, Jana ; Chromý, Jan (advisor) ; Adam, Robert (referee)
The thesis is concerned with speech errors and errors in writing. It summarizes the theories of spoken and written language production and specifies basic types of speech errors and errors in writing. The interpretation of typing errors is presented which is based on a corpus of errors collected directly for this purpose. The corpus also serves as a basis for statistical analysis of the frequency of typing errors. The thesis also compares types of speech errors and errors in writing and identifies main differences and similarities between them.
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Development of microfluidic device for droplet generation and microparticle encapsulation
Křivánková, Jana ; Foret, František
Microfluidic devices combined with various bioanalytical and optical sensors proved\ntheir potential in chemical, biological, and biomedicine applications. At a droplet\nmicrofluidic device a fluid is divided into numerous droplets surrounded by a\ncontinuous immiscible fluid phase. Generated droplets serve then as\nvessels/microcapsules for delivering drugs, nutrients as well as for encapsulating of\nbiologically active particles and cells.\nDescribed experimental study deals with the development of a practical dropletbased\nmicrofluidic chip useful for production of monodisperse water/oil emulsion and\nfor encapsulation of thin-metal magnetic microsheets.
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Simple flow-focusing microfluidic chip for droplet generation
Křivánková, Jana ; Basova, E. Y. ; Foret, František
There is an ascending requirement for analysis of chemical content of individual cells involving combination of various bioanalytical and optical sensors and arrangements. At the beginning of the cell analysis there is a need for creation of suitable transport vessels - droplets - that can be easily counted, sorted and serve for manipulation of cells with preserved biocompability as well. Among all of bioanalytical approaches droplet-based microfluidics proved its potential for producing droplets with precisely controlled size, shape as well as for encapsulation of reactants and delivering drugs. This study presents a simple microfluidic chip with flow-focusing geometry for generation of water droplets in fluorocarbon oil.
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An accounting representation of foreign currency operations in the tourist industry area
Křivánková, Jana ; Procházka, David (advisor) ; Roubíčková, Jaroslava (referee)
The main goal of this bachelors thesis is to represent foreign currency operations and rate differences according to the Czech legislation in contrast with International Financial Reporting Standards IFRS/IAS while directing at the tourist industry area. The first part of this work covers the introduction into the tourist industry area and its history in the Czech Republic including factual information. The introducing part is followed by theoretical definition of the Czech legislation in comparison with International Financial Reporting Standards IFRS/IAS, both focusing on foreign currency. Several particular examples are represented subsequently in each chapter. The conclusion of this thesis is dedicated to the comparison between both aforementioned systems and different possible consequences in final accounts.
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