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Bioactive molecules involved in blood processing by haematophagous monogeneans of the family Diplozoidae
Jedličková, Lucie ; Mikeš, Libor (advisor) ; Horn, Martin (referee) ; Sojka, Daniel (referee)
Monogeneans from the family Diplozoidae (subclass Heteronchoinea) are bloodfeeding ectoparasites inhabiting gills of common carp. Digestion of blood in diplozoids is an intracellular process taking place in gut cells within lysosomal cycle in the presence of parasite's peptidases. However, information about the blood digestion comes only from ultrastructural and histochemical analyses. Therefore, I have focused in this work on biochemical and molecular characteristics of bioactive molecules which may participate in blood processing by E. nipponicum adults, especially cysteine peptidases of cathepsin L- and B- types, aspartic peptidases of cathepsin D-type, and Kunitz-type inhibitors of serine peptidases. In homogenates and excretory/secretory (E/S) products of E. nipponicum adults, an activity of cysteine peptidases of cathepsins L-type dominated, followed by an activity of cathepsin D-like aspartic peptidases and a minor cathepsin B-like activity. Inhibitors of the abovementioned peptidase types completely blocked hemoglobinolytic activity in the samples. In the transcriptome of E. nipponicum adults, ten cathepsin L-coding transcripts were found and only one cathepsin B-coding transcript. Primary structures of the encoded enzymes were bioinformatically and phylogenetically compared. Two abundant...
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Peptidases of monogeneans of the family Diplozoidae
Jedličková, Lucie ; Mikeš, Libor (advisor) ; Dvořák, Jan (referee)
The blood processing mechanisms in monogeneans of the subclass Polyopisthocotylea are known from ultrastructural and histochemical analyses only. In contrast to other blood- feeding parasites, just few biochemical and molecular analyses have been done on digestive enzymes in monogeneans. Therefore, we focused on the biochemical and molecular characterization of hydrolytic enzymes (peptidases) in the hematophagous species Paradiplozoon bliccae and Eudiplozoon nipponicum. The presence of the cysteine class peptidases, mainly cathepsin L, in excretory- secretory products and soluble protein extracts of P. bliccae and E. nipponicum we found. Detection was carried out using fluorogenic substrates, specific inhibitors and the labelled probe DCG-04. On the gels / membranes after electrophoresis / blotting we detected bands of approximately size of 35 kDa in the case of both species and 24 kDa for E. nipponicum. Soluble protein extracts of worms were separated by 2D gel electrophoresis and relevant spots around 35 kDa (P. bliccae) and around 25 ˗ 35 kDa (E. nipponicum) were confirmed by mass spectrometry as cathepsins L. Using degenerate primers based on the conserved motifs of cysteine class peptidases, a partial sequence of cathepsin L gene from E. nipponicum was obtained. Furthermore, 3'RACE PCR method...
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Possibilities of prevention and treatment of eating disorders
Jedličková, Lucie ; Schneidrová, Dagmar (advisor)
Eating disorders are a serious chronic illness, their incidence is increasing, especially among young girls and women. Therefore it is very important to inform the public about issues associated with eating disorders and experts in the quest for education and prevention programs, especially for the highest risk population, ie young girls. In my work, I present an overview of eating disorders, their etiology, incidence and in particular the possibility of current therapy and prevention.
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Marketing Utilization in Service Sector
Jedličková, Lucie ; Čermák, Lubomír (referee) ; Chalupský, Vladimír (advisor)
This diploma work deals with marketing utilization in service sektor, concrete in legal profession. The objective was to make analysis of marketing assertion in legal office. On the basis of suitable methods was followed and evaluated actual posture of marketing literacy in legal office. Result of practical analyses was definition of barriers, handicaps, reserves and opportunities at marketing utilization in legal office. Theoretical part features information about marketing in service sektor, shows in legal profession domain and gets acquainted with used methods. Practical part analyses contemporary state in legal office. By means of suitable methods is drafted the measure, how to eliminate barriers, handicaps and reserves and to take advantage of opportunities.
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Evaluation of company information needs suporting market orientation
Jedličková, Lucie ; Hrabal, Vladimír (referee) ; Šimberová, Iveta (advisor)
This work deals with evaluation of company information needs suporting market orientation. The objective was to make analyzis of current company posture, draft possibilities of improvement and make such company facilities with informations, which could lead to better and effective market operations. Theoretical part features information about informative science, communications, informative management and knowledge management. Practical part analyzes departmental environment and also the company itself. Summing up current position the measure, how to improve informationsflow and communicationsflow needs suporting market orientation, is draft.
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Effectiveness of Marketing Activities in Preparatory Phase of Construction Life Cycle
Jedličková, Lucie ; Simová, Josefína (referee) ; Stávková, Jana (referee) ; Zúbková, Mária (referee) ; Šimberová, Iveta (advisor)
This doctoral thesis titled Effectiveness of marketing activities in preparatory phase of construction life cycle deals with the possibility of more effective marketing activities in preparation for construction. The main goal of the thesis is to analyze the current state of the use of marketing activities from the view of a building contractor and building users, to propose a methodology of indicators suitable for assessing the effectiveness of marketing activities in preparatory phase of construction life cycle. Effectiveness is assessed from the perspective of selected entities operating in the Czech construction market - building contractor and building user.
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Technical Analysis
Krčová, Vendula ; Jedličková, Lucie (referee) ; Novotná, Veronika (advisor)
This diploma thesis is focused on the general characteristics of the technical analysis, definitions of the information sources and technical analyses. Main attention is given to the particular indicators of the technical analysis. Practical part of this thesis includes applying of the defined indicators in the evaluation of the money market situation.
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