National Repository of Grey Literature 14 records found  1 - 10next  jump to record: Search took 0.01 seconds. 
Using of divergent flow isoelectric focusing and capillary liquid
Duša, Filip ; Moravcová, Dana ; Kahle, Vladislav ; Šlais, Karel
Separation of analytes from complex matrices play important role in their further identification. In this article we propose two-dimensional separation method of bovine serum albumin tryptic peptides. At the first dimension peptides are separated by divergent flow isoelectric focusing followed by reverse-phase liquid chromatography as the second dimension. Due to composition of input mixture it is possible to use autofocusing mode in first dimension. In this mode peptides are focused without addition of carrier ampholytes. Chromatograms from the second dimension were processed into contour map. Acquired data showed good separation efficiency of isoelectric focusing. Autofocusing mode enabled better resolution of peptides with close isoelectric points.
Electrophoretic separation of oligosaccharides with conductivity detection
Partyka, Jan ; Foret, František
Improvement of separation properties of oligosaccharides by quaternary ammonium labeling.
Differentiation of Candida strains by capillary HPLC
Vykydalová, Marie ; Moravcová, Dana ; Horká, Marie ; Růžička, F. ; Kahle, Vladislav
The aim of this study was to compare chromatographic profiles of lysate of Candida parapsilosis positive and Candida parapsilosis negative using capilary high performance liquid chromatography.
CECE 2011. 8th International Interdisciplinary Meeting on Bioanalysis
Foret, František
Sborník obsahuje program, abstrakta 17 zvaných přednášek s krátkou informací o profesionální dráze řečníků a abstrakta posterů.
Preparation of highly luminescent CdTe quantum dots and its probes
Voráčová, Ivona ; Lišková, Marcela ; Patrmanová, M. ; Klepárník, Karel ; Foret, František
Luminescence probes consisting of quantum dots conjugated with macrocyclic ligand complex were characterized by CE-LIF.
Immunofluorescent labeling of tissues and free cells via QD-protein conjugates
Lišková, Marcela ; Voráčová, Ivona ; Hezinová, Věra ; Klepárník, Karel ; Foret, František
Preparation of quantum dots and using their QD-protein conjugates in immunofluorescent labeling of cells and tissues. For conjugation was used method viaEDC/sulfo-NHS zero-length cross-linkers. We linked QD with annexin V, anti-CD3 and anti-PCNA. We labeled T-lymphocytes, apoptotic lymphocyte, mouse duodenum tissue and tumor tissue.
Preparation, physical-chemical properties and utilization of nanoparticles in bioanalysis
Svobodová, Ivona ; Hezinová, Věra ; Lišková, Marcela ; Přikryl, Jan ; Maděránková, D. ; Klepárník, Karel ; Foret, František
The possibilities of application of nanoparticles in bioanalysis were studied. Applications of quantum dots as a fluorescent markers, silver nanoparticles in SERS and conjucation of gold nanoparticles with antibodies were shown.
Application of nanoparticles in fluorescence microscopy and raman spectrometry
Přikryl, Jan ; Svobodová, Ivona ; Hezinová, Věra ; Lišková, Marcela ; Maděránková, D. ; Klepárník, Karel ; Foret, František
This contribution is devoted to application of quantum dots as perspective luminescence labels in fluorescence microscopy (epifocal and TIR fluorescence microscopy) and application of silver nanoparticles in surface-enhanced Raman spectroscopy.
CdTe kvantové tečky - příprava, fyzikálně-chemické vlastnosti a aplikace jako fluorescenční značky
Hezinová, Věra ; Svobodová, Ivona ; Lišková, Marcela ; Přikryl, Jan ; Klepárník, Karel ; Foret, František
The set of different sizes of CdTe nanoparticles coated with MPA was synthetized and characterized by fluorescence spectra and lifetimes. Their application as a cell fluorescent markers was tested.
Potenciál kvantových teček při detekci jediné molekuly v buňkách a organelách
Přikryl, Jan ; Klepárník, Karel ; Svobodová, Ivona ; Hezinová, Věra ; Lišková, Marcela ; Preisler, J. ; Foret, František
This contribution is devoted to quantum dots usage for cell imaging in fluorescence microscopy. Fluorescence excitation by evanescence wave (caused by total internal reflection) is compared with conventional epifluorescence microscopy.

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