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3D printed opto-mechanical devices
Šremrová, Vendula ; Dostál, Zbyněk (referee) ; Jákl, Petr (advisor)
Optomechanical components are widely used in many optical experiments. This diploma thesis deals with design and manufacturing optomechanical components using 3D print technology. These are cheaper alternatives of commercial devices. In addition to 3D printed parts, minimum number of other components are used to assemble functional devices. Using simple experimental setups, the manufactured components are evaluated and compared with commercially available ones. The results show that they can be used in applications where high accuracy is not required. The second part is devoted to the design and manufacturing of a polarimeter as a mechanism combining electrical and mechanical components with 3D printed parts. The polarimeter is used to measure some properties of polarized light.
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Preparation and analysis of fibre probes for holographic microendoscopy
Černá, Eliška ; Průša, Stanislav (referee) ; Jákl, Petr (advisor)
This theses deals with the preparation and analysis of fibre probes for a holographic microendoscope. The fibres are specifically sanded and a reflective layer of aluminium or silver is applied on them by thin film deposition. The properties of these deposited layers are further examined and evaluated using AFM, profilometer and measuring apparatus to assess the reflectivity. The protocol describing the production of fibre-optic probes with optimal parameters for holographic microendoscopy is created from measured data. Such fibre probes could be beneficial in the diagnosis of neurodegenerative diseases such as Alzheimer’s or Parkinson’s disease.
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Multimode optical fiber based endoscopy
Jákl, Petr ; Tučková, Tereza ; Pikálek, Tomáš ; Stibůrek, Miroslav ; Ondráčková, Petra ; Cifuentes, Angel S. ; Šiler, Martin ; Uhlířová, Hana ; Traegaardh, Johanna ; Čižmár, Tomáš
Optical microscopy is a technique for microworld investigation using light waves scattered on particles in sample space. Its main disadvantage in the area of medicine, microbiology and neurology is its low penetration depth - it is very difficult to image structures deeper than approximately 1 mm inside tissue. Conventional endoscopes use refractive or GRIN lens with cross-section of several milimeters in diameter. Therefore, it is necessary to find less invasive probes to perform imaging in living organisms. Favourable alternative is to use multimode optical fiber probe with 100 micrometer diameter. This approach, however, requires advanced shaping of the wavefront in order to achieve diffraction limited imaging.
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3D printed microscopes
Žáková, Veronika ; Jákl, Petr (referee) ; Dostál, Zbyněk (advisor)
Modular microscope building kits allow those interested in microscopy to get a hands-on introduction to the construction of a light microscope. For this reason, it would be appropriate to equip the laboratories of the Institute of Physical Engineering at Brno University of Technology with such a kit. The inspiration can be found in the open source project openUC2, which offers optical kits based on a combination of 3D printed and commercially available optical components. The aim of my work is to use the open source parts database and supplement it with my own designs that would enable the construction of specialized polarization and fluorescence microscopes. The first part of the thesis introduces light microscopy methods and the equipment that is essential for their proper operation. Next, the 3D printing methods that are applicable to the fabrication of the designed parts are summarized. Beyond the openUC2, the design of a Köhler illuminator, a motorized stage and a motorized objective slider based on commonly available parts are introduced. A transmission microscope, a reflective polarizing microscope and a light sheet microscope were designed based on the UC2 kit with my construction modules. The first two have been assembled and tested along with their control software.
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Microscope for alignment of the optical fibers
Hekrlová, Kateřina ; Jákl, Petr (referee) ; Dostál, Zbyněk (advisor)
The demountable splicing of optical fibres uses different types of connectors which ensures accurate position of connected fibres. If the optical fibres are aligned in free space, a view from two perpendicular viewing directions is necessary for a maximum aligning accuracy. The method of direct monitoring of optical fibres provides this possibility however, it is necessary to use two imaging systems. This problem can be solved by a special microscope, which is designed in this thesis. The microscope can visualize the alignment of optical fibres from two mutually perpendicular directions by moving the objective lens and inclined mirror. The diploma thesis also describes the procedure of designing an optical simulation of the connection of optical fibres. Based on it, the microscope is designed, adjusted and tested with various optical fibres.
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3D printed opto-mechanical devices
Šremrová, Vendula ; Dostál, Zbyněk (referee) ; Jákl, Petr (advisor)
Optomechanical components are widely used in many optical experiments. This diploma thesis deals with design and manufacturing optomechanical components using 3D print technology. These are cheaper alternatives of commercial devices. In addition to 3D printed parts, minimum number of other components are used to assemble functional devices. Using simple experimental setups, the manufactured components are evaluated and compared with commercially available ones. The results show that they can be used in applications where high accuracy is not required. The second part is devoted to the design and manufacturing of a polarimeter as a mechanism combining electrical and mechanical components with 3D printed parts. The polarimeter is used to measure some properties of polarized light.
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Micropositioning tracking system
Svoboda, Tomáš ; Šerý, Mojmír (referee) ; Jákl, Petr (advisor)
This bachelor's thesis deals with the design and construction of a micro positioning device that will monitor the movement of a robotic mouse in a defined space with the help of a camera. The main task will be to assemble a moving platform, the aim of which will be to be in constant contact with the robot below it and to copy its 2D trajectory. The device will then be applied to a live mouse for in vivo holographic endoscopy experiments.
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Wavefront sensor
Škaroupka, David ; Jákl, Petr (referee) ; Dostál, Zbyněk (advisor)
When the light passes through optical elements it can cause deformation of wavefront light due to the damage of materials and optical aberrations. The deformation leads to unprecise imaging and deformation of an image. The diploma work mentions wavefront sensing techniques which are important for determination inappropriate errors of optical elements and the specification of their properties. The purpose of the master's thesis is to suggest and investigate wavefront sensing methods after the light passes through the optical element while using the digital micromirror device. The work deals with the topic of wavefront aberrations and geometric optical defects of optical elements. Different kinds of commercial products determined for wavefront sensing are described too.
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SMV-2019-67: Vector mode
Jákl, Petr ; Zemánek, Pavel
Preparation of experimental apparatus and control software for photopolymerization of nanostructures in vector mode. The nanostructures are created by continuous movement of the sample along a given trajectory with the laser beam switched on.
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