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Business Plan
Škarková, Zuzana ; Fendrich, Zdeněk (referee) ; Rompotl, Jaroslav (advisor)
The theme of this thesis is to create the concrete project of development in selected entrepreneurial subject´s, which is here infocenter Turista from Pec pod Sněžkou. The work also contains of appropriate methods and procedures used in valuation of the company´s efficiency. The achieved results from implemented analysis hand information about the company´s situation and help to determine its supposed development.
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Receptor for Nicotinic Acid
Suchánková, Marie ; Fendrich, Zdeněk (advisor) ; Hronek, Miloslav (referee)
Nicotinic acid belongs to the drugs with anti-hyperlipidemia effects. It lowers levels of both cholesterol and triglycerides and increases the level of HDL. The benefit features of this vitamin are accompanied with the side effects of flushing, mainly, that have very negative influence to patient compliance. Independently, T. Soga and A. Wise have discovered the G-protein-coupled receptor GPR109A (HM74A in humans, PUMA-G in mice) as the receptor for nicotinic acid. This elucidation opened the way to elucidate the molecular identification of the mechanism of nicotinic acid-induced flushing. We have done several experiments with PUMA-G double-knockout mice which lacking the PUMA- G receptor. First of all, we have established the method for monitoring the flushing response after the administration of nicotinic acid and acipimox, its derivative with similar effect on the lipid metabolism. From our results, it implied that nicotinic acid and acipimox causes flushing response via the receptor PUMA-G. Thus we could say that PUMA-G receptors are involved in both lipid- lowering mechanism and flushing-mechanism. It has been postulated that NSAIDs can diminish the occurring flush response in the patients. Our experiments with COX-1 -/- clearly showed that COX-1 is one of the crucial enzymes in the nicotinic...
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Observations of dispensing of several drugs with dependence potential in Pharmacies
Zábranská, Kristýna ; Fendrich, Zdeněk (advisor) ; Vopršalová, Marie (referee)
Monitoring of the dispension of drugs with abuse potential in pharmacy Kristýna Zábranská Prof. MUDr. Zdeněk Fendrych Medication over the frame of standard treatment is a serious as well as underestimating problem. In the conseqeuncies it leads to hard medical and economic damage. Analysis of the dispension of prescription drugs with abuse potential (analgesics, benzodiazepines, nonbenzodiazepine hypnotics) in the pharmacy in 2006 confirmed that the consumption of this drugs is high, especially in the elderly (51 - 60 years). Group of the patients in this age was dispensed 72% of all dispensed drugs, that were monitoring. Monitored drugs were more frequently prescribed to women, in particular benzodiazepines. The three most prescribed substances were diazepam, alprazolam and bromazepam. In this sequence they were dispensed to women too. To the group of men there was the most often prescribed substance diazepam, zolpidem (Hypnogen) and tramadol (Tramal).
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Molecular biological evidence of Pneumocystis jirovecii (carinii) with application of the real-time PCR
Roučková, Vítězslava ; Fendrich, Zdeněk (advisor) ; Vopršalová, Marie (referee)
Pneumocystis carinii is an opportunistic pathogen, which often causes fatal pneumonia in patients under immunosuppressed or immune deficient conditions due to AIDS, cancer chemotherapy, or immunosuppressive therapy for organ transplantation. Different techniqes of microscopy and a nested polymerase chain reaction (PCR) are widely used for detection of this opportunistic fungus. But these methods are less sensitive and time-consuming. We focused our attention on the level of specific DNA by a quantitative PCR technique. This procedure has the advantage of greater precision and more objectivity. In this report we describe a real-time PCR assay suitable for use with the LightCycler system. We were successful in implementation and optimalization of quantitative real-time PCR for Pneumocystis carinii. We have reached sensitivity 1000 copies of DNA Pneumocystis carinii /ml. We have set a limit of detection to be 50 copies of DNA Pneumocystis carinii /ml. Real-time PCR procedure was optimized and validated in laboratory of molecular biology of Department of clinicall biochemistry and diagnostics of Fakultni Nemocnice in Hradec Kralové. A standard routine was established to be utilized in general practice
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Účinky rostlinných extraktů z bazalky, jalovce a vavřínu na životaschopnost SH-SY5Y buněk neuroblastomu
Závadová, Vlasta ; Tilšer, Ivan (advisor) ; Fendrich, Zdeněk (referee)
Cancer is one of the major causes of death in the world, especially in developed countries. There are already many antiproliferative agents used to cure patients with cancer. However, many of the currently approachable medicines are synthetic chemicals, which are invasive and very often cause alterations in healthy cells. Because of these reasons, there is a tendency to turn back to natural agents used mainly traditionally in folk medicine, because some of them might be potentially effective against tumor development. In this study, the cytotoxic effects of basil, laurel and juniper extracts on neuroblastoma cancer cells were tested by using MTT and LDH assays. The extracts at concentrations 2; 0,5; 0,1; 0,05 and 0,01 mg/ml were effecting the cells for 12 hours. Another method used for studying of extract's effects was western blotting analysis showing the level of tumor supressor p53 protein in the neuroblastoma cells after extract treatment. The results with treated cells were compared to the untreated cells serving as a control. The results of MTT assay show decrease in cell viability of cells treated with all three plant extracts at concentration 2 mg/ml. Also four other concentrations of laurel and juniper extracts 0,5; 0,1; 0,05 and 0,05 mg/ml highly decreased the cell viability in comparison...
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