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Diagnostic protocol for Erwinia amylovora, the causal agent of fire blight
Kokošková, B. ; Mráz, Ivan
The aim of this methodology is optimized diagnostic protocol for detection and determination of the causal agent of fire blight, quarantine bacterium E. amylovora. The disease causes high economic losses in plantations of pome-fruit trees and susceptible ornamental plants, in particular in Crataegus sp. and Cotoneaster sp. Diagnostic protocol is based on a combination of both optimized techniques utilizing different principles of action: immunochemical (immunofluorescent test) and molecular (polymerase chain reaction). The submitted diagnostic protocol alows to eliminate potential false negatives and positives and ensure the highest reliability of results. Specific and timely detection of E. amylovora alows effective application of plant protection measures.
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