National Repository of Grey Literature 20 records found  1 - 10next  jump to record: Search took 0.00 seconds. 
Methodology of Diagnostics of Pectobacterium atrosepticum and Dickeya solani using Real-Time PCR.
Kmoch, M. ; Binderová, D. ; Kopačka, V. ; Vacek, J. ; Brázdová, S. ; Petrzik, Karel ; Ševčík, R.
The methodology describes the optimization of the molecular diagnostic method of Pectobacterium atrosepticum and Dickeya solani based on real-time PCR and SybrGreen real-time PCR, including sample preparation, DNA isolation, specificity control and evaluation.
Plant virus-based biotechnology
Vaculík, Petr ; Čeřovská, Noemi (advisor) ; Ryšánek, Pavel (referee) ; Petrzik, Karel (referee)
The latest model of tertiary structure of capsid protein of potato virus X (PVX CP) was used as a template to design new insertion sites suitable for the preparation of PVX-based antigen presentation system. Based on this model, seven insertion sites (A-G) located in putative surface loops were tested. As an antigen inserted into these sites was used 17 amino acids long epitope derived from human papillomavirus type 16 E7 oncoprotein (E7 epitope) fused with either 6xHis tag or StrepII tag in both possible orientations (6xHis-E7 and E7-6xHis, StrepII-E7 and E7-StrepII). Prior to plant expression, modified PVX CPs were expressed in Escherichia coli MC1061. The results showed that only PVX CP carrying StrepII-E7 or E7-StrepII in the insertion site A formed virus particles. The results from transient expression experiments with modified PVX CPs in Nicotiana benthamiana showed that only the insertion site A (located between 24th and 25th amino acid in the PVX CP) could tolerate all tested inserts. Importantly, viral particles were detected only in the presence of StrepII tag and their stability was affected by the insert orientation (StrepII-E7 vs. E7-StrepII) as only the viral particles presenting E7-StrepII could be purified. Besides the preparation of PVX-based antigen presentation system, an...
Cultivated and wild growing forage crops – reservoirs of viruses and phytoplasmas
Fránová, Jana ; Petrzik, Karel ; Jakešová, H. ; Bečková, M. ; Sarkisova, Tatiana
Symptoms of mosaic, yellowing/reddening, phyllody, necrosis and dwarf have been observed in cultivated as well as wild growing forage crops. Red clover mottle virus (RCMV, Comovirus), clover yellow vein virus (Potyvirus), potato virus X, white clover mosaic virus (WClMV, Potexvirus), alfalfa latent and pea streak viruses (Carlavirus), new members of genus Cytorhabdovirus and Carlavirus were identified by sequencing. Bacilliform particles (ca 213 – 533 nm by 44 – 58 nm) of the genus Cytorhabdovirus, WClMV, alfalfa mosaic virus (Alfamovirus) and particles resembling members of the genus Badnavirus were observed on negatively stained preparations and ultrathin sections by electron microscopy and mechanically transmitted to host plants. WClMV, RCMV, bean yellow mosaic virus and new member of Potyviridae family were determined in wild growing plants. The occurrence of phytoplasmas belonging to different 16S ribosomal subgroups was confirmed (16SrI-B, 16SrI-C, 16SrX-A, 16SrXII-A).

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