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Methodology for analysis of dairy products and their soy analogues for the presence of RoundUp Ready soybean
Tesařová, Zuzana ; Šídová, Dana ; Vráblík, Aleš ; Hodek, Jan ; Ovesná, Jaroslava
Dairy products may be subject to adulteration, where there is a relatively expensive raw material (e.g. cream) replaced by the addition of vegetable fats. Milk can be replaced in dairy products for example by soybean extract. Because soy belongs among strong allergens and the incidence of allergy is rather high. Therefore it is necessary to have a method for its detection. This work introduces the method for detection of soy, including analysis of genetic modified RoundUp Ready soybean. Detection of soybean in dairy products is based on analysis of amplification products melting profiles by High Resolution Melting (HRM) method. Analysis of geneticaly modified RoundUp Ready soybean is based on DNA detection via Polymerase Chain Reaction(PCR).
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Alternative nitrogen nutrition of plants
Mikanová, Olga ; Šimon, Tomáš
The methodology provides the basic information about the usability of nitrogenfixating bacteria. The methodology describes procedures of the isolation and screening of beneficial soil bacteria Azotobacter spp. and Rhizobium spp. It also provides recommendations to promote biological nitrogen fixation. The methodology also represents the use of inoculants as a necessary part of modern farming practices.
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Methodology for the phenotyping resistance to Plum pox virus in peach
Salava, Jaroslav ; Polák, Jaroslav
Sharka disease, which is caused by the Plum pox virus (PPV), is the most economically important virus disease in fruit trees worldwide. Since it is not possible to completely eradicate PPV in the Czech Republic, it is necessary to find another way to control this pathogen. The most promising seems to be the breeding of new cultivars for resistance. This methodology provides a comprehensive protocol for testing PPV resistance in peach. It is based on visual assessment of disease symptoms on leaves and fruits, and determining the presence of the virus in plants using ELISA and RT-PCR techniques.
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The Efficient Application of Near Infrared Spectroscopy with Fourier Transformation for Prediction of Wheat Technological Parameters
Dvořáček, Václav ; Prohasková, Anna ; Štočková, Lenka
This method brings to the users basic information about principles and features of NIR spectroscopy as well as more detailed view of the state-of-the-art capabilities of NIR spectroscopy in wheat quality prediction. The agriculture professionals will use supportive information about possible utilization of the method including practical tips for proper NIR spectrometer selection. For both, the current and prospective users, the method provides practical guidance to device handling, management of calibration sets and calibration models development. The objective evaluation of the 21 predictive models based on FT-NIR measurement, covering the results of 14 reference analytical methods aimed at wheat quality. Furthermore, it offers information useful directly in breeding programs, mill and bakery production and also in agriculture sector, government control and at universities.
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Cultivation of fodder sorrel for biogas production
Usťak, Sergej
The aim of the methodology is to provide farmers, agricultural consultants, operators of biogas plants and all other interested persons basic information about growing and processing for biogas of a promising non-traditional energy crop - fodder sorrel (Rumex patientia L. x Rumex tianschanicus A.Los.). Until now a comprehensive methodology for cultivation of this crop for biogas production hasn’t been processed. The methodology provides basic botanical characteristics of the crop, specifies soil and ecological requirements in habitat, describes the agricultural technologies of seeding, cultivation, fertilization, plant protection, harvesting and ensiling this crop for biogas production and provides their economic evaluation.
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Possibilities of cultivation of cup-plant Silphium perfoliatum L. for biogas production
Usťak, Sergej
The aim of the methodology is to provide farmers, agricultural advisors, providers of biogas stations and anyone interested in growing and processing of agricultural biomass as a renewable source of raw materials and energy the basic information about a prospective non-traditional crop – cup-plant Silphium perfoliatum L. Up to now, a comprehensive methodology for cultivation this crop in conditions of the Czech Republic is not processed. The publication provides basic botanical characteristics of the crop, specifies its soil and ecological habitat requirements, describes the agricultural technologies for seeding, cultivation, fertilization, plant protection, harvesting and post-harvest treatment and gives an overview of potential productivity and main utilization possibilities, especially for biogas.
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Cultivation and use of eastern goat's rue Galega orientalis Lamb. in the Czech Republic
Usťak, Sergej ; Váňa, Vojtěch
The aim of the methodology is to provide farmers, agricultural advisors and anyone interested in growing and processing of agricultural biomass as a renewable source of raw materials and energy the basic information about a prospective non-traditional crop - eastern goat's rue Galega orientalis Lamb. Up to now, a comprehensive methodology for cultivation this crop in conditions of the Czech Republic is not processed. The publication provides basic botanical characteristics of the crop, specifies its soil and ecological habitat requirements, describes the agricultural technologies for seeding, cultivation, fertilization, plant protection, harvesting and post-harvest treatment and gives an overview of potential productivity and main utilization possibilities, especially for biogas. The importance of this legume for soil fertility is also emphasized.
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Sorghum (Sorghum bicolor (L.) MOENCH) and its utilization possibilities in the condition of the Czech Republic
Hermuth, Jiří ; Janovská, Dagmar ; Strašil, Zdeněk ; Usťak, Sergej ; Hýsek, Josef
Sorghum is one of the oldest used crops. Presently, it is one of the five most cultivated cereals in the world for human nutrition. This methodology includes a comprehensive view of sorghum cultivation and presents the newest knowledge in range from botanical taxonomy to the possibilities of utilization. In this publication, there are included not only our own results but there are also results from the world scientific publications. There are characterized selected species of sorghum suitable for different climatic conditions of the Czech Republic. There are described all aspects and procedures, which can influence the final production of selected sorghum varieties including their quality from the point of view of biogas production.
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