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"Frontáž". The Phenomenon of "frontáž" in Czech Art
Horáková, Markéta ; Polcar, Miloslav (advisor) ; Kornatovský, Jiří (referee)
Můj dědeček se věnoval numizmatice. Jako malá jsem ho mnohokrát pozorovala, jak svou sbírku mincí doplňuje a třídí. Snad abych ho nerozptylovala, naučil mě objevovat tajemství rubů i líců kovových penízků, z nichž jsem pak sama za pomoci tužky a papíru odkrývala neznámé reliéfhí předlohy. Myslím, že mi tato zábava i napětí vydržely hodně dlouho. Později, a to především díky svému otci, jsem obdobným způsobem získávala tvary a strukturu listů, květů i kůry stromů a vytvářela si tak svůj vlastní pseudoherbář. Ani děda, ani otec, ani já jsme však nikdy nepojmenovali naše otisky, respektive způsob jejich získávání, frotáží. S tímto slovem jsem se poprvé setkala až při studiu výtvarné výchovy na střední pedagogické škole, kde jsem, krom teoretického poučení, realizovala i několik praktických úkolů. Později, když jsem své žáky výtvarné výchově sama vyučovala a s touto technikou jsme pracovali, pozorovala jsem, že i oni přijímají frotáž se stejným zájmem jako já kdysi. Někdy jsme si uměle vytvořili motivy z lepenky - těmi byly například fragmenty stavebních prvků jihočeského selského baroka nebo zvětšeniny cechovních pečetí, které pak nalepené tvořily reliéfhí podklad pro frotáž. Jindy nám posloužily předlohy z přírody - listy, dřevo, kůra apod. A nakonec nemohu nepřipomenout kamenné náhrobky na zdejším židovském...
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Human subject in arts
Horáková, Markéta ; Kornatovský, Jiří (advisor) ; Synecký, Jakub (referee)
Teoretická práce se snaží přiblížit dobu 50. a 60. let let ve 20.st. a umění pop artu. Na příkladu hrdiny ukázat jak důležitý má význam nejen ve výtvarném umění a proč je Superman tak oblíbený. Didaktická část má za úkol pomocí hrdiny a příběhů přivést žáky zpět tradicím.
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Optimisation of SIA decarbazine determination
Horáková, Markéta ; Sklenářová, Hana (advisor) ; Chocholouš, Petr (referee)
Dacarbazine was determinated by sequential injection analysis with chemiluminiscence detection during reaction of sample and KMnO4 in sulfuric acid. Reagent volumes, their concentrations, flow rate and structure of cycle were optimised. Also the influence of solvents (water, 60% ethanol, 60% methanol) and enhancers of CL reaction (sodium polyphosphate, formic acid, glutaraldehyde and formaldehyde) was tested. Final optimal conditions are: 40 μl 1 M H2SO4, 3 μl 10 mM KMnO4, 30 μl 10 mM solution of dacarbazine, 40 μl 1 M H2SO4, eventually aspiration of 50 μl enhancer. Aspiration of the solutions proceeded in flow rate 100 μl/s, mixing and flow rate to the detector was 40 μl/s. Washing with oxalic acid was used after each measurement cycle to prevent KMnO4 settling on tubes. Voltage at the photomultiplier of the detector was 420 V. The calibration relations of CL intensity to concentration of the samples were nonlinear with possibility of selection of linear areas. The linear area for the solution of dacarbazine without enhancers wasn't found with correlation coefficient high enough, for the solution of dacarbazine with 60% methanol was 0.5 - 10 mmol/l, for the solution of dacarbazine with 1.39 M formic acid 4 - 10 mmol/l. Limit of detection for the solution of dacarbazine without enhancers was 0.1474...
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Study of chemiluminescence properties of metformin using sequential injection analysis
Horáková, Markéta ; Sklenářová, Hana (advisor) ; Chocholouš, Petr (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of analytical chemistry Candidate: Mgr. Markéta Horáková Consultant: PharmDr. Hana Sklenářová Ph.D. Title of Thesis: Study of chemiluminescence properties of metformin using sequential injection analysis Chemiluminescent properties of metformin were studied using a sequential injection analysis in the three systems with different composition of the reagents. The first system used potassium permanganate and sulfuric acid, the second cerium sulfate tetrahydrate and (tris (2,2 '-bipyridyl) dichloro-ruthenium hexahydrate. The third system used the N- bromsukcinimid and fluorescein. The flow rate, concentration and volumes of reagents, scheme of the measuring cycle and used solvents have been optimized into the first two systems. The concentration of N-bromosuccinimide (NBS) and fluorescein in the third system were based on data found in literature [Zhouping Wang, Anal. Lett. 2003]. The volume and speed were detected automatically using the program FaFSIAOptim, created the Department of Analytical Chemistry. Scheme of the measuring cycle was optimized in the same way as in the previous two systems. During the optimization, and subsequent measurements revealed that the only suitable system is the third system with NBS and...
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Nutritional status of patients hospitalized for diabetic foot syndrome - retrospective analysis
Horáková, Markéta ; Gojda, Jan (advisor) ; Krauzová, Eva (referee)
Diabetic foot syndrome is a serious chronic complication of diabetes mellitus, caused by a number of factors such as neuropathy, angiopathy, infection, and above all, this syndrome is consequence of a poor diabetes compensation and cardiovascular risk factors. It is characterized by particularly impaired wound healing and a higher tendency to chronicity, which can often be a reason for disability of the patient. Nutrition plays a very important role in a patient hospitalized with diabetic foot syndrome, as adequate coverage of energy and nutritional needs is essential for successful treatment and healing of ulcers. The theoretical part of this diploma thesis first mentions diabetes mellitus, its basic characteristics and briefly describes the types of diabetes that are associated with diabetic foot syndrome. Acute and chronic complications of diabetes are also discussed, followed by a more extensive chapter on the diabetic foot syndrome itself. This is the very definition of the term, epidemiology, pathogenesis, classification, diagnosis, treatment and prevention of this syndrome. The last chapter deals briefly with general nutritional recommendations for diabetics and the nutritional status of patients in which they are most often found when they are admitted to hospital, which is malnutrition,...
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Dřevokazné houby a jejich význam z pohledu údržby okrasných dřevin
Horáková, Markéta
This bachelor work addresses the issue of the expansion of wood-destroying fungi. It describes the theoretical part of this issue, basic composition of wood, feeding methods, description reproduction, spread of infection and general protection. Wooddecaying fungi are officials, attacking live trees, injured and dead. They cause various changes in wood and then decomposition woods. Furthermore, location was chosen, took place mapping injured trees and found species of woos-destroying fungi were determined. Follow up marking to own maps. Also care of trees been described to every species and the possibility of preventing the emergence of the disease.
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Correlation of Immunohistochemical and Molecular Methods in Diagnostics of Salivary Gland Tumors
Horáková, Markéta ; Skálová, Alena (advisor) ; Laco, Jan (referee) ; Dušková, Jaroslava (referee)
This doctoral thesis is dealing with the correlation of morphological, immunohistochemical and genetical findings in malignant tumors of salivary glands. The first half of the thesis comprises the summary of current knowledge about salivary malignancies. The second half is presenting the research itself. The research results are divided into three parts. The first part is presenting the method of "2-step diagnostic test" of malignant tumors. This screening test aims to find new, so far not described gene aberrations with a focus on malignant tumors of salivary glands. This method takes place in two consecutive steps. In the first step the material is examined by an immunohistochemical mixture of antibodies, which non-specifically detects aberration in the genes NTRK1-3, ALK and ROS1. In the second step all positive cases are subjected to highly sensitive and specific molecular-genetic examination by the method of next generation sequencing (NGS) using the Archer kit. In the second part of the work there has been designed the approach to the cytological diagnosis of salivary secretory carcinoma by the fine-needle aspiration (FNA). This part is describing to the details the cytomorphology of secretory carcinoma in both, Pap smears and cell blocks, from which additional immunocytochemical and genetic...
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Lifestyle of adolescents
Horáková, Markéta ; Aldhoon Hainerová, Irena (advisor) ; Zamrazilová, Hana (referee)
A healthy lifestyle plays an important role in both keeping an optimal body weight as well as in the prevention of cardiovascular or metabolic diseases in adulthood. Therefore, it is highly important for children and adolescents to avoid negative aspects of their lifestyles and to be educated and supported in leading a healthy lifestyle in all it's forms. The theoretical part of this bachelor thesis defines the terms adolescent and obesity. It also lists all important aspects of a healthy lifestyle, including recommendations related to nutritions and physical activity. Risk factors that may be associated with increased body weight, such as genetic factors, inappropriate eating habits, a lack of exercise, an adverse family and social background, selected diseases and long-term medication, are further discussed. The most common obesity-related complications in children and adolescents are also presented. Additionally, percentile graphs of assessed anthropometric parameters for boys and girls are shown. Recommendations for a healthier lifestyle during adolescence are finally listed. The goal of the practical part of the bachelor thesis was to ascertain what kind of lifestyle adolescents lead. Anonymous questionnaire research was carried out on 198 students (93 girls and 105 boys) in a private high...
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